Sur le rôle métabolique de la proferrorosamine A produite par Pseudomonas roseus fluorescens J. C. MARCHAL 1937

Abstract

La biosynthèse de la proferrorosamine A produite par Pseudomonas roseus fluorescens est inhibée par le tryptophane et cette inhibition est levée par l'acide shikimique. A la suite de l'ensemble des résultats acquis, des hypothèses concernant le rôle métabolique de la proferrorosamine A sont formulées et discutées.

The Fe 2+ Chelator Proferrorosamine A Is Essential for the Siderophore-Mediated Uptake of Iron by Pseudomonas roseus fluorescens

“…The higher the concentration of iron available for the microorganism, the lower the production of both chelating compounds. The decrease in proferrorosamine production as the concentration of iron in the medium increases was reported earlier by Pouteau-Thouvenot et al (19). The production of siderophores, however, is much more sensitive to iron availability than that of proferrorosamine.…”

“…The nitrogen and carbon sources were autoclaved separately. Lasseur's medium was made as described by Pouteau-Thouvenot et al (19). LEDDHA agar is Lasseur's medium without FeSO4 but supplemented with 800 ppm ethylenediaminedihydroxyphenylacetic acid (EDDHA).…”

“…roseusfluorescens. samine phenotype of wild-type P. roseus fluorescens on Lasseur's medium is expressed as pink colonies (19).…”

“…The high affinity for Fe2" is due to the ot-diimine structure of the 2(2'-pyridyl)pyrroline. The production of proferrorosamine A by P. roseus fluorescens is inversely correlated with the concentration of available iron in the medium (19). Proferrorosamine A, however, is not produced only by P. roseus fluorescens; it is also a metabolite of Erwinia rhapontici (9).…”

Isolation of aSerratia marcescensthat Produces a Water-soluble Reddish-violet Peptide Complex

“…This pigment was then characterized as ferropyrimine,8) a pigment first isolated from a strain of Pseudomonas. 9 ) Pyrimine is identical with a compound that have named formerly proferrorosamine A by Viscontini et al 10 ) Another water-soluble reddish-violet pigment, purpuratin, 11) produced by Streptomyces propurpuratus have been reported by Kanda et al and the VIS spectrum is very similar to .that of Z-54 peptide complex, but its specific rotation is very much lower than that of Z-54 peptide complex and the chemical structure of purpuratin is not clear. The VIS spectrum of the Z-54 peptide complex is also close to that of ferropyrimine.…”