Surface coating mediates the toxicity of polymeric nanoparticles towards human-like macrophages

Grabowski, Nadège; Hillaireau, Hervé; Vergnaud-Gauduchon, Juliette; Tsapis, Nicolas; Pallardy, Marc; Kerdine-Römer, Saadia; Fattal, Elias

doi:10.1016/j.ijpharm.2014.11.042

Cited by 108 publications

(76 citation statements)

References 21 publications

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“…For instance, cytotoxicity was found to be induced by chitosan on macrophage when used as poly(lactide-co-glycolic) (PLGA) nanoparticle stabilizer [49] or in hybrid hydrogels, where it induces TNF-a and NO production by RAW 264.7 macrophages [50]. In our previous report, in chitosan-based matrix did not show any sign of toxicity on osteoblastic lines [39].…”

Section: Cytotoxicity Evaluationmentioning

confidence: 98%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

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a b s t r a c tIn recent years, there has been an increasing interest in the design of biomaterials for cartilage tissue engineering. This type of materials must meet several requirements. In this study, we apply ultrasound to prepare a compatibilized blend of polyelectrolyte complexes (PEC) based on carboxymethyl cellulose (CMC) and chitosan (CHI), in order to improve stability and mechanical properties through the interpolymer macroradicals coupling produced by sonochemical reaction. We study the kinetic of the sonochemical degradation of each component in order to optimize the experimental conditions for PEC compatibilization. Scaffolds obtained applying this methodology and scaffolds without ultrasound processing were prepared and their morphology (by scanning electron microscopy), polyelectrolyte interactions (by FTIR), stability and mechanical properties were analyzed. The swelling kinetics was studied and interpreted based on the structural differences between the two kinds of scaffolds. In addition we evaluate the possible in vitro cytotoxicity of the scaffolds using macrophage cells in culture. Our results demonstrate that the ultrasound is a very efficient methodology to compatibilize PEC, exhibiting improved properties compared with the simple mixture of the two polysaccharides. The test with murine macrophage RAW 264.7 cells showed no evince of cytotoxicity, suggesting that PEC biomaterials obtained under ultrasound conditions could be useful in the cartilage tissue engineering field.

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Section: Cytotoxicity Evaluationmentioning

confidence: 98%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

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“…[56][57][58] The use of FITC-labeled BSA allowed us to detect and localize the protein either free or encapsulated into PLGA NPs and monitor its uptake and delivery process in THP-1 MDM cells. Flow cytometry analysis demonstrated that BSA-loaded PLGA NPs are easily taken up by human MDMs and that BSA delivery in this cell model is increased fivefold when the protein is encapsulated into PLGA NPs.…”

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confidence: 99%

Preparation, characterization, and safety evaluation of poly(lactide-co-glycolide) nanoparticles for protein delivery into macrophages

Guedj¹,

Kell²,

Barnes³

et al. 2015

IJN

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Following infection, HIV establishes reservoirs within tissues that are inaccessible to optimal levels of antiviral drugs or within cells where HIV lies latent, thus escaping the action of anti-HIV drugs. Macrophages are a persistent reservoir for HIV and may contribute to the rebound viremia observed after antiretroviral treatment is stopped. In this study, we further investigate the potential of poly(lactic- co -glycolic) acid (PLGA)-based nanocarriers as a new strategy to enhance penetration of therapeutic molecules into macrophages. We have prepared stable PLGA nanoparticles (NPs) and evaluated their capacity to transport an active molecule into the human monocyte/macrophage cell line THP-1 using bovine serum albumin (BSA) as a proof-of-concept compound. Intracellular localization of fluorescent BSA molecules encapsulated into PLGA NPs was monitored in live cells using confocal microscopy, and cellular uptake was quantified by flow cytometry. In vitro and in vivo toxicological studies were performed to further determine the safety profile of PLGA NPs including inflammatory effects. The size of the PLGA NPs carrying BSA (PLGA-BSA) in culture medium containing 10% serum was ~126 nm in diameter, and they were negatively charged at their surface (zeta potential =−5.6 mV). Our confocal microscopy studies and flow cytometry data showed that these PLGA-BSA NPs are rapidly and efficiently taken up by THP-1 monocyte-derived macrophages (MDMs) at low doses. We found that PLGA-BSA NPs increased cellular uptake and internalization of the protein in vitro. PLGA NPs were not cytotoxic for THP-1 MDM cells, did not modulate neutrophil apoptosis in vitro, and did not show inflammatory effect in vivo in the murine air pouch model of acute inflammation. In contrast to BSA alone, BSA encapsulated into PLGA NPs increased leukocyte infiltration in vivo, suggesting the in vivo enhanced delivery and protection of the protein by the polymer nanocarrier. We demonstrated that PLGA-based nanopolymer carriers are good candidates to efficiently and safely enhance the transport of active molecules into human MDMs. In addition, we further investigated their inflammatory profile and showed that PLGA NPs have low inflammatory effects in vitro and in vivo. Thus, PLGA nanocarriers are promising as a drug delivery strategy in macrophages for prevention and eradication of intracellular pathogens such as HIV and Mycobacterium tuberculosis .

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“…PLGA and its derivatives it is known that their drug release is based on the dissolution rate of its surface coating polymeric material PLGA [37]. Nevertheless, it was demonstrated in macrophages that the surface coating of these polymeric nanoparticles may be linked to induced toxicity by increasing oxidative stress levels [38].…”

Section: Toxicity Of Nanoparticles Due To Dissolutionmentioning

confidence: 99%

Bioaccumulation and Toxic Profiling of Nanostructured Particles and Materials

Dinda¹

2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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Use of nanotechnological based formulations and nanomaterials are increasing day-byday in wide range covering a broad typology of applications, from design and development of targeted drug delivery systems, manufacturing of pesticides, domestic appliances, textiles, to bioremediation engineering. There are therefore concerns about the environmental risks or bioaccumulation-related issues that may arise particularly resulting from the application of drug-loaded nanocarriers or efect of pesticides that reach the natural ecosystems. This is a major threat in the present era and needs to be balanced against their undoubted beneits to human society. The assessment of the physical and chemical properties of nanoparticles and nanomaterials inluencing their toxic manifestation due to accumulation in human or in animal organs is still poorly investigated. This chapter reviews the possibilities of bioaccumulation of diferent nanoscale particles and materials, their potential acute and subacute toxicological proile and their identiication and characterization in diferent organs and tissues of vertebrates.

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Surface coating mediates the toxicity of polymeric nanoparticles towards human-like macrophages

Cited by 108 publications

References 21 publications

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Preparation, characterization, and safety evaluation of poly(lactide-co-glycolide) nanoparticles for protein delivery into macrophages

Bioaccumulation and Toxic Profiling of Nanostructured Particles and Materials

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