Supramolecular Assemblies of Glycopeptides as Mimics of the Extracellular Matrix

Costa, Diana Soares da; Brito, Alexandra; Reis, Rui L.; Pashkuleva, Iva

doi:10.1002/9783527825820.ch7

Cited by 1 publication

(2 citation statements)

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“…29,30 Injectable peptide hydrogels have been used in a variety of immunotherapeutic and infectious disease applications 31−33 and have been previously combined with carbohydrates to make supramolecular materials that mimic the extracellular matrix. 34,35 Self-assembling peptide hydrogels have also shown promise as immunotherapeutic agents for cancer and infectious disease due to their ability to act as adjuvants and to control the release of immunomodulating payloads. 36−39 Multidomain peptides (MDPs) are a class of self-assembling peptides that use a sequence of alternating polar and nonpolar residues flanked by charged residues to favor β-sheet formation while providing the molecular frustration necessary to form a self-supporting fibrous hydrogel.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Peptide-based hydrogels are of clinical interest due to their modular bioactivity, ability to control the release of a variety of payloads, and shear thinning and self-healing properties that allow for injection through small-bore needles. , Injectable peptide hydrogels have been used in a variety of immunotherapeutic and infectious disease applications − and have been previously combined with carbohydrates to make supramolecular materials that mimic the extracellular matrix. , Self-assembling peptide hydrogels have also shown promise as immunotherapeutic agents for cancer and infectious disease due to their ability to act as adjuvants and to control the release of immunomodulating payloads. − …”

Section: Introductionmentioning

confidence: 99%

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Dynamic Imine Bonding Facilitates Mannan Release from a Nanofibrous Peptide Hydrogel

et al. 2022

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Recently, there has been increased interest in using mannan as an immunomodulatory bioconjugate. Despite notable immunological and functional differences between the reduced (R-Man) and oxidized (O-Man) forms of mannan, little is known about the impact of mannan oxidation state on its in vivo persistence or its potential controlled release from biomaterials that may improve immunotherapeutic or prophylactic efficacy. Here, we investigate the impact of oxidation state on the in vitro and in vivo release of mannan from a biocompatible and immunostimulatory multidomain peptide hydrogel, K 2 (SL) 6 K 2 (abbreviated as K 2 ), that has been previously used for the controlled release of protein and small molecule payloads. We observed that O-Man released more slowly from K 2 hydrogels in vitro than R-Man. In vivo, the clearance of O-Man from K 2 hydrogels was slower than O-Man alone. We attributed the slower release rate to the formation of dynamic imine bonds between reactive aldehyde groups on O-Man and the lysine residues on K 2 . This imine interaction was also observed to improve K 2 + O-Man hydrogel strength and shear recovery without significantly influencing secondary structure or peptide nanofiber formation. There were no observed differences in the in vivo release rates of O-Man loaded in K 2 , R-Man loaded in K 2 , and R-Man alone. These data suggest that, after subcutaneous injection, R-Man naturally persists longer in vivo than O-Man and minimally interacts with the peptide hydrogel. These results highlight a potentially critical, but previously unreported, difference in the in vivo behavior of O-Man and R-Man and demonstrate that K 2 can be used to normalize the release of O-Man to that of R-Man. Further, since K 2 itself is an adjuvant, a combination of O-Man and K 2 could be used to enhance the immunostimulatory effects of O-Man for applications such as infectious disease vaccines and cancer immunotherapy.

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