Supramolecular Heterodimer Peptides Assembly for Nanoparticles Functionalization

Abstract

Nanoparticle (NP) surface functionalization with proteins, including monoclonal antibodies (mAbs), mAb fragments, and various peptides, has emerged as a promising strategy to enhance tumor targeting specificity and immune cell interaction. However, these methods often rely on complex chemistry and suffer from batch‐dependent outcomes, primarily due to limited control over the protein orientation and quantity on NP surfaces. To address these challenges, we present a novel approach based on the supramolecular assembly of two peptides to create a heterotetramer displaying VHHs on NP surfaces. This approach effectively targets both tumor‐associated antigens (TAAs) and immune cell‐associated antigens. In vitro experiments showcased its versatility, as we biofunctionalized various NP types, including liposomes, PLGA NPs, and ultrasmall silica‐based NPs, and evaluated the VHHs targeting of known TAAs (HER2 for breast cancer, CD38 for multiple myeloma) and an immune cell antigen (NKG2D for natural killer (NK) cells). In in vivo studies using a HER2+ breast cancer mouse model, our approach demonstrated enhanced tumor uptake, retention, and penetration compared to the behavior of nontargeted analogs, affirming its potential for diverse applications.This article is protected by copyright. All rights reserved

Identification of a linear B-cell epitope on the “puff” loop of the Senecavirus A VP2 protein involved in receptor binding

“…Monoclonal antibodies with high specificity and sensitivity are widely used in the diagnosis and treatment of diseases, such as Alzheimer's disease (AD), have produced encouraging cognitive and clinical results (Qiao et al, 2024 ). Besides, engineering modifications of mAbs significantly improve their therapeutic properties, which has emerged as a promising strategy to enhance tumor targeting specificity and immune cell interactions (Mathieu et al, 2024 ). In addition, mAbs are widely applied for the establishment of immunological detection methods for infectious or non-infectious diseases.…”