Surface activation of thin polyvinyl alcohol films by atmospheric pressure plasma jet: Influence of electron temperature

Lamichhane, Prajwal; Acharya, Tirtha R.; Park, JaeWoo; Amsalu, Kirubel A.; Park, Byoungchoo; Choi, Eun Ha

doi:10.1002/ppap.202300102

Cited by 20 publications

(3 citation statements)

References 40 publications

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“…Soft jet plasmas are created by applying electrical energy to ambient air, creating a high-energy environment that facilitates unique reactions forming various RONS 41,42 . The formation of nitrogen oxides (NO x ), including NO (nitric oxide), NO 2 (nitrogen dioxide), and N 2 O (nitrous oxide), in a soft jet plasma involves complex chemical reactions 13 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of direct air gas soft jet plasma for oral bacterial biofilm eradication and treatment

Puca,

Marinacci,

Pinti

et al. 2024

Preprint

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The aim of this study was to evaluate the antimicrobial efficacy of an air gas soft jet CAP for its potential use in removing oral biofilms, given that plasma-based technologies have emerged as promising methods in periodontology. Two types of biofilms were developed, one by Streptococcus mutans UA 159 bacterial strain and the other by a complex mixture of saliva microorganisms isolated from a patient with periodontitis. This latter biofilm was characterized via Next Generation Sequencing to determine the main bacterial phyla. The CAP source was applied at a distance of 6mm for different time points. A statistically significant reduction of both CFU count and XTT was detected after 60s of CAP treatment, while the treatment for 120s resulted in both biofilms eradication. CLSM analysis supported CAP effectiveness in killing the microorganisms inside the biofilm and in reducing the thickness of the biofilm matrix. Cytotoxicity tests demonstrated the possible use of CAP without important side effects towards human gingival fibroblasts cell line. The current study showed that CAP treatment was able to eradicate preformed biofilms developed by both S. mutans and the complex mixture of saliva microorganisms, representing a potential innovative strategy to counteract oral pathogens responsible for periodontal diseases.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of direct air gas soft jet plasma for oral bacterial biofilm eradication and treatment

Puca,

Marinacci,

Pinti

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Soft jet plasmas are created by applying electrical energy to ambient air, creating a high-energy environment that facilitates unique reactions forming various ROS and RNS 43 , 44 . The formation of nitrogen oxides (NO x ), including NO (nitric oxide), NO 2 (nitrogen dioxide), and N 2 O (nitrous oxide), in a soft jet plasma involves complex chemical reactions 13 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms

Puca,

Marinacci,

Pinti

et al. 2024

Sci Rep

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The aim of this study was to evaluate the antimicrobial efficacy of an air gas soft jet CAP for its potential use in removing oral biofilms, given that plasma-based technologies have emerged as promising methods in periodontology. Two types of biofilms were developed, one by Streptococcus mutans UA 159 bacterial strain and the other by a complex mixture of saliva microorganisms isolated from a patient with periodontitis. This latter biofilm was characterized via Next Generation Sequencing to determine the main bacterial phyla. The CAP source was applied at a distance of 6 mm for different time points. A statistically significant reduction of both CFU count and XTT was already detected after 60 s of CAP treatment. CLSM analysis supported CAP effectiveness in killing the microorganisms inside the biofilm and in reducing the thickness of the biofilm matrix. Cytotoxicity tests demonstrated the possible use of CAP without important side effects towards human gingival fibroblasts cell line. The current study showed that CAP treatment was able to significantly reduce preformed biofilms developed by both S. mutans and microorganisms isolated by a saliva sample. Further studies should be conducted on biofilms developed by additional saliva donors to support the potential of this innovative strategy to counteract oral pathogens responsible for periodontal diseases.

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“…The electron temperature (kT e ), electron density (N e ), and the densities of nitrogen meta-stable states (N A , N B , and N C ) were measured using modi ed Nitrogen collisional radiative model, as extensively elucidated in our previous study [30]. Additionally, the rotational temperature (Tg) and vibrational temperature (Tv) of the plasma were calculated using a Boltzmann plot, speci cally employing the nitrogen second positive system, as previously described in our earlier work [31]. Gas-phase Fourier transform infrared spectroscopy (FTIR) was used along with OPUS GA software and the automated FTIR gas analyser MATRIX-MG5 to analyse the plasma in realtime.…”

Section: Plasma System Diagnostics and Rons Measurement Techniquementioning

confidence: 99%

Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines

Giacomo,

Balaha,

Pinti

et al. 2023

Preprint

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Objective: Cold atmospheric plasma (CAP) is a novel therapeutic approach for cancer treatment. It can be used to treat liquids - plasma-activated media (PAM) - which are then transferred to the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM and assessing their in vitro selectivity against head and neck cancer cell lines (HNC). Materials and methods: PAM were obtained by exposing 2 and 5 mL of medium to CAP for 5, 10 and 20 minutes at a 6 mm working distance. Anions kinetics was evaluated by ion chromatography. In addition, inhibition of cell proliferation by MTS assay, apoptosis occurrence and cell cycle modifications by flow cytometry were assessed on primary human gingival fibroblasts (hGF) and the HNC cell lines HSC2, HSC4 and A253. Results: All the 2 mL conditions showed a significant reduction in cell proliferation whereas for the 5 mL the effect was milder, but the time-dependence was more evident. In addition, hGF were unaffected by the 5 mL PAM, indicating a selectivity for cancer cells. Conclusions: The media chemical composition modified by CAP exposure influenced cell proliferation by modulating cell cycle and inducing apoptosis in cancer cells, without affecting normal cells. Clinical Relevance: The present investigation represents a starting point to favour the clinical translation of CAP as a precision medicine tool by proposing an innovative method, namely ion chromatography, to standardize the quantification of plasma-derived RONS and proving its selectivity in inactivating tumor cells over non-malignant cells. These strategies could be applied to identify the optimal parameter configuration to achieve the desired treatment/therapeutic outcome and to aid the definition of clinical protocols.

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Surface activation of thin polyvinyl alcohol films by atmospheric pressure plasma jet: Influence of electron temperature

Cited by 20 publications

References 40 publications

Antimicrobial efficacy of direct air gas soft jet plasma for oral bacterial biofilm eradication and treatment

Antimicrobial efficacy of direct air gas soft jet plasma for oral bacterial biofilm eradication and treatment

Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms

Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines

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