Surface Activation of Poly(Methyl methacrylate) by Plasma Treatment: Stable Antibody Immobilization for Microfluidic Enzyme-Linked Immunosorbent Assay

Darain, Farzana; Wahab, Md. Abdul; Tjin, Swee Chuan

doi:10.1080/00032719.2012.698673

Cited by 20 publications

(15 citation statements)

References 14 publications

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“…Finally, the methyl carboxylate groups on the nanosphere surface are readily available for surface grafting and surface functionalization. Toward further customization of the CPB@PMMA nanospheres, they were subjected to oxygen plasma treatment (see the Supporting Information for conditions), resulting in the exchange of C(O)OCH 3 groups for relatively more polar and more reactive C(O)OH groups . In the FTIR spectra of the CPB@PMMA nanospheres after plasma treatment (black line in Figure S12 in the Supporting Information), the distinct carboxylic stretching and bending vibrations of the PMMA stayed intact at 1722.8 and 1143.3 cm −1 for CO and CO, respectively.…”

Section: Resultsmentioning

confidence: 99%

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr₃@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Wang

Váradi

Trinchi

et al. 2018

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106

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Despite their impressive optical properties, lead halide perovskite quantum dots (PQDs) have not realized their potential, especially in bioimaging applications, as they suffer from poor moisture and thermal stability, solvent incompatibility, and significant toxicity. Here, a spray‐assisted coil–globule transition method for encapsulating CsPbBr3 (CPB) PQDs into poly(methyl methacrylate) (PMMA) polymer nanospheres is reported. Polyvinylpyrrolidone‐capped CPB PQDs are synthesized via the ligand assisted reprecipitation method in dichloromethane. After dissolving PMMA, the above precursor solution is sprayed into petroleum ether under high pressure N2. High‐pressure nebulization restricts the interactions between PMMA polymer chains, resulting in the formation of ≈112 nm nanoscale composite spheres after a coil–globule transition. The CPB@PMMA nanospheres not only possess 73% quantum yields but retain 81% of fluorescence intensity after the exposure to water for over 80 days. Due to their confined size and biocompatible encapsulation, they are readily available for cellular uptake and exhibit no toxicity on live HeLa cells. Furthermore, the PMMA surface allows for functional surface modification, carrying the possibility of targeting specific biological species and processes.

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Section: Resultsmentioning

confidence: 99%

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr₃@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Wang

Váradi

Trinchi

et al. 2018

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106

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“…Microfluidic chip technology allows conventional bench systems to ''shrink'' to a few square centimeters with advantages of speed, performance, integration, portability, reduced sample and solvent consumption, automation, and lower cost (Darain, Wahab, and Tjin 2012). Microfluidic chips are used for on-site analysis of heavy metals (Zhao et al 2009;Horstkotte, Duarte, and Cerdà 2013), organic pollutants (Lafleur et al 2010;B.…”

Section: Introductionmentioning

confidence: 99%

Portable Microfluidic Chip Based Surface-Enhanced Raman Spectroscopy Sensor for Crystal Violet

Liu

Yang

et al. 2014

Analytical Letters

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This paper describes the development of a portable microfluidic chip based on a surface-enhanced Raman spectroscopy (SERS) sensor for crystal violet analysis. A Y-shape microfluidic chip with a staggered herringbone structure was designed to efficiently mix the analyte and SERS active silver colloid. The subsequent detection of the analyte was performed on the microfluidic chip by a portable Raman system. Compared with other methods, this sensor is easy to operate and is expected to have applications for rapid and sensitive on-site analysis. A good linear correlation over the concentration range of 10 to 750 nM of crystal violet with a correlation coefficient of 0.992 was obtained. The recovery was between 98.6% and 102.9% for crystal violet in river water with relative standard deviations between 2.43% and 4.26%.

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“…Previously it has been reported that adsorption behavior of biomolecules depends on the type of protein molecules and nature of solid supports [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][13][14][15][16][17][18][19][20][48][49][50][51][52]. In our case dimensions of BSA are 4.7 nm × 4.7 nm × 11 nm, whereas C 60 -FMC adsorbent shows characteristics of bimodal pore size of 4.95 nm and 10-15 nm.…”

Section: The Adsorption Performance Of Fullerene Mesoporous Carbon Mamentioning

confidence: 93%

Nano-Confined Synthesis of Fullerene Mesoporous Carbon (C60-FMC) with Bimodal Pores: XRD, TEM, Structural Properties, NMR, and Protein Immobilization

Wahab¹,

Darain²,

Karim³

et al. 2015

Int J Nanomater Nanotechnol Nanomed

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Nanoconfined synthesized crystalline fullerene mesoporous carbon (C 60 -FMC) with bimodal pore architectures of 4.95 nm and 10-15 nm pore sizes characterized by XRD, TEM, nitrogen adsorption/ desorption isotherm and solid-state NMR, and the material was used for protein immobilization. The solid-state 13 C NMR spectrum of C 60 -FMC along with XRD, BET and TEM confirms the formation of fullerene mesoporous carbon structure C 60 -FMC. The immobilization of albumin (from bovine serum, BSA) protein biomolecule in a buffer solution at pH 4.7 was used to determine the adsorption properties of the C 60 -FMC material and its structural changes investigated by FT-IR. We demonstrated that the C 60 -FMC with high surface area and pore volumes have excellent adsorption capacity towards BSA protein molecule. Protein adsorption experiments clearly showed that the C 60 -FMC with bimodal pore architectures (4.95 nm and 10-15 nm) are suitable material to be used for protein adsorption.and biomolecules or drugs adsorption on mesoporous carbons have been reported in the literatures [13][14][15][16][17][18][19][20]. It is also observed that mesoporous carbon materials exhibited better performance for these applications than that of counterpart silica material. Then, instead of using a conventional carbon source as mentioned above [1][2][3][4][12][13][14][15][16][17][18][19][20] it will be necessary to find a new carbon source to create a new mesoporous carbon solid framework that will improve the adsorption of biomolecules. As such, Fullerenes (C 60 ) as carbon source consists of nanoscopic building block with surface tailorable that can give unique functionalities to the mesoporous carbon framework [21][22][23]. Previously, fullerene C 60 has been partly used along with porous silica for extending their surface functionalities [24][25][26][27][28][29][30][31][32] but C 60 has not been reported to be used as sole carbon source for preparing fullerene mesoporous carbon that can be useful for protein immobilization.With this concept in mind, this paper, successfully demonstrate the use of fullerene C 60 as carbon source to prepare a new class of fullerene mesoporous carbon (C 60 -FMC) with bimodal pore architectures via pore filling method and its properties for protein immobilization. Experimental Section MaterialsTetraethylorthosilicate (TEOS), Pluronic surfactant P123 (EO20PPO70EO20), Fullerene C 60 , Trimethylbenzene (TMB) and Albumin from Bovine serum (BSA) from Sigma-Aldrich were used as received without further purification.

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Surface Activation of Poly(Methyl methacrylate) by Plasma Treatment: Stable Antibody Immobilization for Microfluidic Enzyme-Linked Immunosorbent Assay

Cited by 20 publications

References 14 publications

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr₃@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr₃@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Portable Microfluidic Chip Based Surface-Enhanced Raman Spectroscopy Sensor for Crystal Violet

Nano-Confined Synthesis of Fullerene Mesoporous Carbon (C60-FMC) with Bimodal Pores: XRD, TEM, Structural Properties, NMR, and Protein Immobilization

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Surface Activation of Poly(Methyl methacrylate) by Plasma Treatment: Stable Antibody Immobilization for Microfluidic Enzyme-Linked Immunosorbent Assay

Cited by 20 publications

References 14 publications

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr3@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr3@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Portable Microfluidic Chip Based Surface-Enhanced Raman Spectroscopy Sensor for Crystal Violet

Nano-Confined Synthesis of Fullerene Mesoporous Carbon (C60-FMC) with Bimodal Pores: XRD, TEM, Structural Properties, NMR, and Protein Immobilization

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Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr₃@PMMA Perovskite Nanospheres with Application in Live Cell Imaging

Spray‐Assisted Coil–Globule Transition for Scalable Preparation of Water‐Resistant CsPbBr₃@PMMA Perovskite Nanospheres with Application in Live Cell Imaging