Surface-enhanced Raman Scattering (SERS) microbial sensor for fresh water acute toxicity monitoring

Gao, Weixing; Duan, Weiman; Peng, Dandan; Li, Juan; Hu, Zhangmei; Wang, Dongmei; Gong, Zhengjun; Fan, Meikun

doi:10.1016/j.microc.2023.108822

Cited by 4 publications

(6 citation statements)

References 49 publications

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“…The PC1 score increased progressively with an increasing concentration of toxicants. The inhibition rate is used to assess toxicity and calculated according to eq : Inhibition false( % false) = false( PC λ − PC 0 false) / false( PC ∞ + false| PC 0 false| false) PC λ and PC 0 represent the PC1 scores of bacteria under Cu 2+ exposure and without Cu 2+ exposure, respectively. PC ∞ represents the PC1 score of bacteria under the maximum concentration of Cu 2+ exposure set in the experiment (bacterial inactivation at this concentration).…”

Section: Methodsmentioning

confidence: 69%

“…A comparison of the SERS microbial biosensor for Cu 2+ toxicity detection with other reported methods is shown in Table S1. It is important to remember that the sensitivity of biotoxicity assays depends on the chosen detection procedure, the specific microorganisms used, and the toxicity end points being measured. ,,,− Generally, lower IC 50 values are corresponded to higher sensitivity of toxicity assays . However, it is worth noting that the SERS-based method offers significant advantages in terms of operational simplicity and speed (analysis time reduced from 24 h to under 1 h).…”

Section: Resultsmentioning

confidence: 99%

“…The PC1 score increased progressively with an increasing concentration of toxicants. The inhibition rate is used to assess toxicity and calculated according to eq 1 19 :…”

Section: Instrumentsmentioning

confidence: 99%

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Label-Free Surface-Enhanced Raman Scattering-Based Microbial Whole-Cell Biosensor for Acute Toxicity Assessment in Water

Ma,

Liu,

Zhou

et al. 2024

ACS EST Water

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Aquatic toxicity assessment is extremely important to ensuring water safety. Typically, the evaluation was accomplished by introducing bioluminescent bacteria, where the weakened luminescence was used to calculate the acute toxicity. Herein, a new microbial wholecell biosensor was proposed for aquatic toxicity evaluation using a labelfree surface-enhanced Raman spectroscopy (SERS) technique with Escherichia coli (E. coli) as a model organism. The proposed strategy required neither special bacteria strains nor mediators. The dosedependent SERS signal of the exposed model bacteria can be directly acquired. For the model toxic chemical Cu 2+ , the half-inhibitory concentration (IC 50 ) was found to be 3.87 mg/L. The method also helps understand the mechanism of toxicity of bacterial cells after exposure to a toxic environment, providing a multiparameter view of the changes in bacterial physiological metabolism induced by toxicants.

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Section: Methodsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Label-Free Surface-Enhanced Raman Scattering-Based Microbial Whole-Cell Biosensor for Acute Toxicity Assessment in Water

Ma,

Liu,

Zhou

et al. 2024

ACS EST Water

Self Cite

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“…Surface-enhanced Raman scattering (SERS), a powerful surface analytical technique, has wide applications in food safety control, 1,2 environmental monitoring, 3,4 and biotechnology, 5,6 among many others. SERS amplifies Raman scattering signals of molecules adsorbed onto metal nanostructures to achieve high sensitivity with characteristic molecular fingerprints.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Surface-enhanced Raman scattering (SERS), a powerful surface analytical technique, has wide applications in food safety control, , environmental monitoring, , and biotechnology, , among many others. SERS amplifies Raman scattering signals of molecules adsorbed onto metal nanostructures to achieve high sensitivity with characteristic molecular fingerprints. , Metal nanostructures are typically built on rigid substrates, such as silicon (Si) wafer, , glass, , and metal sheet. , The advantage of solid rigid substrates lies in the high precision and ease of control over metal architectural nanostructures.…”

Section: Introductionmentioning

confidence: 99%

Flexible Surface-Enhanced Raman Scattering Tape Based on Ag Nanostructured Substrate for On-Site Analyte Detection

Wu,

Kanike,

Marcati

et al. 2024

Langmuir

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Surface-enhanced Raman scattering (SERS) has emerged as a powerful surface analytical technique that amplifies Raman scattering signals of molecules adsorbed onto metal nanostructured surfaces. The droplet reaction method has recently been employed to fabricate large-scale microring patterns of silver (Ag) nanostructures on rigid substrates, which enables sensitive detection within the ring area. However, these rigid substrates present limitations for direct on-site detection of analyte residues on irregular sample surfaces. There is a need to develop soft and flexible SERS substrates that can intimately conform to arbitrary surfaces. In this study, we presented a SERS substrate using flexible and adhesive tape as the supporting material. This SERS tape was fabricated by repeatedly transferring presynthesized Ag nanostructures from a rigid substrate to the tape. For a model compound adenine, our SERS tape exhibited a good linear response from 5 × 10 −4 M to 5 × 10 −5 M with a low limit of detection (LOD) of 5 × 10 −7 M and displayed a SERS enhancement factor (EF) of 3.2 × 10 5 . The relative standard deviation (RSD) of SERS intensity achieved was as low as 1.93%, indicating its outstanding uniformity. The as-prepared SERS tape was used for in situ detection of pesticide residue on an apple surface and dye residue on human hair. Leveraging the large surface area of Ag nanostructure patterns from the droplet reaction, the developed SERS tape demonstrates excellent performance in terms of sensitivity and uniformity. The successful detection of analyte residues on arbitrary surfaces of apple and human hair highlights the potential of this flexible SERS tape for real-world applications across various industries for enhanced diagnostic accuracy.

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Prepared Plasmonic Glass Substrates via Electrodeposition for Detecting Trace Glucose: SERS, DFT, and FDTD Investigations

Sahbafar,

Mehmandoust,

Zeinalizad

et al. 2023

Plasmonics

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Surface-enhanced Raman Scattering (SERS) microbial sensor for fresh water acute toxicity monitoring

Cited by 4 publications

References 49 publications

Label-Free Surface-Enhanced Raman Scattering-Based Microbial Whole-Cell Biosensor for Acute Toxicity Assessment in Water

Label-Free Surface-Enhanced Raman Scattering-Based Microbial Whole-Cell Biosensor for Acute Toxicity Assessment in Water

Flexible Surface-Enhanced Raman Scattering Tape Based on Ag Nanostructured Substrate for On-Site Analyte Detection

Prepared Plasmonic Glass Substrates via Electrodeposition for Detecting Trace Glucose: SERS, DFT, and FDTD Investigations

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