Surface attached ultrathin polymer monolayers for control of cell adhesion

Dahm, Manfred; Chang, Bung‐Jun; Prucker, Oswald; Pierkes, Melanie; Alt, Tatjana; Mayer, Eckhard; Rühe, Jürgen; Oelert, H.

doi:10.1016/s0003-4975(01)02513-9

Cited by 19 publications

(14 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surface energy, which is intimately related to the contact angle, is one of the key factors governing cell/biomaterial interaction. It has been reported nevertheless that there is no obvious relationship between cell adhesion and surface energy . On the other hand, studies have revealed that better adhesion and cell responses are obtained in biomaterial surfaces with moderate hydrophilicity .…”

Section: Resultsmentioning

confidence: 99%

Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes

Ribeiro

Sencadas

Areias

et al. 2014

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses.

show abstract

Section: Resultsmentioning

confidence: 99%

Surface roughness dependent osteoblast and fibroblast response on poly(l-lactide) films and electrospun membranes

Ribeiro

Sencadas

Areias

et al. 2014

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…For example, Adden et al [ 32 ] reported a significantly restricted growth of osteogenic precursor cells on PMTA films. By contrast, early studies presented PMTA as highly adhesive surface for the human endothelial cell line, Hep G2 (human liver carcinoma) as well as rat and sheep fibrocytes [ 31 , 37 – 38 ]. Another study showed an increase in both protein adsorption and adhesion of MC3T3-E1 cells derived from newborn mouse calvaria with higher concentration of PMTA in the polyethylene glycol diacrylate (PEGDA) hydrogels [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Polymer Coatings of Cochlear Implant Electrode Surface – An Option for Improving Electrode-Nerve-Interface by Blocking Fibroblast Overgrowth

et al. 2016

View full text Add to dashboard Cite

Overgrowth of connective tissue and scar formation induced by the electrode array insertion increase the impedance and, thus, diminish the interactions between neural probes as like cochlear implants (CI) and the target tissue. Therefore, it is of great clinical interest to modify the carrier material of the electrodes to improve the electrode nerve interface for selective cell adhesion. On one side connective tissue growth needs to be reduced to avoid electrode array encapsulation, on the other side the carrier material should not compromise the interaction with neuronal cells. The present in vitro-study qualitatively and quantitatively characterises the interaction of fibroblasts, glial cells and spiral ganglion neurons (SGN) with ultrathin poly(N,N-dimethylacrylamide) (PDMAA), poly(2-ethyloxazoline) (PEtOx) and poly([2-methacryloyloxy)ethyl]trimethylammoniumchlorid) (PMTA) films immobilised onto glass surfaces using a photoreactive anchor layer. The layer thickness and hydrophilicity of the polymer films were characterised by ellipsometric and water contact angle measurement. Moreover the topography of the surfaces was investigated using atomic force microscopy (AFM). The neuronal and non-neuronal cells were dissociated from spiral ganglions of postnatal rats and cultivated for 48 h on top of the polymer coatings. Immunocytochemical staining of neuronal and intermediary filaments revealed that glial cells predominantly attached on PMTA films, but not on PDMAA and PEtOx monolayers. Hereby, strong survival rates and neurite outgrowth were only found on PMTA, whereas PDMAA and PEtOx coatings significantly reduced the SG neuron survival and neuritogenesis. As also shown by scanning electron microscopy (SEM) SGN strongly survived and retained their differentiated phenotype only on PMTA. In conclusion, survival and neuritogenesis of SGN may be associated with the extent of the glial cell growth. Since PMTA was the only of the polar polymers used in this study bearing a cationic charge, it can be assumed that this charge favours adhesion of both glial cells and SG neurons glial cells and SGN.

show abstract

“…After careful removal of all non-attached polymer through an extensive extraction procedure (Soxhlet extraction overnight) the substrates were brought into contact with cells suspended in appropriate culture media. It was observed that some polymer coatings such as polyethyloxazoline exhibited very good cell adhesion while other polymers, most noticeably polyacrylamide proved to be very non-attractive for the cells [12,13] (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%