Surface alteration of Saccharomyces cerevisiae induced by thymol and eugenol

Abstract

Aims: This study aims to bring some information about the mechanism of the fungicidal action of thymol and eugenol; phenolic major components of thyme and clove essential oils respectively. Saccharomyces cerevisiae was used as yeast model. Methods and Results: Treatment of yeast cells with these components led to their lysis as shown by the release of substances absorbing at 260 nm. In addition, scanning electron microscope observations revealed that the surface of the treated cells was significantly damaged. Conclusions: Antifungal activity of thymol and eugenol involve alteration of both membrane and cell wall of the yeast. Significance and Impact of the Study: This work is a preliminary contribution aiming to develop a new generation of efficient and natural antifungal agents.

Eugenol, an essential oil of clove, causes disruption to the cell wall of Candida albicans (ATCC 14053)

“…concentrationdependent and time-course lysis. The procedures used to assess both variables were carried out at an absorbance wavelength of 260 nm, as suggested by Bennis et al (2004).…”

Efficacy of Origanum essential oils for inhibition of potentially pathogenic fungi

“…The essential oils were tested in the range of 320 -0.03 µL/ mL. Solutions of essential oils were prepared in Sabouraud Broth (SB) using bacteriological agar (0.15 g/100mL) as the stabilizing agent according to Bennis et al (2004). For antifungal assays, the fungi were cultivated on SA slants for 24-48 h at 37 ºC and for 10-14 days at 28 ºC, for yeasts and moulds, respectively.…”

Keefektifan Pelapisan Benih terhadap Peningkatan Mutu Benih Padi Selama Penyimpanan

“…Kerusakan sel diawali dengan rusaknya membran sel yang berlanjut dengan keluarnya material isi sel dan akhirnya sel mengalami kematian (Mangoni et al 2004, Rasooli et al 2006. Kim et al (1995) dan Bennis et al (2004) dan menurunkan daya berkecambah benih lebih cepat dibanding pestisida kimia yang berbentuk serbuk padat. Kadar air pada semua perlakuan coating sudah berbeda nyata dengan kontrol sejak awal penyimpanan (Gambar 8).…”