Supramolecular Self‐Assembled DNA Nanosystem for Synergistic Chemical and Gene Regulations on Cancer Cells

Abstract

Incorporating multiple molecular interactions within asystem to realize the metabolic reprogramming of cancer cells is prospected to be of great potential in cancer therapy. Herein, we report asupramolecular self-assembled DNAnanosystem, which reprogrammed the cellular antioxidant system via synergistic chemical and gene regulations.I nt he nanosystem, amphipathic telluroether was coordinated with Mn II to selfassemble into micelle,o nw hich as iNrf2 integrated DNA network was assembled. The great electron-donating capability of telluroether was revealed to greatly promote Mn II -based Fenton-like reaction to generate subversive COH in cancer cells. In response to adenosine triphosphoric acid, the siNrf2 was specially released in cytoplasm for down-regulating expression of detoxification enzymes,w hiche nhanced chemocatalysismediated oxidative stress in cancer cells,t hus significantly suppressing tumor progression.

Nucleic acid-based artificial nanocarriers for gene therapy

“…Reproduced with permission. 93 Copyright 2021, Wiley-VCH. (e) DNA nanotube was assembled with siRNA on the overhangs and the smart key sequence on its hairpin domain as a dual ''lock-and-key'' delivery system (siRNA-ONV).…”

“…3d). 93 This cascade hybridization of DNA hairpins with ATP-triggered siRNA switches could be integrated into polymeric nano-frameworks to achieve precise siRNA delivery. 94 2.1.5.…”

Programmable mismatch-fueled high-efficiency DNA signal amplifier

“…Nevertheless, the CMDJA has also suffered from multiple-step reactions and weak driving forces, resulting in inherent time-consuming and suboptimal kinetic and thermodynamic properties, which further limits its deeper exploitation and wider application. [18][19][20] On account of these disadvantages, it is of critical signicance to improve the reaction speed and efficiency of CMDJAs to advance their superiority and applicability in the areas of nucleic acid signal amplication, biomarker assay, biomolecular nanomachines, and computing. 2,21 Since the performance of CMDJAs that relies on the toehold strand displacement reaction (TSDR) is immensely limited by the branch migration step in the displacement domain, 22,23 the producing rate and yield of a multiple-arm DNA junction would be inuenced by the reaction equilibrium of the step of branch migration in the TSDR.…”