Surface acoustic wave (SAW) acoustophoresis: now and beyond

Lin, Szu-Yuan; Mao, Xiaole; Huang, Tony Jun

doi:10.1039/c2lc90076a

Cited by 195 publications

(144 citation statements)

References 35 publications

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“…[147][148][149] The acoustic technique has three merits: (a) the capability of manipulating most microparticles, regardless of optical, electrical, magnetic, or shape properties; (b) the ability to manipulate objects with a range of scales, from nanometer to millimeter; and (c) the capability of rapidly manipulating a single particle or groups of particles.…”

Section: E Acoustic Trapsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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Section: E Acoustic Trapsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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“…In addition to the surface acoustic wave along the surface of the piezoelectric substrate, but also has many advantages [1]: 1) SAW propagation on a piezoelectric substrate has a very low propagation velocity and a very short wavelength. And their respective transmission over the corresponding electromagnetic waves speed and wavelength is five orders of magnitude.…”

Section: Introductionmentioning

confidence: 99%

Surface Acoustic Wave Technology and Its Application

Li¹

2017

OALib

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This paper introduces briefly the structures, specifications and applications of surface acoustic wave (SAW) sensors, as well as the progresses in the related studies. The SAW sensors include Rayleigh wave, shear horizontal acoustic plate wave, Love wave and Lamb wave sensors. The application fields are classified according to the functions into physical sensors and chemical/biological sensors; the former can be performed more easily, but the latter homes special functions and so needs to be studied in more details based on the practical conditions. The SAW sensors have very high sensitivity and wide applications due to the velocities and phase of SAW are sensitive to the parameter changes of environments.

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“…2,3 The most commonly used enrichment techniques are fluorescent activated cell sorting (FACS) 4 and magnetic activated cell sorting (MACS), 5 which rely upon expressed surface markers to differentiate cells. Other strategies include hydrodynamic 6 or inertial 7,8 sorting, optical tweezers, 9 acoustophoresis, 10 and dielectrophoresis.…”

Section: Introductionmentioning

confidence: 99%

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Elvington

Salmanzadeh

Stremler

et al. 2013

JoVE

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Dielectrophoresis (DEP) is the phenomenon by which polarized particles in a non-uniform electric field undergo translational motion, and can be used to direct the motion of microparticles in a surface marker-independent manner. Traditionally, DEP devices include planar metallic electrodes patterned in the sample channel. This approach can be expensive and requires a specialized cleanroom environment. Recently, a contact-free approach called contactless dielectrophoresis (cDEP) has been developed. This method utilizes the classic principle of DEP while avoiding direct contact between electrodes and sample by patterning fluidic electrodes and a sample channel from a single polydimethylsiloxane (PDMS) substrate, and has application as a rapid microfluidic strategy designed to sort and enrich microparticles. Unique to this method is that the electric field is generated via fluidic electrode channels containing a highly conductive fluid, which are separated from the sample channel by a thin insulating barrier. Because metal electrodes do not directly contact the sample, electrolysis, electrode delamination, and sample contamination are avoided. Additionally, this enables an inexpensive and simple fabrication process. cDEP is thus well-suited for manipulating sensitive biological particles. The dielectrophoretic force acting upon the particles depends not only upon spatial gradients of the electric field generated by customizable design of the device geometry, but the intrinsic biophysical properties of the cell. As such, cDEP is a label-free technique that avoids depending upon surface-expressed molecular biomarkers that may be variably expressed within a population, while still allowing characterization, enrichment, and sorting of bioparticles.Here, we demonstrate the basics of fabrication and experimentation using cDEP. We explain the simple preparation of a cDEP chip using soft lithography techniques. We discuss the experimental procedure for characterizing crossover frequency of a particle or cell, the frequency at which the dielectrophoretic force is zero. Finally, we demonstrate the use of this technique for sorting a mixture of ovarian cancer cells and fluorescing microspheres (beads).

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Surface acoustic wave (SAW) acoustophoresis: now and beyond

Cited by 195 publications

References 35 publications

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Surface Acoustic Wave Technology and Its Application

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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