Surface antigens of male worms and microfilariae of Onchocerca gibsoni

Gárate, Teresa; Cabrera, Z.; Copeman, D. B.; Harnett, William; McLaren, Diane J.; Patterson, M. K.; Parkhouse, R. M. E.

doi:10.1016/0020-7519(91)90118-q

Cited by 6 publications

(2 citation statements)

References 22 publications

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“…Polypeptide profiles obtained from iodination of the parasites surface may be dependent on the technique employed. Maizels et al, (1986), Marshall & Howells (1985) Maizels et al, (1989) and Garate et al, (1991) from those generated by the Bolton/Hunter surface labelling technique. Both Iodogen and Bolton/Hunter procedures were, therefore, utilized during these studies on the third stage infective larvae of both strains.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the surface polypeptides of Strongyloides ratti: a comparison of homogonic and heterogonic strains

Power

Harnett²,

Jenkins

1994

J. Helminthol.

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Surface iodination, extraction and SDS-PAGE analysis techniques were employed to characterize and compare the surface polypeptides of two strains of Strongyloides ratti. Third stage infective larvae and parasitic adults of homogonic and heterogonic strains were studied using a variety of surface labelling procedures and detergents for the extraction of labelled molecules. Profiles obtained from SDS-PAGE analysis demonstrated that homogonic and heterogonic strains of S. ratti have identical surface antigens.

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Section: Discussionmentioning

confidence: 99%

Characterization of the surface polypeptides of Strongyloides ratti: a comparison of homogonic and heterogonic strains

Power

Harnett²,

Jenkins

1994

J. Helminthol.

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“…The use of cattle models for treatment research and the development of markers that differentiate O. volvulus from the cattle parasites transmitted by the same vector species of blackflies (Diptera: Simuliidae), has been of major interest (Muller, 1979;Copeman, 1993). The presumed close taxonomic relationship between O. volvulus, O. gibsoni and O. ochengi (Bain, 1981) has stimulated the use of the two cattle parasites as model species for research into human onchocerciasis treatment and detection (Vankan et al, 1988;Garate et al, 1991;Trees et al, 2000). However, the taxonomic relationships between O. volvulus, O. ochengi and O. gibsoni are controversial and remain to be clarified.…”

Section: Introductionmentioning

confidence: 99%

Molecular systematics of five Onchocerca species (Nematoda: Filarioidea) including the human parasite, O. volvulus, suggest sympatric speciation

Morales-Hojas,

Cheke,

Post

2006

J. Helminthol.

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The genus Onchocerca (Nematoda: Filarioidea) consists of parasites of ungulate mammals with the exception of O. volvulus, which is a human parasite. The relationship between O. volvulus, O. ochengi and O. gibsoni remains unresolved. Based on morphology of the microfilariae and infective larvae, vector transmission and geographical distribution, O. ochengi and O. volvulus have been placed as sister species. Nevertheless, the cuticle morphology and chromosomal data (O. volvulus and O. gibsoni have n=4 while O. ochengi is n=5) suggest that O. gibsoni could be more closely related to O. volvulus than O. ochengi. Sequences from the 12S rRNA, 16S rRNA and ND5 mitochondrial genes have been used to reconstruct the phylogeny of five Onchocerca species including O. volvulus. Analyses with maximum likelihood and maximum parsimony showed that O. ochengi is the sister species of O. volvulus, in accordance with the classification based on morphology and geographical location. The separate specific status of the species O. gutturosa and O. lienalis was supported, although their phylogenetic relationship was not well resolved. The analyses indicated that the basal species was O. gibsoni, a South-East Asian and Australasian species, but this result was not statistically significant. The possible involvement of sympatric speciation in the evolution of this group of parasites is discussed.

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Biosynthetic radiolabelling of excretions-secretions of adult male Onchocerca gibsoni

Harnett¹,

Patterson

Copeman

et al. 1994

International Journal for Parasitology

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Surface antigens of male worms and microfilariae of Onchocerca gibsoni

Cited by 6 publications

References 22 publications

Characterization of the surface polypeptides of Strongyloides ratti: a comparison of homogonic and heterogonic strains

Characterization of the surface polypeptides of Strongyloides ratti: a comparison of homogonic and heterogonic strains

Molecular systematics of five Onchocerca species (Nematoda: Filarioidea) including the human parasite, O. volvulus, suggest sympatric speciation

Biosynthetic radiolabelling of excretions-secretions of adult male Onchocerca gibsoni

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