Surface-enhanced Raman scattering (SERS) revealing chemical variation during biofilm formation: from initial attachment to mature biofilm

Chao, Yuanqing; Zhang, Tong

doi:10.1007/s00216-012-6225-y

Cited by 126 publications

(117 citation statements)

References 53 publications

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“…Monitoring the structural changes in biofilms at different time points of their growth with SERS can provide in-depth understanding of biofilm formation processes. In this context, Chao et al [54] studied the formation Escherichia coli, Pseudomonas putida, and Bacillus subtilis biofilms between 0 and 72 h of growth with SERS using hydroxylamine hydrochloride-reduced AgNPs. Biofilms were grown on quartz windows and then 200 µL AgNPs was added on the substrates, which were incubated until dry before SERS measurements.…”

Section: Identification Of Spectral Signatures In a Biofilmmentioning

confidence: 99%

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Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

2018

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Biofilms are a communal way of living for microorganisms in which microorganism cells are surrounded by extracellular polymeric substances (EPS). Most microorganisms can live in biofilm form. Since microorganisms are everywhere, understanding biofilm structure and composition is crucial for making the world a better place to live, not only for humans but also for other living creatures. Raman spectroscopy is a nondestructive technique and provides fingerprint information about an analyte of interest. Surface-enhanced Raman spectroscopy is a form of this technique and provides enhanced scattering of the analyte that is in close vicinity of a nanostructured noble metal surface such as silver or gold. In this review, the applications of both techniques and their combination with other biofilm analysis techniques for characterization of composition and structure of biofilms are discussed.

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Section: Identification Of Spectral Signatures In a Biofilmmentioning

confidence: 99%

“…Identification of the changes in the spectral pattern during biofilm formation [54] Algae and Pseudomonas aeruginosa…”

Section: Type Of Microorganism Outputmentioning

confidence: 99%

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

2018

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“…The investigation of microorganisms [13] as well as the examination of biofilm formations [1,[14][15][16] by using Raman microspectroscopy has currently become quite popular. Components such as DNA, RNA, proteins, lipids, and carbohydrates of the bacterial cells may vary and, therefore, cause the Raman spectra of two different species to be slightly different which can be successfully used for species discrimination [17,18].…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopic differentiation of planktonic bacteria and biofilms

et al. 2015

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Both biofilm formations as well as planktonic cells of water bacteria such as diverse species of the Legionella genus as well as Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were examined in detail by Raman microspectroscopy. Production of various molecules involved in biofilm formation of tested species in nutrient-deficient media such as tap water was observed and was particularly evident in the biofilms formed by six Legionella species. Biofilms of selected species of the Legionella genus differ significantly from the planktonic cells of the same organisms in their lipid amount. Also, all Legionella species have formed biofilms that differ significantly from the biofilms of the other tested genera in the amount of lipids they produced. We believe that the significant increase in the synthesis of this molecular species may be associated with the ability of Legionella species to form biofilms. In addition, a combination of Raman microspectroscopy with chemometric approaches can distinguish between both planktonic form and biofilms of diverse bacteria and could be used to identify samples which were unknown to the identification model. Our results provide valuable data for the development of fast and reliable analytic methods based on Raman microspectroscopy, which can be applied to the analysis of tap water-adapted microorganisms without any cultivation step.

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“…At biointerfaces, for instance, the distribution of small functional ions such as , or small organic molecules such as glucose and phospholipid molecules remains unclear. This is despite the use of many in situ techniques such as atomic force microscopy [1,2], fluorescence [3,4], Raman [5], infrared [6,7], and surface plasmon resonance [8].…”

mentioning

confidence: 99%

Improving the Molecular Ion Signal Intensity for In Situ Liquid SIMS Analysis

Zhou

Yao

Ding

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. In situ liquid secondary ion mass spectrometry (SIMS) enabled by system for analysis at the liquid vacuum interface (SALVI) has proven to be a promising new tool to provide molecular information at solid-liquid and liquid-vacuum interfaces. However, the initial data showed that useful signals in positive ion spectra are too weak to be meaningful in most cases. In addition, it is difficult to obtain strong negative molecular ion signals when m/z>200. These two drawbacks have been the biggest obstacle towards practical use of this new analytical approach. In this study, we report that strong and reliable positive and negative molecular signals are achievable after optimizing the SIMS experimental conditions. Four model systems, including a 1,8-diazabicycloundec-7-ene (DBU)-base switchable ionic liquid, a live Shewanella oneidensis biofilm, a hydrated mammalian epithelia cell, and an electrolyte popularly used in Li ion batteries were studied. A signal enhancement of about two orders of magnitude was obtained in comparison with non-optimized conditions. Therefore, molecular ion signal intensity has become very acceptable for use of in situ liquid SIMS to study solid-liquid and liquid-vacuum interfaces.

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Surface-enhanced Raman scattering (SERS) revealing chemical variation during biofilm formation: from initial attachment to mature biofilm

Cited by 126 publications

References 53 publications

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

Raman spectroscopic differentiation of planktonic bacteria and biofilms

Improving the Molecular Ion Signal Intensity for In Situ Liquid SIMS Analysis

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