Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Abstract

Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It was experimentally found that nitrosyl tetrafluoroborate pretreatment and proper structures of molecules are essential to the effective surface functionalization of SPIONs. Nonspecific binding was found to be significantly reduced by BSA surface functionalized hydrophobic SPIONs (BSA·SPIONs). The BSA·SPIONs were monodispersed with an average size of approximately 18.0 nm and stable in a wide pH range and various ionic strengths even after 7 days of storage. The longitudinal and transverse proton relaxation rate (r 1 , r 2 ) values of the BSA·SPIONs were determined to be 11.6 and 154.2 s −1 per mM of Fe 3+ respectively. The r 2 /r 1 ratio of 13.3 ensured its application as the T 2 -weighted magnetic resonance imaging contrast agents. When conjugated with near-infrared fluorescent dye and monoclonal antibody, the dye BSA·SPION-monoclonal antibody bioconjugates showed excellent targeting capability with minimal nonspecific binding in the bimodal imaging of pancreatic cancer cells. The experimental approach is facile, environmentally benign, and straightforward, which presents great promise in early cancer diagnosis.

Synthesis and characterization of silica‐coated superparamagnetic iron oxide nanoparticles and interaction with pancreatic cancer cells

“…The cytotoxicity test of NPs on PANC‐1 cells was performed by different methods . First, the LC 50 results show that after 48 hours of exposure the toxicity for both SiO 2 ‐Fe 3 O 4 NPs and Fe 3 O 4 NPs (around 150 μg/mL) was within a biologically accepted standard deviation for both materials (see Figure ), despite the SiO 2 ‐Fe 3 O 4 NPs show a higher variance in the low concentrations, which is comparable with previous literature .…”

Synthesis of Ultrathin MnS Shell on ZnS: Mn Nanorods by One‐Step Coating and Doping for MRI and Fluorescent Imaging

“…To prepare these bioprobes, the obtained core-shell NRs were surface-modifi ed using 1,2-ethanedithiol and bovine serum albumin (BSA) based on previously reported methods. [ 61 ] Curve III in Figure 6 [ 59,60 ] After being transferred, the obtained colloidal aqueous solution was stable with very few precipitation for 3 days ( Figure S1, Supporting Information).…”

An “imaging-biopsy” strategy for colorectal tumor reconfirmation by multipurpose paramagnetic quantum dots

“…BSA and its derivatives have numerous biochemical applications that include blocking agents in enzyme-linked immunosorbent assay [30], stabilizers in enzymes processing [31], ligands in surface engineering [24,32] and nanocarriers [33e35]. In this study, BSA has two roles, namely the ligands for surface engineering of hydrophobic QDs and nanocarriers for linking Gd DTPA and antibodies.…”

“…In this study, BSA has two roles, namely the ligands for surface engineering of hydrophobic QDs and nanocarriers for linking Gd DTPA and antibodies. Practically, BSA, as a multivalent ligand with abundant free carboxyl, amino and disulfide bonds, has been successfully used in our previous work for surface engineering of nanopaticles [24,32]. In this study, for multipurpose applications, BSA is employed with MRI moiety beforehand, and utilized for water-solubilization of hydrophobic QDs.…”