Sur la combinaison des isohémagglutinines avec les groupes agglutinogènes

Studies on Agglutination by Macromolecular Antibodies

“…The observation which demonstrated that the test row incubated at 4°C and then re incubated at 37°C shows a similar titer as recorded after primary incubation at 37°C corresponds well to equilibrium studies [4]. The mixture of B erythrocytes with an anti-B serum reaches an equi librium at 4°C which results in some degree of agglutination.…”

Quantitative, Kinetic and Thermodynamic Analysis of Weak B(60) Erythrocyte Phenotypes

“…The difference between the agglutination percentages at equilibrium, at 4°C (P^ax) and 22 °C (P^jp) is expressed by A P Thermodynamic measurements. We used the method described by Filitti-Wurmser et al [8] modified by Salmon and Hautenauve [15] to measure the fixation of the naturally occurring anti-B VIN (AxO) on weak B erythrocytes at two different temperatures, Tx and T2; by plotting max* total antibody N4 Ni free antibody at equilibrium against Nt (total antigen), the slopes of the obtained straight lines were proportional to mK (m=number of B antigenic sites; K = association constant).…”

A(1) Weak B Phenotypes Found in Two Generations of a Romanian Family: A(1)B(x) versus cis AB Complex

“…The fixation of the naturally occurring anti-B (AjO) VIN on the RBC of the three 'A4 weak B' individuals was carried out at 4 and 25 °C in order to determine the standard enthalpy change of the reaction, using the method of Filitti-Wurmser et al [1] and Salmon and Hautenauve [7], but the affinity of the anti-B for this B antigen was too weak to allow this calculation.…”