Surface-engineered CuFeS2/Camptothecin nanoassembly with enhanced chemodynamic therapy via GSH depletion for synergistic photo/chemotherapy of cancer

Preclinical Assessment of Enhanced Chemodynamic Therapy by an FeMnOx-Based Nanocarrier: Tumor-Microenvironment-Mediated Fenton Reaction and ROS-Induced Chemotherapeutic for Boosted Antitumor Activity

“…The powerful intracellular antioxidant GSH absorbs ROS generated within the cell and externally, increasing hypoxia and reducing the efficacy of ROS-based treatments. ,, In a somewhat acidic environment, the ability of FeMnO x NPs to deactivate the GSH reagent was examined by using a DTNB probe. The GSH solution and Fe 3+ /Mn 4+ ions reacted to release GSSG (Fe 3+ /Mn 4+ + GSH → Fe 2+ /Mn 2+ + GSSG) (Figure a), which was subsequently associated with DTNB to generate a yellow TNB intermediate with a distinctive absorption spectrum at 412 nm (Figure a) .…”

“…The capability of FeMnO x NPs to reduce GSH concentration was quantified using Ellman’s confirmatory probe. , The existence of Fe 3+ /Mn 4+ ions allows them to react with GSH and transform into GSSG, intermediates that produce an absorbance peak at nearly 412 nm. A solution consisting of 50 μL of GSH (1 mM) and 250 μL of different FeMnO x NPs was prepared in PBS (5.0) to evaluate this concept.…”

“…In the meantime, standard groups comprising 0.2 μL of DNTB reagent and 100 μL of GSH solution as standard (0, 20, 40, 60, 80, and 100 μM) were fixed. After 5 min, a Bio-Tek microplate absorption spectrum was quantified for all samples at 412 nm. ,, Amount .25em of .25em GSH .25em after .25em treatment ( % ) = false( OD .25em NPs − OD .25em blank false) false( OD .25em control − OD .25em blank false) × 100 % where OD blank is the optical density of the blank, OD control is the optical density of untreated cells, and OD NPs is the optical density of FeMnO x NP treated cells.…”

“…After 5 min, a Bio-Tek microplate absorption spectrum was quantified for all samples at 412 nm. 24,28,30 Amount of GSH after treatment(%) (OD NPs OD blank)…”

Heterogeneous-Driven Glutathione Oxidation: Defining the Catalytic Role of Chalcopyrite Nanoparticles

“…Only a scarce number of mixed Cu–Fe sulfide-based nanostructured analogues have reported GSH consumption. , In the case of monometallic sulfides, CuS , and FeS 2 were also reported to oxidize GSH. Tang et al recently reported the synthesis of CuS NPs with the capability to oxidize GSH, but they attributed this oxidation to the Cu 2+ released as a consequence of a combination of the lower pH in tumors and near-infrared (NIR) irradiation.…”