Supramolecular Peptide Assemblies as Antimicrobial Scaffolds

Simonson, Andrew; Aronson, Matthew R.; Medina, Scott H.

doi:10.3390/molecules25122751

Cited by 29 publications

(25 citation statements)

References 232 publications

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“…Most studies of the antimicrobial mechanisms of action of amyloids reveal their destructive effect on cell membranes, for example, through channel/pores formation or membrane thinning [ 12 , 173 , 174 , 175 ]. Several studies have demonstrated the formation of antibacterial nanonets by amyloids, which inhibit the spread of microbes [ 176 , 177 ]. AMPs capable of forming hydrogels with mechanical protective properties for the further proliferation of both planktonic bacterial cells and biofilms are of particular interest [ 178 , 179 ].…”

Section: Mechanisms Of Antimicrobial Action Of Peptidesmentioning

confidence: 99%

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Kurpe

Grishin

Surin

et al. 2020

IJMS

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At present, much attention is paid to the use of antimicrobial peptides (AMPs) of natural and artificial origin to combat pathogens. AMPs have several points that determine their biological activity. We analyzed the structural properties of AMPs, as well as described their mechanism of action and impact on pathogenic bacteria and viruses. Recently published data on the development of new AMP drugs based on a combination of molecular design and genetic engineering approaches are presented. In this article, we have focused on information on the amyloidogenic properties of AMP. This review examines AMP development strategies from the perspective of the current high prevalence of antibiotic-resistant bacteria, and the potential prospects and challenges of using AMPs against infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

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Section: Mechanisms Of Antimicrobial Action Of Peptidesmentioning

confidence: 99%

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Kurpe

Grishin

Surin

et al. 2020

IJMS

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“…Gel formation is initiated by effecting a decrease in solubility to a homogeneous solution of hydrogelator, which can be achieved through a temperature change [ 4 ], pH change [ 5 ] or enzymatic modification [ 6 ]. Peptide hydrogels have found many medicinal applications such as drug delivery vehicles in targeted therapies against cancer and inflammatory diseases [ 7 ], anti-bacterial wound dressings [ 8 ], 3D bioprinting, tissue engineering [ 9 ], skin regeneration, and as extracellular culture medium for the culturing of stem cells and neuronal cells [ 10 ]. The peptide capping groups are usually bi- or tricyclic aromatic groups, such as fluorenylmethoxycarbonyl (Fmoc), 9-anthracenemethoxycarbonyl (Amoc), indole-3-acetyl, naphthoyl or naproxen [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

et al. 2021

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Short peptides capped on the N-terminus with aromatic groups are often able to form supramolecular hydrogels, via self-assembly, in aqueous media. The rheological properties of these readily tunable hydrogels resemble those of the extracellular matrix (ECM) and therefore have potential for various biological applications, such as tissue engineering, biosensors, 3D bioprinting, drug delivery systems and wound dressings. We herein report a new photo-responsive supramolecular hydrogel based on a “caged” dehydropeptide (CNB-Phe-ΔPhe-OH 2), containing a photo-cleavable carboxy-2-nitrobenzyl (CNB) group. We have characterized this hydrogel using a range of techniques. Irradiation with UV light cleaves the pendant aromatic capping group, to liberate the corresponding uncaged model dehydropeptide (H-Phe-ΔPhe-OH 3), a process which was investigated by 1H NMR and HPLC studies. Crucially, this cleavage of the capping group is accompanied by dissolution of the hydrogel (studied visually and by fluorescence spectroscopy), as the delicate balance of intramolecular interactions within the hydrogel structure is disrupted. Hydrogels which can be disassembled non-invasively with temporal and spatial control have great potential for specialized on-demand drug release systems, wound dressing materials and various topical treatments. Both 2 and 3 were found to be non-cytotoxic to the human keratinocyte cell line, HaCaT. The UV-responsive hydrogel system reported here is complementary to previously reported related UV-responsive systems, which are generally composed of peptides formed from canonical amino acids, which are susceptible to enzymatic proteolysis in vivo. This system is based on a dehydrodipeptide structure which is known to confer proteolytic resistance. We have investigated the ability of the photo-activated system to accelerate the release of the antibiotic, ciprofloxacin, as well as some other small model drug compounds. We have also conducted some initial studies towards skin-related applications. Moreover, this model system could potentially be adapted for on-demand “self-delivery”, through the uncaging of known biologically active dehydrodipeptides.

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“… 25,30 Here, we discuss peptides because they are ideal for liposomal surface modification due to their ease of synthesis, ease of manufacturing at industrial and clinical levels, and chemical versatility. 47–49 Overall, these advantageous characteristics promote an immense field for novel innovation and discovery for a plethora of diseases. In this review, we focus on the recent advancements in receptor targeting peptide-functionalized liposomes over the past five years.…”

Section: Introductionmentioning

confidence: 99%

Peptide functionalized liposomes for receptor targeted cancer therapy

2021

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Most clinically approved cancer therapies are potent and toxic small molecules that are limited by severe off-target toxicities and poor tumor-specific localization. Over the past few decades, attempts have been made to load chemotherapies into liposomes, which act to deliver the therapeutic agent directly to the tumor. Although liposomal encapsulation has been shown to decrease toxicity in human patients, reliance on passive targeting via the enhanced permeability and retention (EPR) effect has left some of these issues unresolved. Recently, investigations into modifying the surface of liposomes via covalent and/or electrostatic functionalization have offered mechanisms for tumor homing and subsequently controlled chemotherapeutic delivery. A wide variety of biomolecules can be utilized to functionalize liposomes such as proteins, carbohydrates, and nucleic acids, which enable multiple directions for cancer cell localization. Importantly, when nanoparticles are modified with such molecules, care must be taken as not to inactivate or denature the ligand. Peptides, which are small proteins with <30 amino acids, have demonstrated the exceptional ability to act as ligands for transmembrane protein receptors overexpressed in many tumor phenotypes. Exploring this strategy offers a method in tumor targeting for cancers such as glioblastoma multiforme, pancreatic, lung, and breast based on the manifold of receptors overexpressed on various tumor cell populations. In this review, we offer a comprehensive summary of peptide-functionalized liposomes for receptor-targeted cancer therapy.

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Supramolecular Peptide Assemblies as Antimicrobial Scaffolds

Cited by 29 publications

References 232 publications

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Antimicrobial and Amyloidogenic Activity of Peptides. Can Antimicrobial Peptides Be Used against SARS-CoV-2?

Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

Peptide functionalized liposomes for receptor targeted cancer therapy

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