Surface engineering of inorganic nanoparticles for imaging and therapy

Nam, Jutaek; Won, Nayoun; Bang, Jiwon; Jin, Ho; Park, Joonhyuck; Jung, Sungwook; Jung, Sanghwa; Park, Youngrong; Kim, Sungjee

doi:10.1016/j.addr.2012.08.015

Cited by 306 publications

(199 citation statements)

References 305 publications

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“…The conjugation of biomolecules could impart the NPs with both biocompatibility and desired functionality such as the ability of specific recognition [87,[93][94][95]. The available biomolecules cover from small biomolecules like biotin and folic acid to biomacromolecules like avidin, streptavidin, antibodies, peptides, aptamers and DNA, subjected to the expected target capabilities.…”

Section: Bioconjugationmentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide (Ln 3+ )-doped nanoparticles (NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln 3+ -doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed. INTRODUCTIONSensitive and specific bioassay of trace amount of target analytes is essential for a variety of biomedical applications ranging from pharmaceutical preparation to disease diagnosis and therapy [1][2][3]. Among various bioassay methods, luminescent bioassay has received particular attention because of its high sensitivity and good specificity [4,5]. Conventional luminescent bioassay techniques such as enzyme-linked immunosorbent assay (ELISA), time-resolved (TR) fluoroimmunoassay (TRFIA), Förster resonance energy transfer (FRET) and TR-FRET assays have laid the foundation for many modern clinical applications [6][7][8][9][10][11]. However, these bioassays are impeded by the availability of traditional bioprobes like organic dyes, lanthanide (Ln 3+ ) chelates and quantum dots (QDs). The use of these bioprobes has a number of limitations. Organic dyes commonly possess poor photochemical stability and suffer from serious photobleaching [12]. The applicability of QDs is compromised by photoblinking and high toxic- ity of heavy metal elements like cadmium and selenium, especially for in vivo biosensing [13,14]. Moreover, both organic dyes and QDs may induce high background noise and considerable photodamage to the biological samples under ultraviolet (UV) excitation, which deteriorates their detection sensitivity for bioassays [15,16]. Although such background noise can be suppressed by the technique of TR photoluminescence (PL) through the use of the longlived luminescence of Ln 3+ -chelates, the poor photochemical stability, long-term toxicity and high cost of Ln 3+ -chelates remain a major issue [17]. These concerns fuel a crucial demand for the development of a new generation of luminescent bioprobes to circumvent the limitations of traditional ones.Recently, with the explosion of nanoscience and nanotechnology, there is a growing dedication towards the development of diverse luminescent nanoprobes for biodetection ...

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Section: Bioconjugationmentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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“…22,25 Nevertheless, many researchers have observed various nonspecific imaging and passive targeting properties of QDs, namely, the behavior of the QDs but not the functional groups. 26,27 Therefore, to better understand and predict the efficacy and side effects of QDs in vivo, some key questions need to be addressed: Where are these nanocrystals deposited within the tissue and cell?…”

Section: Introductionmentioning

confidence: 99%

“…19 Recently, we reported the development of a novel biosensor using CdTe/ZnS QD-conjugated monoclonal antibody as a highly sensitive probe. 20 The passive targeting of QDs also allows their application in drug delivery 21,22 and imaging. 21,23,24 Despite the significant attention given to CdTe/ZnS QDs, however, studies on the in vivo biodistribution and the behavior of CdTe/ZnS QDs are still relatively limited and nonsystematic.…”

Section: Introductionmentioning

confidence: 99%

In vivo biodistribution and behavior of CdTe/ZnS quantum dots

Zhao¹,

Zhang²,

Qin³

et al. 2017

IJN

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The unique features of quantum dots (QDs) make them desirable fluorescent tags for cell and developmental biology applications that require long-term, multitarget, and highly sensitive imaging. In this work, we imaged fluorescent cadmium telluride/zinc sulfide (CdTe/ZnS) QDs in organs, tissues, and cells, and analyzed the mechanism of their lymphatic uptake and cellular distribution. We observed that the fluorescent CdTe/ZnS QDs were internalized by lymph nodes in four cell lines from different tissue sources. We obtained the fluorescence intensity–QD concentrations curve by quantitative analysis. Our results demonstrate that cells containing QDs can complete mitosis normally and that distribution of QDs was uniform across cell types and involved the vesicular transport system, including the endoplasmic reticulum. This capacity for CdTe/ZnS QD targeting provides insights into the applicability and limitations of fluorescent QDs for imaging biological specimens.

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“…For example, SLN QDs with this turn-on function can be used for simultaneous mapping and diagnosis of the malignancy. Sophisticated control of the QD surface is a prerequisite for the development of such a turn-on function (6 ). Innovations in tomographical optical imaging techniques that can exploit multiply scattered photons from deep tissues are also required to achieve deep and sensitive NIR QD imaging for clinical applications.…”

mentioning

confidence: 99%