Surface enhanced raman scattering of biomolecules

Koglin, E.; Séquaris, J.‐M.

doi:10.1007/3-540-16403-0_1

Cited by 116 publications

(85 citation statements)

References 159 publications

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“…This is explained in Ref. 23 by the high sensitivity of the SERS method only for the macromolecular components coming into direct contact with the surface (in this case adenine).…”

Section: Comments On Proposed Sm-sers Mechanismsmentioning

confidence: 99%

What is observed in single molecule SERS, and why?

Otto

2002

J Raman Spectroscopy

230

177

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Proposed single molecule surface-enhanced Raman scattering (SM-SERS) mechanisms are discussed and problems in SM-SERS of biological molecules are pointed out. It is unlikely that the single molecule signals can be explained exclusively by electromagnetic (EM) 'hot spots' in fractal structures or in narrow gaps. Care must be taken to recognize and avoid the signal of sp 2 -carbon contamination, the origins of which are chemical impurities and/or photophysical reactions of the chosen adsorbate. As already pointed out by Käll and co-workers, relatively large globular proteins fit only in wider gaps, and therefore the EM enhancement is several orders smaller than often reported enhancements in SM-SERS. SM-SERS of haemoglobin probably shows signals from the Fe-porphyrin constituent among other unknown signals. It cannot be excluded that the haemoglobin is denatured and the haem group is situated in a smaller gap.

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“…This is explained in Ref. 23 by the high sensitivity of the SERS method only for the macromolecular components coming into direct contact with the surface (in this case adenine).…”

Section: Comments On Proposed Sm-sers Mechanismsmentioning

confidence: 99%

What is observed in single molecule SERS, and why?

Otto

2002

J Raman Spectroscopy

230

177

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“…The reason lies probably in the comparatively low scattering cross sections of the sugar and phosphate groups attached to the bases. Especially for SERS measurements of double-stranded DNA, this is a major challenge, 14 as the sugar and phosphate groups shield the bases from direct contact with the SERS-active metal particles or probes. To overcome this problem, especially important for the identification of particular sequences, different approaches have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Surface‐ and tip‐enhanced Raman scattering of DNA components

Rasmussen

Deckert

2006

J Raman Spectroscopy

185

161

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While surface-enhanced Raman scattering (SERS) is an established technique to significantly enhance the Raman signal from samples bound to metal surfaces, a quantitative interpretation is very difficult because of the rough and arbitrary nature of the substrate. A distinction between concentration and enhancement effects is virtually impossible. Tip-enhanced Raman scattering (TERS), a scattering type of near-field optical microscopy technique, circumvents the problem of random enhancement by concentrating the roughness to only a small region directly at the apex of a scanning probe tip. In this paper, TERS spectra of DNA pyrimidine bases have been collected and compared with standard SERS and Raman measurements of the nucleotides and the pure bases. The results are discussed in terms of electromagnetic and chemical effects of the SERS enhancement of the TERS probes.

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“…Numerous studies suggest that the SERS active sites are likely adatoms, atomic clusters, sharp steps, or edges [31,32]. They are responsible for chemical enhancement via resonant charge-transfer and resonance-Raman-like enhancement [33].…”

Section: Sers Active Sitesmentioning

confidence: 99%

Surface Enhanced Raman Scattering (SERS): From Fundamental Mechanism to Bio-Analytics Tools

Zhi¹,

Liu²,

Chen³

2016

Nano BioMed ENG

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This review discusses the surface-enhanced Raman scattering (SERS) with efficiencies enhanced by as much as 10 14 -10 15 fold as well as a SERS cross section on the order of 10 -17 -10 -16 cm 2 / molecule. Despite the existing controversy on the origin of the enhancement, SERS effect offers new opportunities for spectroscopic detection of single molecules near or on the surface of metallic nanoparticles. There has been a considerable amount of research on explanation of the enhancement mechanism and on developing SERS labels as bio-analytics tools for either molecular multiplexed detection or bio-imaging at different levels.

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Surface enhanced raman scattering of biomolecules

Cited by 116 publications

References 159 publications

What is observed in single molecule SERS, and why?

What is observed in single molecule SERS, and why?

Surface‐ and tip‐enhanced Raman scattering of DNA components

Surface Enhanced Raman Scattering (SERS): From Fundamental Mechanism to Bio-Analytics Tools

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