Surface Engineering of Auxetic Scaffolds for Neural and Vascular Differentiation from Human Pluripotent Stem Cells

Chen, Xingchi; Liu, Chang; Wadsworth, Matthew; Zeng, Eric Z.; Driscoll, Tristan P.; Zeng, Changchun; Li, Yan

doi:10.1002/adhm.202202511

Cited by 6 publications

(1 citation statement)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their findings indicated that auxetic scaffolds notably enhanced PSC differentiation under neural induction conditions [ 25 ]. Collectively, these studies affirm that the unique biophysical properties of auxetic materials can enhance structural capability and foster cell differentiation under specific conditions, offering strategies for designing tissue engineering scaffolds to repair myocardial function [ 26 ].…”

Section: Introductionmentioning

confidence: 90%

Composite patch with negative Poisson's ratio mimicking cardiac mechanical properties: Design, experiment and simulation

Dong,

Ren,

Jia

et al. 2024

Materials Today Bio

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 90%

Composite patch with negative Poisson's ratio mimicking cardiac mechanical properties: Design, experiment and simulation

Dong,

Ren,

Jia

et al. 2024

Materials Today Bio

View full text Add to dashboard Cite

Bio‐Metamaterials for Mechano‐Regulation of Mesenchymal Stem Cells

Munding

Fladung

Chen

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

Cell behaviors significantly depend on the elastic properties of the microenvironments, which are distinct from commonly used polymer‐based substrates. Artificial elastic materials called metamaterials offer large freedom to adjust their effective elastic properties as experienced by cells, provided (i) the metamaterial unit cell is sufficiently small compared to the biological cell size and (ii) the metamaterial is sufficiently soft to deform by the active cell contraction. Thus, metamaterials targeting bio‐applications (bio‐metamaterials) appear as a promising path toward the mechanical control of stem cells. Herein, human mesenchymal stem cells (hMSCs) are cultured on three different types of planar periodic elastic metamaterials. To fulfill the above two key requirements, microstructured bio‐metamaterials have been designed and manufactured based on a silicon elastomer‐like photoresist and two‐photon laser printing. In addition to the conventional morphometric and immunocytochemical analysis, the traction force that hMSCs exert on metamaterials are inferred by converting the measured displacement‐vector fields into force‐vector fields. The differential responses of hMSCs, both on the cellular level and the sub‐cellular level, correlate with the calculated effective elastic properties of the bio‐metamaterials, suggesting the potential of bio‐metamaterials toward mechanical regulation of cell behaviors by the arrangement of unit cells.

show abstract

Viscoelasticity of Hyaluronic Acid Hydrogels Regulates Human Pluripotent Stem Cell‐derived Spinal Cord Organoid Patterning and Vascularization

Chen,

Liu,

McDaniel

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Recently, it has been recognized that natural extracellular matrix (ECM) and tissues are viscoelastic, while only elastic properties have been investigated in the past. How the viscoelastic matrix regulates stem cell patterning is critical for cell‐ECM mechano‐transduction. Here, this study fabricated different methacrylated hyaluronic acid (HA) hydrogels using covalent cross–linking, consisting of two gels with similar elasticity (stiffness) but different viscoelasticity, and two gels with similar viscoelasticity but different elasticity (stiffness). Meanwhile, a second set of dual network hydrogels are fabricated containing both covalent and coordinated cross–links. Human spinal cord organoid (hSCO) patterning in HA hydrogels and co‐culture with isogenic human blood vessel organoids (hBVOs) are investigated. The viscoelastic hydrogels promote regional hSCO patterning compared to the elastic hydrogels. More viscoelastic hydrogels can promote dorsal marker expression, while softer hydrogels result in higher interneuron marker expression. The effects of viscoelastic properties of the hydrogels become more dominant than the stiffness effects in the co‐culture of hSCOs and hBVOs. In addition, more viscoelastic hydrogels can lead to more Yes‐associated protein nuclear translocation, revealing the mechanism of cell‐ECM mechano‐transduction. This research provides insights into viscoelastic behaviors of the hydrogels during human organoid patterning with ECM‐mimicking in vitro microenvironments for applications in regenerative medicine.

show abstract

Surface Engineering of Auxetic Scaffolds for Neural and Vascular Differentiation from Human Pluripotent Stem Cells

Cited by 6 publications

References 74 publications

Composite patch with negative Poisson's ratio mimicking cardiac mechanical properties: Design, experiment and simulation

Composite patch with negative Poisson's ratio mimicking cardiac mechanical properties: Design, experiment and simulation

Bio‐Metamaterials for Mechano‐Regulation of Mesenchymal Stem Cells

Viscoelasticity of Hyaluronic Acid Hydrogels Regulates Human Pluripotent Stem Cell‐derived Spinal Cord Organoid Patterning and Vascularization

Contact Info

Product

Resources

About