Suppressors of<i>zyg-1</i>Define Regulators of Centrosome Duplication and Nuclear Association in<i>Caenorhabditis elegans</i>

Kemp, Catherine A; Song, Mi Hye; Addepalli, Murali Krishna; Hunter, Ginger L.; O’Connell, Kevin P.

doi:10.1534/genetics.107.071803

Cited by 46 publications

(102 citation statements)

References 53 publications

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“…Overall, we conclude that SPD-2, ZYG-1, SAS-6 and SAS-4 are required for centrosome duplication in proliferating GCs, as anticipated from the sterility associated with spd-2(oj29) and zyg-1(it25) (O'Connell et al, 1998;Kemp et al, 2007), as well as in sas-6(ok2554) and sas-4(tm3951) (Kitagawa et al, 2011).…”

Section: Centriolar Proteins Required For Centrosome Duplication In Tsupporting

confidence: 61%

“…The following genotypes were used: sas-6(ok2554) (Kitagawa et al, 2011), zyg-1(it25) (Kemp et al, 2007), spd-2(oj29) (O'Connell et al, 1998), sas-5(t2033) , sas-5(gk400) (http://www.wormbase.org), sas-4(tm3951) (Kitagawa et al, 2011), cki-2(ok2105) (Buck et al, 2009), fog-1(q253) (Barton and Kimble, 1990) (shifted to the restrictive temperature of 25°C as L1/L2), fog-2(q71) (Schedl and Kimble, 1988), fog-3(q470) (Ellis and Kimble, 1994), tra-1(e1076) (Schedl et al, 1989), her-1(hv1y101) unc-42 and her-1(hv1y101) unc-42 dpy-11;lon-2 (Francis et al, 1995b), analyzed as described (Bean et al, 2004), mog-1(q233) (Graham and Kimble, 1993), fem-3(q96gf) (Barton et al, 1987), cgh-1(ok492) (Audhya et al, 2005), glo-1(zu391) (Hermann et al, 2005), rrf-1(pk1417) (Sijen et al, 2001), GFP-SAS-4 (Kirkham et al, 2003), GFP-SAS-5 , GFP-SAS-6 , GFP-SPD-2 (Kemp et al, 2004), GFP-TBG-1 (Oegema et al, 2001), GFP -TAC-1 (Bellanger and Gönczy, 2003), VIT-2-GFP (Grant and Hirsh, 1999), NMY-2-GFP (Nance et al, 2003).…”

Section: Nematodes and Rna Interference (Rnai)mentioning

confidence: 99%

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Analysis of centriole elimination duringC. elegansoogenesis

et al. 2012

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SUMMARYCentrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation and thus faithful chromosome segregation. In the germ line of most metazoan species, centrioles are maintained during spermatogenesis, but eliminated during oogenesis. Such differential behavior ensures that the appropriate number of centrioles is present in the newly fertilized zygote. Despite being a fundamental feature of sexual reproduction in metazoans, the mechanisms governing centriole elimination during oogenesis are poorly understood. Here, we investigate this question in C. elegans. Using antibodies directed against centriolar components and serial-section electron microscopy, we establish that centrioles are eliminated during the diplotene stage of the meiotic cell cycle. Moreover, we show that centriole elimination is delayed upon depletion of the helicase CGH-1. We also find that somatic cells make a minor contribution to this process, and demonstrate that the germ cell karyotype is important for timely centriole elimination. These findings set the stage for a mechanistic dissection of centriole elimination in a metazoan organism.

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Section: Centriolar Proteins Required For Centrosome Duplication In Tsupporting

confidence: 61%

Section: Nematodes and Rna Interference (Rnai)mentioning

confidence: 99%

Analysis of centriole elimination duringC. elegansoogenesis

et al. 2012

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“…Methods for 4D-DIC microscopy, indirect immunofluorescent staining and spinning disk confocal microscopy have been described previously (Kemp et al, 2007). For 4D-DIC, embryos or spermatocytes were mounted on a Zeiss Axiovert 200M microscope equipped with a Hamamatsu ORCA-ER camera.…”

Section: Cytologymentioning

confidence: 99%

“…For immunostaining, the following antibodies were used at 1:1000 dilutions: DM1A, an anti--tubulin monoclonal antibody (Sigma-Aldrich); 969LA, an affinitypurified rabbit anti-SPD-2 polyclonal antibody (Kemp et al, 2004); -ZYG-1 , an affinity-purified rabbit polyclonal antibody (Kemp et al, 2007); -SPD-5 (Hamill et al, 2002); Alexa Fluor 488 goat anti-rabbit; and 568 goat anti-mouse secondary antibodies (Invitrogen).…”

Section: Cytologymentioning

confidence: 99%

Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1

Peters¹,

Perez²,

Song³

et al. 2010

Journal of Cell Science

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SummaryCentriole duplication is of crucial importance during both mitotic and male meiotic divisions, but it is currently not known whether this process is regulated differently during the two modes of division. In Caenorhabditis elegans, the kinase ZYG-1 plays an essential role in both mitotic and meiotic centriole duplication. We have found that the C-terminus of ZYG-1 is necessary and sufficient for targeting to centrosomes and is important for differentiating mitotic and meiotic centriole duplication. Small truncations of the Cterminus dramatically lower the level of ZYG-1 at mitotic centrosomes but have little effect on the level of ZYG-1 at meiotic centrosomes. Interestingly, truncation of ZYG-1 blocks centrosome duplication in the mitotic cycle but leads to centrosome amplification in the meiotic cycle. Meiotic centriole amplification appears to result from the overduplication of centrioles during meiosis I and leads to the formation of multipolar meiosis II spindles. The extra centrioles also disrupt spermatogenesis by inducing the formation of supernumerary fertilization-competent spermatids that contain abnormal numbers of chromosomes and centrioles. Our data reveal differences in the regulation of mitotic and meiotic centrosome duplication, particularly with regard to ZYG-1 activity, and reveal an important role for centrosomes in spermatid formation.

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“…It is crucial for the duplication of centrosomes and spindle pole bodies (SPB) at NE, and formation anchorage of the chromosome bouquet in mitosis. The LINC complex is also important for spanning the width of the nuclear periplasm (approximately 30 to 50 nm) (Chikashige et al, 2006;Crisp et al, 2006;Tomita and Cooper, 2006;Tzur et al, 2006;Chi et al, 2007;Kemp et al, 2007;Starr, 2009;. Some INM proteins take part in biochemical signaling in the nucleus.…”

Section: Introductionmentioning

confidence: 99%

AtNEA1-identification and characterization of a novel plant nuclear envelope associated protein

Lu¹

2014

Afr. J. Biotechnol.

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In animal and yeast cells, a cross nuclear envelope structure linker of nucleoskeleton and cytoskeleton (LINC) is formed by outer nuclear membrane SUN proteins and inner nuclear membrane KASH proteins. However, little information was acquired about plant SUN-KASH structure until they were found in plant SUN proteins in 2010 and KASH proteins in 2012. The SUN-KASH complex alongside with actin in the microfilament cytoskeleton and nucleaoskeleton together shape the main cell skeleton structure and involve in many important biological functions including cell structure stability, cell movement and cell division. In searching of other plant nuclear envelope associated proteins, arabidopsis nuclear envelop associated (AtNEA1) protein 1, a plant nucleoplasmic protein, was identified from biological studies. AtNEA1 was predicted to have a nuclear localisation signal (NLS), two coiled coil domains and one transmembrane (TM) domain. The mutants with the deletion of respective putative domains were observed under confocal microscopy. The subcellular localisation of mutants implied that putative NLS is not essential for AtNEA1 to diffuse through NPC but can strongly increase the efficiency, both coiled coil domains participate in the interaction of AtNEA1 with its unknown INM intrinsic interaction partner, and putative TM appeared to be non-functional. The function of AtNEA1 in plant was studied through observing tDNA lines.

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Suppressors ofzyg-1Define Regulators of Centrosome Duplication and Nuclear Association inCaenorhabditis elegans

Cited by 46 publications

References 53 publications

Analysis of centriole elimination duringC. elegansoogenesis

Analysis of centriole elimination duringC. elegansoogenesis

Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1

AtNEA1-identification and characterization of a novel plant nuclear envelope associated protein

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