Surface antigens of cell subpopulations in prenatal rat brain are expressed in a characteristic non-random pattern on their ethylnitrosourea-induced malignant counterparts

Kindler‐Röhrborn, Andrea; Blass-Kampmann, Sabine; Lennartz, Klaus; Liepelt, Ursula; Minwegen, Romano; Rajewsky, Manfred F.

doi:10.1046/j.1432-0436.1994.5730215.x

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…The expression profile of the three cell surface antigens on PRD 18 rat brain cells has been reported by Kindler-Röhrborn et al (1994). At this developmental stage, 8.1% 6 2.3% (SD) of all brain cells were gp130 RB13-6 positive (Fig.…”

Section: Double-immunofluorescence Fca Of Gp130 Rb13-6 -Positive Braimentioning

confidence: 66%

“…This glycoprotein specified by monoclonal antibody (mAb) RB13-6 (Kindler-Röhrborn et al, 1985) is related to the plasma cell membrane protein PC-1 (van Driel and Goding, 1987), a nucleotide pyrophosphatase/ alkaline phosphodiesterase and ectoprotein kinase, and to the tumor cell motility factor autotaxin (Murata et al, 1994). The gp130 protein had been shown to be transiently expressed by a small subpopulation (,8%) of BDIX-rat fetal brain cells (FBC) on PRD 18 and also by the cells of most malignant mixed gliomas induced in the same inbred rat strain on PRD 18 by a single transplacental pulse of the DNA-reactive carcinogen N-ethyl-Nnitrosourea (EtNU;Kindler-Röhrborn et al, 1994). In long-term cultures of FBC isolated 24 hr after in vivo exposure to EtNU, gp130 -positive precursor cells exhibited a sustained proliferative advantage and ultimately underwent tumorigenic conversion (S. BlassKampmann, T. Bilzer, and M.F.…”

Section: Introductionmentioning

confidence: 99%

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In vitro differentiation of neural progenitor cells from prenatal rat brain: Common cell surface glycoprotein on three glial cell subsets

Blass-Kampmann¹,

Kindler‐Röhrborn²,

Deissler³

et al. 1997

J. Neurosci. Res.

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Section: Double-immunofluorescence Fca Of Gp130 Rb13-6 -Positive Braimentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

In vitro differentiation of neural progenitor cells from prenatal rat brain: Common cell surface glycoprotein on three glial cell subsets

Blass-Kampmann¹,

Kindler‐Röhrborn²,

Deissler³

et al. 1997

J. Neurosci. Res.

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“…This same monoclonal antibody was also found to react with several primary brain tumors and brain tumor cell lines that had been induced by N-ethyl-N-nitrosourea (31). Gp130 , related to both ATX and PC-1, has recently been shown to possess 5Ј-nucleotide PDE I activity (8).…”

mentioning

confidence: 88%

Stimulation of Tumor Cell Motility Linked to Phosphodiesterase Catalytic Site of Autotaxin

Lee

Clair

Mulvaney

et al. 1996

Journal of Biological Chemistry

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“…Based on the largely circumstantial evidence now available, it appears that the NPPs might be important in di erentiation and development and might have a complex relationship to carcinogenesis. Overexpression of B-10 has been noted on the cell surface of ethylnitrosourea-induced rat brain tumors (Kindler-Rohrborn et al, 1994). ATX expression is elevated in human neuroblastoma and stimulates cell motility in a neuroblastoma cell line (Kawagoe et al, 1997).…”

Section: Lung Metastasis Incidencementioning

confidence: 99%

Autotaxin (ATX), a potent tumor motogen, augments invasive and metastatic potential of ras-transformed cells

et al. 2000

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Autotaxin (ATX), an exo-nucleotide pyrophosphatase and phosphodiesterase, was originally isolated as a potent stimulator of tumor cell motility. In order to study whether ATX expression a ects motility-dependent processes such as invasion and metastasis, we stably transfected full-length ATX cDNA into two nonexpressing cell lines, parental and ras-transformed NIH3T3 (clone7) cells. The e ect of ATX secretion on in vitro cell motility was variable. The ras-transformed, ATX-secreting subclones had enhanced motility to ATX as chemoattractant, but there was little di erence in the motility responses of NIH3T3 cells transfected with atx, an inactive mutant gene, or empty vector. In Matrigel TM invasion assays, all subclones, which secreted enzymatically active ATX, demonstrated greater spontaneous and ATX-stimulated invasion than appropriate controls. This di erence in invasiveness was not caused by di erences in gelatinase production, which was constant within each group of transfectants. In vivo studies with athymic nude mice demonstrated that injection of atx-transfected NIH3T3 cells resulted in a weak tumorigenic capacity with few experimental metastases. Combination of ATX expression with ras transformation produced cells with greatly ampli®ed tumorigenesis and metastatic potential compared to ras-transformed controls. Thus, ATX appears to augment cellular characteristics necessary for tumor aggressiveness.

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Surface antigens of cell subpopulations in prenatal rat brain are expressed in a characteristic non-random pattern on their ethylnitrosourea-induced malignant counterparts

Cited by 7 publications

References 22 publications

In vitro differentiation of neural progenitor cells from prenatal rat brain: Common cell surface glycoprotein on three glial cell subsets

In vitro differentiation of neural progenitor cells from prenatal rat brain: Common cell surface glycoprotein on three glial cell subsets

Stimulation of Tumor Cell Motility Linked to Phosphodiesterase Catalytic Site of Autotaxin

Autotaxin (ATX), a potent tumor motogen, augments invasive and metastatic potential of ras-transformed cells

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