Surface acoustic wave microfluidics for repetitive and reversible temporary immobilization of <i>C. elegans</i>

Sridhar, Nakul; Fajrial, Apresio Kefin; Doser, Rachel L; Hoerndli, Frédéric; Ding, Xiaoyun

doi:10.1039/d2lc00737a

Cited by 5 publications

(4 citation statements)

References 56 publications

(75 reference statements)

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“…By adjusting the duration and power intensity of SAW actuation, an optimal exposure setting has been found that substantially mitigates neurodegenerations in both models. This research underscores the nuanced relationship between exercise parameters and neuroprotection in C. elegans, unveiling the acoustofluidic gym as an instrumental tool for further inquiries in this domain [141,142]. More clinically, Gai et al have contributed an innovative, automated methodology for the selection of high-quality sperm, targeting the resolution of male infertility [143,144].…”

Section: Acoustofluidic Biophysical Therapymentioning

confidence: 86%

Acoustofluidic Actuation of Living Cells

Wu,

Gai,

Zhao

et al. 2024

Micromachines

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Acoutofluidics is an increasingly developing and maturing technical discipline. With the advantages of being label-free, non-contact, bio-friendly, high-resolution, and remote-controllable, it is very suitable for the operation of living cells. After decades of fundamental laboratory research, its technical principles have become increasingly clear, and its manufacturing technology has gradually become popularized. Presently, various imaginative applications continue to emerge and are constantly being improved. Here, we introduce the development of acoustofluidic actuation technology from the perspective of related manipulation applications on living cells. Among them, we focus on the main development directions such as acoustofluidic sorting, acoustofluidic tissue engineering, acoustofluidic microscopy, and acoustofluidic biophysical therapy. This review aims to provide a concise summary of the current state of research and bridge past developments with future directions, offering researchers a comprehensive overview and sparking innovation in the field.

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Section: Acoustofluidic Biophysical Therapymentioning

confidence: 86%

Acoustofluidic Actuation of Living Cells

Wu,

Gai,

Zhao

et al. 2024

Micromachines

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“…Although conventional techniques, such as the use of nanoparticles [35] and hydrogel encapsulation [36], work reasonably well in worm immobilization, they still require time-consuming manual operations. In contrast, microfluidic technology provides new possibilities for immobilization by restricting the movement of C. elegans and inhibiting its physiological activities [75][76][77][78][79][80][81][82][83][84][85][86][87][88][89][90]. The automated nature of microfluidic platforms eliminates the need for manual processing, saving time and resources [73,74].…”

Section: Microfluidic Approaches For Immobilizationmentioning

confidence: 99%

“…However, further research is needed to assess the potential impact of this method on the worms' physiology. Similarly, Sridhar et al discovered that surface acoustic waves (SAW) could be used to immobilize worms [90]. This non-contact method enables quick immobilization by exerting SAW acoustic force on the worms through interdigitated electrodes on piezoelectric materials.…”

Section: Microfluidic Approaches For Immobilizationmentioning

confidence: 99%

Worm-Based Diagnosis Combining Microfluidics toward Early Cancer Screening

Shi,

Cui,

Chen

et al. 2024

Micromachines

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Early cancer diagnosis increases therapy efficiency and saves huge medical costs. Traditional blood-based cancer markers and endoscopy procedures demonstrate limited capability in the diagnosis. Reliable, non-invasive, and cost-effective methods are in high demand across the world. Worm-based diagnosis, utilizing the chemosensory neuronal system of C. elegans, emerges as a non-invasive approach for early cancer diagnosis with high sensitivity. It facilitates effectiveness in large-scale cancer screening for the foreseeable future. Here, we review the progress of a unique route of early cancer diagnosis based on the chemosensory neuronal system of C. elegans. We first introduce the basic procedures of the chemotaxis assay of C. elegans: synchronization, behavior assay, immobilization, and counting. Then, we review the progress of each procedure and the various cancer types for which this method has achieved early diagnosis. For each procedure, we list examples of microfluidics technologies that have improved the automation, throughput, and efficiency of each step or module. Finally, we envision that microfluidics technologies combined with the chemotaxis assay of C. elegans can lead to an automated, cost-effective, non-invasive early cancer screening technology, with the development of more mature microfluidic modules as well as systematic integration of functional modules.

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“…27 To date, SAW-based miniaturized devices have been widely used in the manipulation and characterization of biological objects. 5,16,18,[28][29][30][31][32][33][34][35][36][37] The SAW technology has also been an emerging and powerful tool in biomedical fields such as bioanalysis and chemical research. 38 SAW-based microfluidics allows a direct manipulation of liquids and particles suspended in liquids in a strong-gradient acoustic field.…”

Section: Introductionmentioning

confidence: 99%