Surface-Enhanced Raman Scattering Based Ligase Detection Reaction

Huh, Yun Suk; Lowe, Adam; Strickland, Aaron D.; Batt, Carl A.; Erickson, David

doi:10.1021/ja807526v

Cited by 82 publications

(65 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The italicized portions of the target sequence are the complementary nucleotides to each capture probe. Details regarding the functionalization of the gold nanoparticles and DNA hybridization procedures can be found in our previous works (26). In short, to immobilize the probes, 300 nM thiolated capture DNA were added to 0.3 nM gold colloid solution in PBS buffer solution for 6 h at room temperature, followed by a 1-h exposure to 30 M carboxy-EG6-undecanethiol to prohibit nonspecific binding.…”

Section: Methodsmentioning

confidence: 99%

“…In a number of recent studies (26)(27)(28), metal colloid aggregation and concentration has been shown to significantly enhance the sensitivity and reproducibility of SERS-based measurements. Chou et al (29) for example recently reported a device that trapped metal nanoparticles at a microchannelnanochannel junction, which has the coupled effect of both increasing the intensity of the surface plasmon field (through multiparticle interactions) and the number of local emitters ultimately yielding an enhanced limit of detection for solution phase ␤-amyloids.…”

Section: Sers-based Detection Of Nucleic Acidsmentioning

confidence: 99%

See 1 more Smart Citation

A method for nanofluidic device prototyping using elastomeric collapse

Park

Huh

Craighead

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

140

118

View full text Add to dashboard Cite

Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques are limited by the complexity of the devices that can be fabricated, which can only create straight or irregular channels normal to the direction of an applied strain. Here, we report a technique for nanofluidic fabrication based on the controlled collapse of microchannel structures. As is demonstrated, this method converts the easy to control vertical dimension of a PDMS mold to the lateral dimension of a nanochannel. We demonstrate here the creation of complex nanochannel structures as small as 60 nm and provide simple design rules for determining the conditions under which nanochannel formation will occur. The applicability of the technique to biomolecular analysis is demonstrated by showing DNA elongation in a nanochannel and a technique for optofluidic surface enhanced Raman detection of nucleic acids.concentrator ͉ nanofluidic channel ͉ single molecule manipulation ͉ surface enhanced raman scattering O f the many reasons why nanofluidic (1-8) systems are of interest, the most well developed applications revolve around sensing, detection, and species handling in single or ''few'' molecule environments. Researchers have recently demonstrated unique bioanalytical capabilities in nanofluidic devices including the ability to elongate single DNA molecules (2), concentrate protein samples by more than four orders of magnitude (9), and efficiently separate both large (3, 10) and small (4) biomolecules. As a result of their technological promise, numerous methods have been developed to fabricate these systems, including electron beam lithography (11, 12), focused ion beam milling (13), interference lithography (14), AFM lithography (15), and nano-imprint lithography (2, 16). The significant advantages of these high-end nanofabrication technologies are their high resolution, reproducibility, and flexibility. Despite these advantages, these methods are somewhat limited when it comes to rapid prototyping of nanofluidic systems. To augment these high-resolution techniques, several groups have developed simpler nanofluidic fabrication in polydimethylsiloxane (PDMS) using lower resolution lithography methods. Huh et al. (17) for example used crack formation in a surface oxide layer to make nanochannels with mechanically tunable widths. Similarly, Chung et al. (18) used wrinkles on an elastomeric PDMS surface that, when bonded to another surface, formed discrete nanochannels. While these approaches greatly simplify the fabrication of nanochannels, the complexity of the devices that can be created is relatively low, in that only straight lines orthogonal to the stretching force can be fabricated.An additional challenge of nanofluidic fabrication with soft materials like PDMS is the relatively low stiffness ...

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Sers-based Detection Of Nucleic Acidsmentioning

confidence: 99%

A method for nanofluidic device prototyping using elastomeric collapse

Park

Huh

Craighead

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

140

118

View full text Add to dashboard Cite

show abstract

“…The ability to rapidly screen for SNPs will have a profound impact on numerous applications, most notably in personalized medicine. Huh et al 153 demonstrated a new approach to SNP detection through the application of SERS to the ligase detection reaction. In order to demonstrate the feasibility of antibody-conjugated GNRs for the highly sensitive targeting and imaging of specific cancer markers expressed on the surface membrane of cancer cells, GNRs with different aspect ratios were synthesized, and their SERS enhancement capability was evaluated.…”

Section: Biological and Biomedicalmentioning

confidence: 99%

Surface-enhanced Raman scattering microfluidic sensor

Wang

2013

RSC Adv.

View full text Add to dashboard Cite

Surface-enhanced Raman scattering (SERS) is a highly sensitive detection technique used to provide information about chemical and biological trace analytes. The confocal Raman microscope makes it possible to monitor small samples in a narrow microfluidic channel, despite the fact that the scattering intensity of the Raman signal is very weak. Recently, microfluidic chip devices coupled with on-chip SERS detection method and their applications to sensitive chemical and biological analyses have attracted significant attention. In this mini-review, highlights of advances in SERS techniques, microfluidic devices and their applications on the biological/environmental analysis of minute analytes within the recent years are provided to illustrate the impact of this rapidly growing area of research.

show abstract

“…With the development of genetic therapy, clinical diagnosis and molecular biology, SNP is regarded as not only a genetic marker in the study of cancer-related drug metabolism or reactivity, but also a fundamental tool in the identification of inherited disease-causing genes (Imyanitov et al, 2004;McCarthy and Hilfiker, 2000;Sidransky, 2002;Sauna, 2007). Therefore, great efforts have been devoted to developing technique methods for screening SNP, such as allele specific oligonucleotide hybridization (Ding et al, 2010;Liu et al, 2005;Liu and Lin, 2007), endonuclease digestion (Gaylord et al, 2005;Li and Liu, 2009), primer extension (Duan et al, 2009a;Litos et al, 2009;Nelson et al, 1996), oligonucleotide ligation (Huh et al, 2009;Lowe et al, 2010;Xue et al, 2009), nonenzymatic ligation (Xu et al, 2001), DNA-specific redox indicators and conjugated mediators (Drummond et al, 2003;Kelley et al, 1999), in combination with fluorescence (FL) (Duan et al, 2007;Guo et al, 2010;Liu et al, 2009;Wang and Liu, 2007), electrochemistry (Kerman et al, 2004;, chemiluminescence (Liu et al, 2006), colorimetry (He et al, 2010a,b;Lee et al, 2010), and mass spectrometry (Mattes and Seitz, 2001) as signal readout.…”

Section: Introductionmentioning

confidence: 99%