Surface‐activated chemical ionization and high‐flow gradient chromatography to reduce matrix effect

Cristoni, Simone; Bernardi, L.; Gerthoux, Piermario; Mocarelli, Paolo; Brambilla, Maura; Gonella, Elisabetta; Guidugli, Federico; Cantù, Marco

doi:10.1002/rcm.2599

Cited by 12 publications

(17 citation statements)

References 29 publications

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“…In particular, recently an interesting application on the elimination of matrix effect in the analysis of Tacrolimus, an important immunosuppressive agent for renal transplantation, in diluted plasma samples after protein precipitation has been published (Cristoni et al, 2006b). SACI was used in conjunction with high eluent flow gradient chromatography (eluent flow 1,300 mL/min) showing that under these conditions, the matrix effect due to contaminants extracted from plasma was avoided.…”

Section: Advantages Of the Saci Technologymentioning

confidence: 99%

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Development and applications of surface‐activated chemical ionization

Cristoni

Rubini²,

Bernardi

2007

Mass Spectrometry Reviews

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This review regards the recently developed ionization source named surface-activated chemical ionization (SACI) that employs an interaction with a surface placed at low voltage for the activation of the ionization of sample molecules to increase the sensitivity in the analysis of various compounds of biological and clinical interest. These results are due to the strong chemical noise decrease and the increase of ionization efficiency. This ionization source has been employed for the analysis of various compounds of different molecular mass and polarity (addicted and pharmaceutical drugs, amino acids, steroids, peptides, and proteins). The SACI development theoretical mechanism, benefits, disadvantages, applications, and future developments are reported and discussed.

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Section: Advantages Of the Saci Technologymentioning

confidence: 99%

“…drugs (Cristoni et al, 2004a(Cristoni et al, , 2005c(Cristoni et al, , 2006b 2. amino acids (Cristoni et al, 2005b) 3. steroids (Cristoni et al, 2004b).…”

Section: A Analysis Of Addict Drugs and Small Moleculesunclassified

Development and applications of surface‐activated chemical ionization

Cristoni

Rubini²,

Bernardi

2007

Mass Spectrometry Reviews

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“…[2] Moreover, the increased benefits of SACI in terms of quantitation accuracy, precision and matrix effect reduction have been widely shown. [2,3] The two commonly used atmospheric pressure ionization sources, electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), can be influenced by the matrix effect. [4] SACI is, however, less affected by matrix signal suppression effects.…”

Section: Introductionmentioning

confidence: 99%

“…[4] SACI is, however, less affected by matrix signal suppression effects. [3,5] For example, analyzing undiluted red wine and grape matrix to quantitate ocratoxin does not affect the quantitative performance of SACI. [5] Moreover, the matrix effect is strongly reduced with SACI, as compared with ESI and APCI, even in the presence of salts and buffers.…”

Section: Introductionmentioning

confidence: 99%

“…[5] Moreover, the matrix effect is strongly reduced with SACI, as compared with ESI and APCI, even in the presence of salts and buffers. [3] In particular, SACI works under ion exchange chromatography (IEC) conditions because of the low signal suppression effect that occurs when working in low potential ionization conditions (50/400 V). [6] None of the other atmospheric pressure ionization sources can work under these conditions because of in-source discharge phenomena.…”

Section: Introductionmentioning

confidence: 99%

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Surface‐activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A

et al. 2009

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Surface-activated chemical ionization (SACI) has been widely used in recent years for the analysis of different compounds (e.g. peptides, street drugs, amino acids). The main benefits of this technology are its high sensitivity and its effectiveness under different chromatographic conditions [i.e. ion exchange chromatography and reversed-phase (RP) chromatography]. Here we used SACI in conjunction with quadrupole time-of-flight mass spectrometry to analyze enterotoxin A, which is produced by Staphylococcus aureus, in milk matrix using both RP and ion exchange chromatographies. SACI had increased sensitivity as compared with electrospray ionization. Moreover, the higher quantitation efficiency of this technique, mainly in terms of limit of detection (0.01 ng/ml), limit of quantitation (0.05 ng/ml), linearity range (0.05-50 ng/ml), matrix effect, accuracy (intraday and interday accuracy errors were 9.2% and 10.3%, respectively) and precision (intraday and interday precision errors were 5.3% and 12.8%, respectively), is shown and discussed.

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Current literature in mass spectrometry

2007

J. Mass Spectrom.

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Surface‐activated chemical ionization and high‐flow gradient chromatography to reduce matrix effect

Cited by 12 publications

References 29 publications

Development and applications of surface‐activated chemical ionization

Development and applications of surface‐activated chemical ionization

Surface‐activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A

Current literature in mass spectrometry

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