Supramolecular Assemblies for Transporting Proteins Across an Immiscible Solvent Interface

Gao, Jingjing; Zhao, Bo; Wang, Meizhe; Serrano, Mahalia A. C.; Zhuang, Jiaming; Ray, Moumita; Rotello, Vincent M.; Vachet, Richard W.; Thayumanavan, S.

doi:10.1021/jacs.7b13245

Cited by 26 publications

(25 citation statements)

References 27 publications

(40 reference statements)

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“…Interestingly, it has been proven that some enzymes such as, α-chymotrypsin (Moyano et al, 2010), lipases (Maiti et al, 2011) or horseradish peroxidase (Zhong et al, 2016) show enhanced catalytic properties when they are confined in reverse micelles. It has been proposed that this positive effect could be a consequence of the conformational changes suffered by the protein, the high concentration of substrates within the RM, or an altered hydration state of the active site of the enzyme (Moyano et al, 2010;Sintra et al, 2014;Gao et al, 2018). However, the principles behind the so-called enzyme "superactivity" have not been elucidated yet.…”

Section: Reverse Micellesmentioning

confidence: 99%

Tunable Polymeric Scaffolds for Enzyme Immobilization

Rodriguez‐Abetxuko

Sánchez-deAlcázar

Muñumer

et al. 2020

Front. Bioeng. Biotechnol.

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The number of methodologies for the immobilization of enzymes using polymeric supports is continuously growing due to the developments in the fields of biotechnology, polymer chemistry, and nanotechnology in the last years. Despite being excellent catalysts, enzymes are very sensitive molecules and can undergo denaturation beyond their natural environment. For overcoming this issue, polymer chemistry offers a wealth of opportunities for the successful combination of enzymes with versatile natural or synthetic polymers. The fabrication of functional, stable, and robust biocatalytic hybrid materials (nanoparticles, capsules, hydrogels, or films) has been proven advantageous for several applications such as biomedicine, organic synthesis, biosensing, and bioremediation. In this review, supported with recent examples of enzyme-protein hybrids, we provide an overview of the methods used to combine both macromolecules, as well as the future directions and the main challenges that are currently being tackled in this field.

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Section: Reverse Micellesmentioning

confidence: 99%

Tunable Polymeric Scaffolds for Enzyme Immobilization

Rodriguez‐Abetxuko

Sánchez-deAlcázar

Muñumer

et al. 2020

Front. Bioeng. Biotechnol.

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“…Non-covalent polymer-protein binding studies, sometimes involvingd elivery experiments, is ab road field, and additional reports relatedt ot his topic that highlight various binding interactions and characterization methods can be found in Refs. [58][59][60][61][62][63][64][65][66][67][68][69][70][71].B inding studies showcasing availablet echniques could potentially contain criticali nformation necessary for future protein-delivery optimization studies and should not be overlooked.…”

Section: Other Non-covalent Protein-basedcomplexes For Deliverymentioning

confidence: 99%

Associative and Dissociative Processes in Non‐Covalent Polymer‐Mediated Intracellular Protein Delivery

Posey

Tew

2018

Chemistry An Asian Journal

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Functional protein delivery has created new opportunities for studying intracellular processes and discovering new therapeutics. To that end, researchers have pursued intracellular protein delivery by using an increasing number of methods. This focus review will highlight polymeric carriers that non-covalently bind and deliver protein cargo in vitro. The correlation between polymer-protein binding and delivery as well as the correlation between complex-membrane binding and delivery is reviewed. Finally, binding and its relation to the intracellular function of the protein post-delivery is considered. The purpose of this review is to evaluate the role that binding interactions play in the non-covalent protein-delivery landscape. Presently, the literature does not adequately resolve how binding throughout the process ultimately affects delivery. The field does contain preliminary insights that are expected to impact future delivery applications when developed further.

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“…1,2 Inspired from biological macromolecules, researchers have developed a variety of functional synthetic molecules based on amphiphilic building blocks. [3][4][5][6] The design of such molecules mainly involves a linear hydrophobic tail tethered to an ionic head group, that can form micellar assemblies in aqueous medium. [7][8][9] Such assemblies are used in various applications such as surfactants, vesicles, and as transporters of biological cargo.…”

Section: Introductionmentioning

confidence: 99%

Amphiphilic Small Molecule Assemblies to Enhance the Solubility and Stability of Hydrophobic Drugs

Gangarde¹,

Sajeev²,

Panigrahi³

et al. 2020

Preprint

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We report the synthesis of gemini-type amphiphilic molecules that form stable assemblies in aqueous medium. The assembly property of molecule M2 in aqueous solution was first inferred from peak broadening observed in the proton NMR spectrum. This was supported by dynamic light scattering and transmission electron microscopy analysis. The assembly formed from M2 (M2agg) was used to solubilize the hydrophobic drugs curcumin and doxorubicin at physiological pH. M2agg was able to effectively solubilize curcumin as well as protect it from degradation under UV irradiation. Upon solubilization in M2agg, curcumin showed excellent cell permeability, and higher toxicity to cancer cells over normal cells, likely due to enhanced cellular uptake and increased stability. M2agg also showed pH-dependent release of doxorubicin, resulting in controlled toxicity on cancer cell lines, making it a promising candidate for the selective delivery of drugs to cancer cells.

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Supramolecular Assemblies for Transporting Proteins Across an Immiscible Solvent Interface

Cited by 26 publications

References 27 publications

Tunable Polymeric Scaffolds for Enzyme Immobilization

Tunable Polymeric Scaffolds for Enzyme Immobilization

Associative and Dissociative Processes in Non‐Covalent Polymer‐Mediated Intracellular Protein Delivery

Amphiphilic Small Molecule Assemblies to Enhance the Solubility and Stability of Hydrophobic Drugs

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