Surface changes associated with ram sperm cryopreservation revealed by counter-current distribution in an aqueous two-phase system effect of different cryoprotectants

Ollero, Mario; Blanco, Teresa; Lopez-Perez, M. J.; Pérez, José Álvaro Cebrián

doi:10.1016/0378-4347(95)00461-0

Cited by 24 publications

(22 citation statements)

References 31 publications

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“…The motility and acrosomal integrity of spermatozoa submitted to a cold shock (5 8C) were preserved by phosphatidylcholine:cholesterol liposomes (Holt & North 1988). The cryoprotective properties of cholesterol and phosphatidylcholine were confirmed, when added as single agents in the medium, with respect to cell membrane integrity of frozen-thawed sperm cells (Ollero et al 1996). Boar spermatozoa incubated with liposomes made from a complex mixture of selected phospholipids, prior to the freezing-thawing process, showed better motility and viability than sperm incubated with liposomes made from fresh sperm head plasma membranes.…”

Section: Sperm Cryopreservationmentioning

confidence: 88%

Epididymosomes, prostasomes, and liposomes: their roles in mammalian male reproductive physiology

Sullivan¹,

Saez²

2013

Reproduction

247

215

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Mammalian spermatozoa are unique cells in many ways, and the acquisition of their main function, i.e. fertilization capacity, is a multistep process starting in the male gonad and ending near the female egg for the few cells reaching this point. Owing to the unique character of this cell, the molecular pathways necessary to achieve its maturation also show some specific characteristics. One of the most striking specificities of the spermatozoon is that its DNA is highly compacted after the replacement of histones by protamines, making the classical processes of transcription and translation impossible. The sperm cells are thus totally dependent on their extracellular environment for their protection against oxidative stress, for example, or for the molecular changes occurring during the transit of the epididymis; the first organ in which post-testicular maturation takes place. The molecular mechanisms underlying sperm maturation are still largely unknown, but it has been shown in the past three decades that extracellular vesicles secreted by the male reproductive tract are involved in this process. This review will examine the roles played by two types of naturally occurring extracellular vesicles, epididymosomes and prostasomes, secreted by the epididymis and the prostate respectively. We will also describe how the use of artificial vesicles, liposomes, contributed to the study of male reproductive physiology.Reproduction (2013) 146 R21-R35

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Section: Sperm Cryopreservationmentioning

confidence: 88%

Epididymosomes, prostasomes, and liposomes: their roles in mammalian male reproductive physiology

Sullivan¹,

Saez²

2013

Reproduction

247

215

View full text Add to dashboard Cite

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“…, 1998). Freezing damage also affects sperm heterogeneity, which is dramatically diminished (Watson, 1995; Ollero et al. , 1996).…”

Section: Discussionmentioning

confidence: 99%

Morphometric changes in boar spermatozoa induced by cryopreservation

et al. 2008

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Computer-assisted sperm morphometry analysis was used to determine the effects of cryopreservation on boar sperm head and midpiece morphometry. Sperm-rich fractions were collected from five mature boars. Three microscope slides were prepared from single extended sperm samples prior freezing and post-thawing. All slides were stained with Hemacolor, and 250 sperm images were obtained from each slide. The sperm head dimensions for length, width, area, perimeter and four shape factors and sperm-midpiece dimensions for area, width, angle and distance were determined in each spermatozoa. The effects of sperm freezing on sperm dimensions within and among boars were determined. A previous discriminant analysis of the results was able to correctly classify a 78.3 and 82% of fresh and frozen-thawed spermatozoa respectively. Sperm heads were significantly smaller in cryopreserved spermatozoa than in the companion extended samples for length, width, area and perimeter. Sperm midpieces were also significantly smaller in cryopreserved spermatozoa for width and area. The highest changes in morphometric dimensions after the freeze-thawing process were found in the midpiece of spermatozoa. The variability of morphometric measurements only was significantly different between fresh and thawed samples for head rugosity and midpiece area. The effects of cryopreservation on morphometric parameters were similar in the boars, which allow us to conclude that cryopreservation process does not have a different effect in each individual boar. In summary, morphometric changes associated with the cryopreservation process on boar spermatozoa do not apparently depends on an effect at individual level.

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“…It could reverse the functional consequences of LPO as assessed by sperm-oocyte fusion (15). When α-tocopherol was added during sperm freezing, sperm viability was found to improve in human (32), ram (33), and bovine sperm (34). It stabilized sperm membrane integrity and suppressed LPO during sperm capacitation in the bull (35).…”

Section: Discussionmentioning

confidence: 99%

Effects of α-Tocopherol on Cadmium-Induced Toxicity in Rat Testis and Spermatogenesis

et al. 2006

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Cadmium is known to exert toxic effects on multiple organs, including the testes. To determine if α-Tocopherol, an antioxidant, could protect testicular tissues and spermatogenesis from the toxic effects of cadmium, six-week old male Sprague-Dawley rats were randomized to receive cadmium at doses of 0 (control), 1, 2, 4 or 8 mg/kg by the intraperitoneal route (Group A) or α-tocopherol for 5 days before being challenged with cadmium (Group B) in an identical dose-dependent manner. When both groups received cadmium at 1 mg/kg, there were no changes in testicular histology relative to controls. When Group A received cadmium at 2 mg/kg, undifferentiated spermatids and dead Sertoli cells increased in the seminiferous tubules while interstitial cells decreased and inflammatory cells increased in the interstitial tissues. On flow cytometric analysis, the numbers of elongated spermatids (M1) and round spermatids (M2) decreased while 2c stage cells (M3, diploid) increased. In contrast, when Group B received cadmium at 2 mg/kg, the histological insults were reduced and the distribution of the germ cell population remained comparable to controls. However, α-tocopherol had no protective effects with higher cadmium doses of 4 and 8 mg/kg. These findings indicate that α-tocopherol treatment can protect testicular tissue and preserve spermatogenesis from the detrimental effects of cadmium but its effectiveness is dependent on the dose of cadmium exposed.

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Surface changes associated with ram sperm cryopreservation revealed by counter-current distribution in an aqueous two-phase system effect of different cryoprotectants

Cited by 24 publications

References 31 publications

Epididymosomes, prostasomes, and liposomes: their roles in mammalian male reproductive physiology

Epididymosomes, prostasomes, and liposomes: their roles in mammalian male reproductive physiology

Morphometric changes in boar spermatozoa induced by cryopreservation

Effects of α-Tocopherol on Cadmium-Induced Toxicity in Rat Testis and Spermatogenesis

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