Supramolecular self-associating amphiphiles: determination of molecular self-association properties and calculation of critical micelle concentration using a high-throughput, optical density based methodology

Abstract

A microplate reader assay to enable the characterisation of amphiphile self-assembly processes and derivation of the critical micelle concentration (CMC).

Establishing the selective phospholipid membrane coordination, permeation and lysis properties for a series of ‘druggable’ supramolecular self-associating antimicrobial amphiphiles

“…28,38,39 Synthesis SSAs 1-6, 10, 13, and 14 were prepared in line with previously published syntheses. [37][38][39][40][41] SSA 7 was obtained as a white solid in a yield of 91%, through the reaction of tetrabutylammonium (TBA) aminomethane sulfonate with 2-aminobenzothiazole and 1,1 0 -carbonyldiimidazole (CDI). SSAs 8, 9, and 12 were synthesised through the reaction of the appropriate TBA amino-ate salt with the appropriate (belzothiazol-2-yl)aniline and either triphosgene or thiophosgene, and obtained as either a white or yellow solid in yields of 67%, 64% or 28% respectively.…”

Tools to enable the study and translation of supramolecular amphiphiles

“…12, 14 and 15) over a range of temperatures. 108 After first estimating CAC using the pendant drop method at ≈291–298 K, Hiscock and co-workers then ran a series of OD microplate well scans at increasing concentrations and temperatures, and in triplicate to ensure the validity and reproducibility of any data obtained. It was found that CAC values calculated using the OD method were lower than those calculated using the pendant drop method, as shown in Table 3.…”

Controlling the structure of supramolecular fibre formation for benzothiazole based hydrogels with antimicrobial activity against methicillin resistantStaphylococcus aureus

“…SSAs 1-7 were prepared in line with previously published syntheses. 23,29,32 Solid state single crystal X-ray diffraction studies…”