Surface attachment of horseradish peroxidase to nylon modified by plasma‐immersion ion implantation

Kondyurin, Alexey; Nosworthy, Neil J.; Bilek, Marcela M.M.; Jones, Robert T.; Pigram, Paul J.

doi:10.1002/app.33355

Cited by 22 publications

(14 citation statements)

References 62 publications

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“…Following bioconjugation of DOX to the PPP‐NH 2 ‐ g‐ PEG polymer, there appears an additional peak at 285.8 eV corresponding to the C in the C=N bonds of hydrazone (Figure B) . Moreover, the peak at 288.1 eV proves the amide bond formation between the PPP‐NH 2 ‐ g‐ PEG and Cys groups . These results confirm the successful conjugation of the PPP‐NH 2 ‐ g‐ PEG polymer and DOX.…”

Section: Resultssupporting

confidence: 57%

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Guler

Akbulut

Barlas

et al. 2016

Macromolecular Bioscience

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Conventional cancer treatments such as chemotherapy, radiotherapy, or combination of these two result in side effects, which lower the quality of life of the patients. To overcome problems with these methods, altering the drug properties by conjugating them to carrier polymers has emerged. Such polymeric carriers also hold the potential to make tumor cells more sensitive to radiation therapy. Herein, poly(p-phenylene) (PPP) polymer with poly(ethylene glycol) (PEG) chains and primary amino groups (PPP-NH2 -g-PEG) is synthesized and conjugated with anticancer drug Doxorubicin (DOX). pH dependent drug release experiments are performed at pH 5.3 and pH 7.4, respectively. Cell viability studies on human cervix adenocarcinoma cells show that lower doses of DOX inhibit cell proliferation when conjugated with nontoxic doses of PPP-NH2 -g-PEG polymer. Additionally, PPP-NH2 -g-PEG/Cys/DOX bioconjugate significantly increases radiosensitive properties of DOX. It is possible to use lower doses of DOX when conjugated to PPP-NH2 -g-PEG in combination with radiotherapy.

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Section: Resultssupporting

confidence: 57%

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Guler

Akbulut

Barlas

et al. 2016

Macromolecular Bioscience

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“…In preparations for enzyme membranes, it is difficult to get bulk active groups on the membrane surface. The traditional plasma surface physics modification [11,12] is complex and fussy and it has only a low effect on increasing immobilization of enzymes. The chemical modification of electrospun fibers has been shown to be an effective immobilization method compared with the physics modification.…”

Section: Introductionmentioning

confidence: 99%

Horseradish Peroxidase‐Carrying Electrospun Nonwoven Fabrics for the Treatment of o‐Methoxyphenol

Pan

Ding

et al. 2015

Journal of Nanomaterials

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The carboxyl-functionalized polystyrene (poly(styrene-co-methacrylic acid), PSMAA) nanofibers with average diameters of 250 ± 20 nm was prepared by electrospinning. PSMAA nanofibrous membrane were employed for immobilization of horseradish peroxidase (HRP) enzyme on the fibrous surface by a chemical method. The parameters about immobilizing HRP on the PSMAA nanofibers were studied and the influence on the activity of the HRP is discussed. This study showed that soap-free emulsion method is an ideal technology to modify the polystyrene surface and ultimately achieve enzyme immobilization on electrospun PSMAA nanofibers surfaces. Compared with free HRP, the acid-base stability, thermal stability, and storage stability of HRP were increased after the immobilization. The immobilized HRP maintained about 60% of its initial activity during a 20-day storage period. However, the free HRP maintained only 40% of its initial activity. The removal percentages of o-methoxyphenol (OMP) reached 80.2% after 120 min for immobilized HRP. These results suggest that the proposed scheme for immobilization of HRP has potential in industrial applications for the treatment of phenolic wastewater.

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“…The peak fitted components for GO and PA6 correspond with those in the literature. 27,28 The peak attributed to carboxyl (COOH), acyl chloride (RCOCl) 29 and amide (CONH 2 ) 30 arises at 288.8 eV (7.2%) for GO and is replaced by a peak at 289.2 eV (4.7%) for acyl-GO then a further displacement to 288.8 eV (5.3%) for amide-GO. This suggests successful chemical functionalization with a GO edge group conversion from carboxyl to acyl chloride and finally to amide moieties.…”

Section: Resultsmentioning

confidence: 99%

Preparation and properties of polyamide 6 nanocomposites covalently linked with amide functional graphene oxide

O’Neill

Bishop

Dalton

et al. 2016

Journal of Thermoplastic Composite Materials

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Chemically engineered polyamide 6 (PA6)/graphene oxide (GO) nanocomposites were produced via the functionalization of GO with an amide (CONH2) functional group, in order to produce amide-GO with improved interfacial bonding and dispersion in the host polymer matrix. In situ polymerization of ε-caprolactam was carried out in the presence of amide-GO to create PA6/amide-GO nanocomposites. The nanomaterial (pre- and post-polymerization) and the composites were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and tensile testing. The single-layer nature of GO was attested by TEM. FTIR, XPS, XRD and thermal analysis techniques confirmed the successful amide modification of GO. The expected attachment of PA6 to the surface of GO is demonstrated, along with the reduction of GO during polymerization. Some reduction of GO during the chemical functionalization process was also observed. The thermal stability of the nanocomposites was confirmed, while promotion of α-phase crystallite formation and a molecular weight change of attached PA6 are observed. A linear improvement in stiffness and yield strength was observed as functionalized GO content increased from 0.1 wt% to 0.75 wt%. A levelling off of mechanical properties ensued once the GO content reached 1 wt%, and a decrease was seen at 2 wt%.

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Surface attachment of horseradish peroxidase to nylon modified by plasma‐immersion ion implantation

Cited by 22 publications

References 62 publications

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Poly(p‐phenylene) with Poly(ethylene glycol) Chains and Amino Groups as a Functional Platform for Controlled Drug Release and Radiotherapy

Horseradish Peroxidase‐Carrying Electrospun Nonwoven Fabrics for the Treatment of o‐Methoxyphenol

Preparation and properties of polyamide 6 nanocomposites covalently linked with amide functional graphene oxide

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