Surface characterization of polysaccharide scaffolds by inverse gas chromatography regarding application in tissue engineering

Abstract

Scaffolds are of paramount importance in tissue engineering, providing support for cell attachment and allowing proliferation or differentiation, and production of extracellular matrix. Their surface properties are of major interest since in great part, they determine the biological response to the scaffold. In the present work, three chitosan‐based polyelectrolytes scaffolds, namely, 1:1 chitosan‐alginate (Chit‐Alg), chitosan‐pectin (Chit‐Pect), and chitosan‐carboxymethyl cellulose (Chit‐CMC), were studied with regard to their surface properties by inverse gas chromatography. The dispersive component of the surface energy, a measure of the ability of a material to establish Lifshitz Van der Waals interactions, was 47.8 mJ·m−2 for Chit‐Alg, 36.9 mJ·m−2 for Chit‐Pect, and 38.5 mJ·m−2 for Chit‐CMC (at 40°C). All the scaffolds surfaces had an amphoteric character, however, with an apparent prevalence of Lewis acidity over the Lewis basicity. These results have been interpreted in terms of the influence of the different substituent groups in the polysaccharides backbone. The porosities of the Chit‐Alg, Chit‐Pect, and Chit‐CMC scaffolds, assessed by mercury intrusion porosimetry, were found to be of 98%, 96%, and 84%, respectively.

Thermal Surface Properties, London Dispersive and Polar Surface Energy of Graphene and Carbon Materials Using Inverse Gas Chromatography at Infinite Dilution

“…The lack of information and the different gaps encountered in this research area guided the objective of our study for accurate determination of the surface properties of graphene and carbon materials, which are required to obtain real guidance for the design and manufacturing of graphene-based biomaterials, medical instruments, structural composites, electronics, and renewable energy devices [ 20 ]. The present work is thus devoted to determination of the thermal surface properties, the London dispersive and polar acid-base surface energies, and the Lewis acid-base parameters of graphene and carbon materials as a function of the temperature by using the IGC technique [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ] at infinite dilution and applyi...…”

The Effect of Temperature on the London Dispersive and Lewis Acid-Base Surface Energies of Polymethyl Methacrylate Adsorbed on Silica by Inverse Gas Chromatography

“…The estimation of the London dispersive and acid-base surface energies of solids, oxides, and polymers [1][2][3][4][5][6][7][8][9][10][11][12][13][14] is of crucial importance in many industrial processes, such as adhesion, paints, coatings, corrosion, chemical reactions, adsorption, and catalysis. The most used technique to determine these surface parameters was inverse gas chromatography (IGC) at infinite dilution.…”

Characterization of surface properties of chitosan/bentonite composites beads by inverse gas chromatography

“…It is well-established and inexpensive technique for the determination of the influence on solid surface of factors such as milling [22,23], humidity [24], and chemical treatments [25]. The IGC tool has already been implemented in the analysis of solids such as clay [26,27], biopolymers [28,29], bionanocomposites [30][31][32] and composites [33].…”