Surface Engineering of Quantum Dots for <i>In Vivo</i> Vascular Imaging

Jayagopal, Ashwath; Russ, Patricia K.; Haselton, Frederick R.

doi:10.1021/bc070020r

Cited by 103 publications

(77 citation statements)

References 43 publications

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“…molarity was about 0.227 nM to 227 nM). According to previous reports, the concentration which we tested in our following experiments is similar to the doses that have actually used in in vivo bioimaging (up to hundreds of nanomole) (Ballou et al 2007;Ballou et al 2004;Cai et al 2006;Gao et al 2004;Jayagopal et al 2007;Larson et al 2003;Tada et al 2007) or tested in other in vitro toxicological studies (up to hundreds of nanomole or μg/mL) 20 (Chang et al 2009;Chen et al 2010;Choi et al 2007;Lovric et al 2005;Su et al 2009;Su et al 2010;Tang et al 2008;Wu et al 2010). Then, our cell viability assays (Fig.…”

Section: Discussionmentioning

confidence: 92%

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An in vitro study of vascular endothelial toxicity of CdTe quantum dots

Yan¹,

Zhang²,

Xu³

et al. 2011

Toxicology

113

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Quantum dots (QDs), as novel bioimaging and drug delivery agents, are generally introduced into vascular system by injection, and thus directly exposed to vascular endothelial cells (ECs). However, the adverse effects of QDs on ECs are poorly understood. In this study, employing human umbilical vein ECs (HUVECs), we investigated the potential vascular endothelial toxicity of mercaptosuccinic acid (MSA)-capped CdTe QDs in vitro. In the experiment, water-soluble and pH stable CdTe QDs were synthesized; and the cell viability assays showed that CdTe QDs (0.1-100 mu g/mL) dose-dependently decreased the cell viability of HUVECs, indicating CdTe QDs induced significant endothelial toxicity. The flow cytometric and immunofluorescence results revealed that 10 mu g/mL CdTe QDs elicited significant oxidative stress, mitochondrial network fragmentation as well as disruption of mitochondrial membrane potential (Delta psi(m)); whereas ROS scavenger could protect HUVECs from QDs-induced mitochondrial dysfunction. Moreover, upon 24h exposure to 10 mu g/mL CdTe QDs, the apoptotic HUVECs dramatically increased by 402.01%, accompanied with alternative expression of apoptosis proteins, which were upregulation of Bax, down-regulation of Bcl-2, release of mitochondrial cytochrome c and cleavage of caspase-9/caspase-3. These results suggested that CdTe QDs could not only impair mitochondria but also exert endothelial toxicity through activation of mitochondrial death pathway and induction of endothelial apoptosis. Our results provide strong evidences of the direct toxic effects of QDs on human vascular ECs, and reveal that exposure to QDs is a significant risk for the development of cardiovascular diseases. These results also provide helpful guidance on the future safe use and manipulation of QDs to make them more suitable tools in nanomedicine

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Section: Discussionmentioning

confidence: 92%

“…Lately, QDs were reported to label dermal, adipose, tumor and retinal vasculature (Cai et al 2006;Jayagopal et al 2007;Larson et al 2003). These angiographic techniques provide noninvasive optical access to the vasculature and continuous monitoring of vascular functions.…”

Section: Introductionmentioning

confidence: 99%

An in vitro study of vascular endothelial toxicity of CdTe quantum dots

Yan¹,

Zhang²,

Xu³

et al. 2011

Toxicology

113

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“…Antibody fragments possessing free thiol groups have been bound to free amino groups of quantum dots by means of a heterobifunctional crosslinker (Jayagopal et al 2007), and biotinylated antibodies to streptavidin-modified quantum dots (Dahan et al 2003). More protocols and a comparison of the different approaches have been carried out recently (Xing et al 2007).…”

Section: (Iii) Peptides Proteins Enzymes and Antibodiesmentioning

confidence: 99%

“…As to chemical functional groups, there are a number of colorimetric assays reported that allow for the quantification of amine groups, thiols and others (Ballou et al 2004;Jayagopal et al 2007). Possibly, a chromophore released by the reaction with the target analyte (Zhao et al 2002a;Maus et al 2009) has to be separated from the nanoparticles because of overlapping absorption spectra.…”

Section: (E) Characterizationmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,397

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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“…22,25 Nevertheless, many researchers have observed various nonspecific imaging and passive targeting properties of QDs, namely, the behavior of the QDs but not the functional groups. 26,27 Therefore, to better understand and predict the efficacy and side effects of QDs in vivo, some key questions need to be addressed: Where are these nanocrystals deposited within the tissue and cell?…”

Section: Introductionmentioning

confidence: 99%

In vivo biodistribution and behavior of CdTe/ZnS quantum dots

Zhao¹,

Zhang²,

Qin³

et al. 2017

IJN

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The unique features of quantum dots (QDs) make them desirable fluorescent tags for cell and developmental biology applications that require long-term, multitarget, and highly sensitive imaging. In this work, we imaged fluorescent cadmium telluride/zinc sulfide (CdTe/ZnS) QDs in organs, tissues, and cells, and analyzed the mechanism of their lymphatic uptake and cellular distribution. We observed that the fluorescent CdTe/ZnS QDs were internalized by lymph nodes in four cell lines from different tissue sources. We obtained the fluorescence intensity–QD concentrations curve by quantitative analysis. Our results demonstrate that cells containing QDs can complete mitosis normally and that distribution of QDs was uniform across cell types and involved the vesicular transport system, including the endoplasmic reticulum. This capacity for CdTe/ZnS QD targeting provides insights into the applicability and limitations of fluorescent QDs for imaging biological specimens.

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Surface Engineering of Quantum Dots for In Vivo Vascular Imaging

Cited by 103 publications

References 43 publications

An in vitro study of vascular endothelial toxicity of CdTe quantum dots

An in vitro study of vascular endothelial toxicity of CdTe quantum dots

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

In vivo biodistribution and behavior of CdTe/ZnS quantum dots

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