Supravital fluorometric apoptosis detection in a single mouse embryo using lab-on-a-chip

Walczak, Rafał; Śniadek, Patrycja; Dziuban, Jan; Kluger, Joanna; Soyta, Anna Chełmońska

doi:10.1039/c0lc00667j

Cited by 14 publications

(13 citation statements)

References 21 publications

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“…Furthermore, the combination of the LOC technique and flow cytometry-like methodology allows building an LOC-based system with microchannel dimensions similar to those of the characterized cells, that is, oocytes or embryos [ 28 – 33 ]. Up to the present time, the application of LOC systems in reproductive biology has been published in only a few papers [ 23 , 34 – 37 ]. LOCs can be used to characterize oocytes and embryos using different techniques.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology

Kempisty

Walczak

Antosik

et al. 2014

BioMed Research International

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Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each, n = 100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3–5 mm), and small (<3 mm), were analyzed after preceding in vitro maturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned “face-to-face.” Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P < 0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.

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Section: Introductionmentioning

confidence: 99%

Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology

Kempisty

Walczak

Antosik

et al. 2014

BioMed Research International

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“…Twenty studies characterized oocytes using microfluidics (Table 1). 56,[69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86][87] Microfluidic chips can measure large numbers of oocytes with high throughput and sensitivity. 88 Seven studies deformed oocytes to test their mechanical properties (Figure 4a-d), [69][70][71][72][73][74][75] including one that applied injection force to test oocyte mechanics (Figure 4c), 70 and three that used compression force.…”

Section: Oocyte Evaluationmentioning

confidence: 99%

Microfluidic chip as a promising evaluation method in assisted reproduction: A systematic review

Wu,

Yan

et al. 2023

Bioengineering & Transla Med

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The aim of assisted reproductive technology (ART) is to select the high‐quality sperm, oocytes, and embryos, and finally achieve a successful pregnancy. However, functional evaluation is hindered by intra‐ and inter‐operator variability. Microfluidic chips emerge as the one of the most powerful tools to analyze biological samples for reduced size, precise control, and flexible extension. Herein, a systematic search was conducted in PubMed, Scopus, Web of Science, ScienceDirect, and IEEE Xplore databases until March 2023. We displayed and prospected all detection strategies based on microfluidics in the ART field. After full‐text screening, 71 studies were identified as eligible for inclusion. The percentages of human and mouse studies equaled with 31.5%. The prominent country in terms of publication number was the USA (n = 13). Polydimethylsiloxane (n = 49) and soft lithography (n = 28) were the most commonly used material and fabrication method, respectively. All articles were classified into three types: sperm (n = 38), oocytes (n = 20), and embryos (n = 13). The assessment contents included motility, counting, mechanics, permeability, impedance, secretion, oxygen consumption, and metabolism. Collectively, the microfluidic chip technology facilitates more efficient, accurate, and objective evaluation in ART. It can even be combined with artificial intelligence to assist the daily activities of embryologists. More well‐designed clinical studies and affordable integrated microfluidic chips are needed to validate the safety, efficacy, and reproducibility.Trial registration: The protocol was registered in the Open Science Frame REGISTRIES (identification: osf.io/6rv4a).

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“…For example, typical applications that require both heating and optical performances are the cell and bacteria culture processes [ 101 , 102 , 103 , 104 ]. Indeed, there is an increasing interest in performing robust and efficient on-chip cell cultures, that can in turn led to more elaborated analysis, like quality evaluation, drug resistance, electropermeabilization, and so on [ 105 , 106 , 107 , 108 , 109 ]. The proposed platform can find its application even in this field, since it allows to perform a biological culture and to provide, at the same time, a functioning analysis of the bioanalyte when stimulated by several external agents, like thermal, chemical, or electrical agents stimuli [ 110 ].…”

Section: Evaluation Of System Performancesmentioning

confidence: 99%

Thin-Film-Based Multifunctional System for Optical Detection and Thermal Treatment of Biological Samples

et al. 2022

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In this work, we present a multifunctional Lab-on-Chip (LoC) platform based on hydrogenated amorphous silicon sensors suitable for a wide range of application in the fields of biochemical and food quality control analysis. The proposed system includes a LoC fabricated on a 5 cm × 5 cm glass substrate and a set of electronic boards for controlling the LoC functionalities. The presented Lab-on-Chip comprises light and temperature sensors, a thin film resistor acting as a heating source, and an optional thin film interferential filter suitable for fluorescence analysis. The developed electronics allows to control the thin film heater, a light source for fluorescence and absorption measurements, and the photosensors to acquire luminescent signals. All these modules are enclosed in a black metal box ensuring the portability of the whole platform. System performances have been evaluated in terms of sensor optical performances and thermal control achievements. For optical sensors, we have found a minimum number of detectable photons of 8 × 104 s−1·cm−2: at room temperature, 1.6 × 106 s−1·cm−2: in presence of fluorescence excitation source, and 2.4 × 106 s−1·cm−2· at 90 °C. From a thermal management point of view, we have obtained heating and cooling rates both equal to 2.2 °C/s, and a temperature sensor sensitivity of about 3 mV/°C even in presence of light. The achieved performances demonstrate the possibility to simultaneously use all integrated sensors and actuators, making promising the presented platform for a wide range of application fields.

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Supravital fluorometric apoptosis detection in a single mouse embryo using lab-on-a-chip

Cited by 14 publications

References 21 publications

Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology

Microfluidic Method of Pig Oocyte Quality Assessment in relation to Different Follicular Size Based on Lab-on-Chip Technology

Microfluidic chip as a promising evaluation method in assisted reproduction: A systematic review

Thin-Film-Based Multifunctional System for Optical Detection and Thermal Treatment of Biological Samples

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