Surface enhanced Raman scattering of extracellular vesicles for cancer diagnostics despite isolation dependent lipoprotein contamination

Koster, Hanna J.; Rojalin, Tatu; Powell, Alyssa; Pham, Dina; Mizenko, Rachel R.; Birkeland, Andrew C.; Carney, Randy P.

doi:10.1039/d1nr03334d

Cited by 34 publications

(35 citation statements)

References 79 publications

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“…One study reports on the adsorption of extracellular vesicles (EVs) and lipoproteins on a quartz microfiber matrix embedded with AuNPs on borosilicate glass after exposure to cysteamine solution. The resulting EVs substrate was used for SERS and SEM investigations using treatment with glutaraldehyde (15 min), osmium tetroxide (15 min), and a series of water/ethanol solutions [ 61 ]. Moreover, glass substrates coated with 30 nm AuNPs using the convective self-assembly (CSA) method were used for SERS detection of 4-ABT (LOD 4.7 nM, EF 1.34 × 10 5 ) and LSPR detection of IgG (500 nM) with 211% sensitivity improvement vs. continuous gold-coated glass.…”

Section: Optical Immunosensorsmentioning

confidence: 99%

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

Ionescu

2022

Biosensors

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Currently, several biosensors are reported to confirm the absence/presence of an abnormal level of specific human biomarkers in research laboratories. Unfortunately, public marketing and/or pharmacy accessibility are not yet possible for many bodily fluid biomarkers. The questions are numerous, starting from the preparation of the substrates, the wet/dry form of recognizing the (bio)ligands, the exposure time, and the choice of the running buffers. In this context, for the first time, the present overview summarizes the pre-functionalization of standard and nanostructured solid/flexible supports with cysteamine (Cys) and glutaraldehyde (GA) chemicals for robust protein immobilization and detection of biomarkers in body fluids (serum, saliva, and urine) using three transductions: piezoelectrical, electrochemical, and optical, respectively. Thus, the reader can easily access and compare step-by-step conjugate protocols published over the past 10 years. In conclusion, Cys/GA chemistry seems widely used for electrochemical sensing applications with different types of recorded signals, either current, potential, or impedance. On the other hand, piezoelectric detection via quartz crystal microbalance (QCM) and optical detection by surface plasmon resonance (LSPR)/surface-enhanced Raman spectroscopy (SERS) are ultrasensitive platforms and very good candidates for the miniaturization of medical devices in the near future.

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Section: Optical Immunosensorsmentioning

confidence: 99%

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

Ionescu

2022

Biosensors

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“…Notably, blocking of PD-L1+ exosome signaling to PD-1+ T-cells attenuated immune suppression. This research provides further evidence that circulating PD-L1 expression on EXOs may be harnessed as a future non-invasive biomarker and used to monitor for the presence of disease, tumor stage, and progression [ 120 ].…”

Section: Future Directions and Challengesmentioning

confidence: 99%

The Promise of Circulating Tumor DNA in Head and Neck Cancer

Aulakh

Silverman

Young

et al. 2022

Cancers

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As the seventh most common cancer globally, head and neck cancers (HNC) exert considerable disease burden, with an estimated 277,597 deaths worldwide in 2020 alone. Traditional risk factors for HNC include tobacco, alcohol, and betel nut; more recently, human papillomavirus has emerged as a distinct driver of disease. Currently, limitations of cancer screening and surveillance methods often lead to identifying HNC in more advanced stages, with associated poor outcomes. Liquid biopsies, in particular circulating tumor DNA (ctDNA), offer the potential for enhancing screening, early diagnosis, and surveillance in HNC patients, with potential improvements in HNC patient outcomes. In this review, we examine current methodologies for detecting ctDNA and highlight current research illustrating viral and non-viral ctDNA biomarker utilities in HNC screening, diagnosis, treatment response, and prognosis. We also summarize current challenges and future directions for ctDNA testing in HNC patients.

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“…Recently, Koster et al. demonstrated the ability of SERS-based analysis combined with machine learning algorithms to detect cancer-derived sEVs despite contamination with lipoproteins [ 181 ]. These authors isolated sEVs by known isolation techniques including differential ultracentrifugation, size exclusion chromatography, and density gradient ultracentrifugation.…”

Section: Characterization Of Evs Molecular Contentmentioning

confidence: 99%

Recent advances in optical label-free characterization of extracellular vesicles

et al. 2022

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Extracellular vesicles (EVs) are complex biological nanoparticles endogenously secreted by all eukaryotic cells. EVs carry a specific molecular cargo of proteins, lipids, and nucleic acids derived from cells of origin and play a significant role in the physiology and pathology of cells, organs, and organisms. Upon release, they may be found in different body fluids that can be easily accessed via noninvasive methodologies. Due to the unique information encoded in their molecular cargo, they may reflect the state of the parent cell and therefore EVs are recognized as a rich source of biomarkers for early diagnostics involving liquid biopsy. However, body fluids contain a mixture of EVs released by different types of healthy and diseased cells, making the detection of the EVs of interest very challenging. Recent research efforts have been focused on the detection and characterization of diagnostically relevant subpopulations of EVs, with emphasis on label-free methods that simplify sample preparation and are free of interfering signals. Therefore, in this paper, we review the recent progress of the label-free optical methods employed for the detection, counting, and morphological and chemical characterization of EVs. We will first briefly discuss the biology and functions of EVs, and then introduce different optical label-free techniques for rapid, precise, and nondestructive characterization of EVs such as nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy, surface plasmon resonance spectroscopy, Raman spectroscopy, and SERS spectroscopy. In the end, we will discuss their applications in the detection of neurodegenerative diseases and cancer and provide an outlook on the future impact and challenges of these technologies to the field of liquid biopsy via EVs.

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Surface enhanced Raman scattering of extracellular vesicles for cancer diagnostics despite isolation dependent lipoprotein contamination

Abstract: Given the emerging diagnostic utility of extracellular vesicles (EVs), it is important to account for non-EV contaminants. Lipoprotein present in EV-enriched isolates may inflate particle counts and decrease sensitivity to...

Cited by 34 publications

References 79 publications

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions

The Promise of Circulating Tumor DNA in Head and Neck Cancer

Recent advances in optical label-free characterization of extracellular vesicles

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