Surface chemistry from wettability and charge for the control of mesenchymal stem cell fate through self-assembled monolayers

Li, Hao; Fu, Xiaoling; Li, Tianjie; Zhao, Naru; Shi, Xuetao; Cui, Fuzhai; Du, Chang; Wang, Yingjun

doi:10.1016/j.colsurfb.2016.09.027

Cited by 73 publications

(55 citation statements)

References 32 publications

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“…CL11 modified substrates resulted in a controlled deposition of dense roughened fibronectin matrices, both in proteinaceous and cell containing environments, a major contributing factor to enhanced levels of hMSC adhesion and induced osteogenesis (Figs. ).…”

Section: Discussionmentioning

confidence: 99%

“…The development and optimization of technologies that can translate into a manufacturing process to meet these design criteria is essential if the potential of biomaterials in regenerative medicines is to be achieved . Silane modification offers a way to enrich a surface with a specific chemical group and is a process that can be applied to an array of base substrates, therefore, it is a process that offers significant potential in biomaterials research, especially in inducing osteogenesis in mesenchymal stem cells . To fulfil its potential as a cost‐effective surface modification tool, the resultant self assembled monolayer (SAM) must have the ability to produce a homogenous cellular response across the entirety of the area of interest.…”

Section: Introductionmentioning

confidence: 99%

“…We, and others, have previously reported on the potential of silane modifications as a mechanism for enriching the surfaces of silicon, gold, glass, PLGA and PCL with –CH 3 , –NH 2 , –OH, and –COOH and the resultant variable effect on stem cell adhesion and differentiation . Within these studies the effect of varying the chain length for a specific chemical moiety was not addressed, nor where the resulting surfaces considered at the submicron scale.…”

Section: Introductionmentioning

confidence: 99%

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The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Chen

Hunt

Fawcett

et al. 2018

J Biomedical Materials Res

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Silane modification has been proposed as a powerful biomaterial surface modification tool. This is the first comprehensive investigation into the effect of silane chain length on the resultant properties of -NH silane monolayers and the associated osteoinductive properties of the surface. A range of -NH presenting silanes, chain length 3-11, were introduced to glass coverslips and characterized using water contact angles, atomic force microscopy, X-ray photoelectron spectroscopy, and Ninhydrin assays. The ability of the variation in chain length to form a homogenous layer across the entirety of the surfaces was also assessed. The osteoinductive potential of the resultant surfaces was evaluated by real-time polymerase chain reaction, immunocytochemistry, and von Kossa staining. Control of surface chemistry and topography was directly associated with changes in chain length. This resulted in the identification of a specific, chain length 11 (CL11) which significantly increased the osteoinductive properties of the modified materials. Only CL11 surfaces had a highly regular nano-topography/roughness which resulted in the formation of an appetite-like layer on the surface that induced a significantly enhanced osteoinductive response (increased expression of osteocalcin, CBFA1, sclerostin, and the production of a calcified matrix) across the entirety of the surface. © 2018 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1862-1877, 2018.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Chen

Hunt

Fawcett

et al. 2018

J Biomedical Materials Res

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“…The characteristic functional groups of the natural polymers used here -hydroxyl (-OH) for cellulose and amine (-NH2) for silk -have been widely studied, with their impact on MSC behaviour characterised [62]- [65]. Both -OH and -NH2 groups encourage MSC adhesion and proliferation through the promotion of cell surface integrins [64], with the latter group demonstrating a higher affinity [62]. Material surface chemistry can also influence the conformation of adsorbed proteins.…”

Section: Human Mesenchymal Stem Cell Viability On Composite Nanofibresmentioning

confidence: 99%

Electrospun Cellulose-Silk Composite Nanofibres Direct Mesenchymal Stem Cell Chondrogenesis in the Absence of Biological Stimulation

Begum

Perriman

et al. 2018

Preprint

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Smart biomaterials with an inherent stimulating capacity that elicit specific behaviours in lieu of biological prompts would prove advantageous for regenerative medicine applications.Specific blends of the natural polymers cellulose and silk cast as films can drive the chondrogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) upon in vitro culture. However, the true potential of such biomaterials for cartilage tissue engineering can be realised upon its three-dimensional fabrication. In this work we employ an electrospinning technique to model the in vivo nanofibrous extracellular matrix (ECM).Cellulose and silk polymers at a mass ratio of 75:25 were regenerated using a trifluoroacetic acid and acetic acid cosolvent system. This natural polymer composite was directly electrospun for the first time, into nanofibers without post-spun treatment. The presence and size of fibre beading was influenced by environmental humidity. The regenerated composite retained the key chemical functionalities of its respective components. Biocompatibility of the natural polymer composite with hMSCs was demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, was confirmed. This physical chondrogenic stimulation was countered biochemically using fibroblast growth factor-2 (FGF-2), a growth factor used to enhance the proliferation of hMSCs. The newly fabricated scaffold provides the foundation for designing a robust, selfinductive, and cost-effective biomimetic biomaterial for cartilage tissue engineering.

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“…The resultant films, denoted as self-assembled monolayers (SAMs), offer great potential for use in a number of applications such as corrosion inhibition [4][5][6][7][8], lithographic patterning [9][10], biomaterials fabrication [11][12][13][14][15][16][17], and microelectronic device manufacturing [18][19][20][21]. In particular, alkanethiolate-based monolayers on gold substrates are the most studied system due to their reproducible ordered structures and relatively simple preparation procedures [22,23].…”

Section: Introductionmentioning

confidence: 99%

Unsymmetrical Spiroalkanedithiols Having Mixed Fluorinated and Alkyl Tailgroups of Varying Length: Film Structure and Interfacial Properties

Chinwangso

Hill

Marquez

et al. 2018

Preprint

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Abstract:A custom-designed series of unsymmetrical spiroalkanedithiols having tailgroups comprised of a terminally fluorinated chain and a hydrocarbon chain of varying lengths were synthesized and used to prepare self-assembled monolayers (SAMs) on gold substrates. The specific structure of the adsorbates was of the formwhere n = 7, 9, and 12 (designated as F8H10-C10, F8H10-C12, and F8H10-C18, respectively).The influence of the length of the hydrocarbon chain in the bidentate dithiol on the structure and interfacial properties of the monolayer was explored. A structurally analogous partially fluorinated monodentate alkanethiol and the corresponding normal alkanethiols were used to generate appropriate SAMs as reference systems. Ellipsometric thickness measurements showed an unexpectedly low film thickness for the SAMs derived from the bidentate adsorbates, possibly due to disruptions in interchain packing caused by the fluorocarbon chains (i.e., phase-incompatible fluorocarbon-hydrocarbon interactions), ultimately giving rise to loosely packed and disordered films. Analysis by X-ray photoelectron spectroscopy (XPS) were also consistent with a model in which the films were loosely packed; additionally, the XPS spectra confirmed the attachment of the sulfur headgroups of the bidentate adsorbates onto the gold substrates. Studies of the SAMs by polarization modulation-infrared reflection-adsorption spectroscopy (PM-IRRAS) suggested that as the length of the hydrocarbon chain in the adsorbates was extended, a more ordered surface was achieved by reducing the tilt of the fluorocarbon segment. The wettability data indicated that the adsorbates with longer alkyl chains were less wettable than those with shorter alkyl chains, likely due to an increase in interchain van der Waals forces in the former.Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted:

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Surface chemistry from wettability and charge for the control of mesenchymal stem cell fate through self-assembled monolayers

Cited by 73 publications

References 32 publications

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

The optimization and production of stable homogeneous amine enriched surfaces with characterized nanotopographical properties for enhanced osteoinduction of mesenchymal stem cells

Electrospun Cellulose-Silk Composite Nanofibres Direct Mesenchymal Stem Cell Chondrogenesis in the Absence of Biological Stimulation

Unsymmetrical Spiroalkanedithiols Having Mixed Fluorinated and Alkyl Tailgroups of Varying Length: Film Structure and Interfacial Properties

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