Supra‐Fluorophores: Ultrabright Fluorescent Supramolecular Assemblies Derived from Conventional Fluorophores in Water

Abstract

Fluorescent organic nanoparticles (NPs) with exceptional brightness hold significant promise for demanding fluorescence bioimaging applications. Although considerable efforts have been invested in developing novel organic dyes with enhanced performance, augmenting the brightness of conventional fluorophores is still one of the biggest challenges to overcome. In this study, we present a supramolecular strategy for constructing ultrabright fluorescent nanoparticles in aqueous media (referred to as “Supra‐fluorophores”) derived from conventional fluorophores. To achieve this, we have employed a cylindrical nanoparticle with a hydrophobic microdomain, assembled by a cyclic peptide‐diblock copolymer conjugate in water, as a supramolecular scaffold. The noncovalent dispersion of fluorophore moieties within the hydrophobic microdomain of the scaffold effectively mitigates the undesired aggregation‐caused quenching and fluorescence quenching by water, resulting in fluorescent NPs with high brightness. This strategy is applicable to a broad spectrum of fluorophore families, covering polyaromatic hydrocarbons, coumarins, boron‐dipyrromethenes, cyanines, xanthenes, and squaraines. The resulting fluorescent NPs demonstrate high fluorescence quantum yield (>30%) and brightness per volume (as high as 12 060 M−1 cm−1 nm−3). Moreover, high‐performance NPs with emission in the NIR region are constructed, showcasing up to 20‐fold increase in both brightness and photostability. Our Supra‐fluorophore strategy offers a versatile and effective method for transforming existing fluorophores into ultrabright fluorescent NPs in aqueous environments, for applications such as bioimaging.This article is protected by copyright. All rights reserved