Suppressors of cytokine signaling proteins as modulators of development and innate immunity of insects

Abbas, Muhammad Nadeem; Kausar, Saima; Zhao, Erhu; Cui, Hongjuan

doi:10.1016/j.dci.2019.103561

Cited by 26 publications

(16 citation statements)

References 83 publications

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“…Pc-cathepsin D-like expression profiles are consistent with the previous studies, although the time exposed to the microbial pathogen is different. A recent study suggested that recognition and regulator genes are usually enhanced after microbial infection (Xiong et al, 2015;Abbas et al, 2020a;Abbas et al, 2020b). Thus, we proposed that Pc-cathepsin D-like might implicate in the regulation of immune genes in the P.…”

Section: R a F Tmentioning

confidence: 84%

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Huang

et al. 2021

Genome

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Aspartic proteinases are one of the four families of proteinase enzymes that are widely present in living organisms. They are involved in various physiological events, such as protein degradation, development, and host defense. However, the characterization and functional roles of aspartic proteinases remain to be elucidated in crustaceans. Here, we characterized a fragment of cathepsin D-like cDNA from red swamp crayfish, Procambarus clarkii (Pc-cathepsin D-like). The open reading frame of the Pc-cathepsin D-like gene contained 1152 bp, encoding a protein of 383 amino acid residues. We also evaluated the immunological role of the Pc-cathepsin D-like gene in vivo. Spatial distribution analysis revealed that the Pc-cathepsin D-like mRNA was high in the hepatopancreas, followed by the gut, gills, and hemocytes of P. clarkii. The expression levels of the Pc-cathepsin D-like gene increased following challenge with viral (polyinosinic: polycytidylic acid) and bacterial (lipopolysaccharides, peptidoglycan) PAMPs compared with PBS injection. The suppression of the Pc-cathepsin D-like gene by RNA interference significantly increased the expression of immune-associated genes. These results showed that the Pc-cathepsin D-like gene has an essential biological role in innate immune responses because it regulates the expression of immune-associated genes.

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Section: R a F Tmentioning

confidence: 84%

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Huang

et al. 2021

Genome

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“…We observed DEGs with functions in immunity pathways, including pattern recognition receptors such as C-type lectins (AAEL000543, AAEL014382, AAEL012353, AAEL026265), proteins with a peptidoglycan recognition protein domain (AAEL009474, AAEL019745), a gram-negative binding protein (GNBP) (AAEL007626), and macroglobulin (AAEL001794) which can complex with leucine-rich repeat containing proteins (AAEL001414) [ 58 , 59 ]. MAG extract injection resulted in up-regulation of AAEL019728, a suppressor of cytokine signaling (SOCS) associated with Janus kinases/Signal transducer and activator of transcription protein (JAK/STAT) pathways [ 73 ] and cecropins (AAEL029038, AAEL029044). Transcript levels were also altered for 11 genes with a predicted serine protease domain, which is involved in regulatory control of multiple cellular functions [ 58 , 62 , 64 , 65 ].…”

Section: Resultsmentioning

confidence: 99%

“…aegypti homolog of TEP1 as a DEG responsive to MAG extract injection, we did observe a macroglobulin/complement gene to be differentially regulated by MAG extract (AAEL001794). It will be interesting to investigate the immune genes influenced by SFPs uncovered here for roles in reproduction, particularly the Suppressor of Cytokine Signaling (SOCS) gene’s involvement in JAK/STAT immune signaling pathways [ 73 ].…”

Section: Resultsmentioning

confidence: 99%

Seminal fluid proteins induce transcriptome changes in the Aedes aegypti female lower reproductive tract

et al. 2021

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Background Mating induces behavioral and physiological changes in the arbovirus vector Aedes aegypti, including stimulation of egg development and oviposition, increased survival, and reluctance to re-mate with subsequent males. Transferred seminal fluid proteins and peptides derived from the male accessory glands induce these changes, though the mechanism by which they do this is not known. Results To determine transcriptome changes induced by seminal proteins, we injected extract from male accessory glands and seminal vesicles (MAG extract) into females and examined female lower reproductive tract (LRT) transcriptomes 24 h later, relative to non-injected controls. MAG extract induced 87 transcript-level changes, 31 of which were also seen in a previous study of the LRT 24 h after a natural mating, including 15 genes with transcript-level changes similarly observed in the spermathecae of mated females. The differentially-regulated genes are involved in diverse molecular processes, including immunity, proteolysis, neuronal function, transcription control, or contain predicted small-molecule binding and transport domains. Conclusions Our results reveal that seminal fluid proteins, specifically, can induce gene expression responses after mating and identify gene targets to further investigate for roles in post-mating responses and potential use in vector control.

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“…Serine protease inhibitors and hemolymph protease control the melanization process of the hemolymph to attenuate pathogens [ 39 ]. The peptidoglycan-recognition protein has a strong affinity for Gram-positive bacteria and can also bind with Gram-negative bacteria to initiate signaling via IMD and the Toll pathway [ 49 ], whereas the suppressor of cytokine 2 is a well-known negative regulator of the JAK-STAT pathway, which checks the activity of this pathway during infection and development [ 50 , 51 , 52 , 53 ]. These pathways mainly attenuate microbial infection via inducing the production of antimicrobial peptides [ 39 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing Highlights the Regulatory Role of DNA Methylation in Immune-Related Genes’ Expression of Chinese Oak Silkworm, Antheraea pernyi

Kausar

Liu

Gul

et al. 2022

Insects

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Antheraea pernyi is an important lepidopteran used as a model insect species to investigate immune responses, development, and metabolism modulation. DNA methylation has recently been found to control various physiological processes throughout the life of animals; however, DNA methylation and its effect on the physiology of insects have been poorly investigated so far. In the present study, to better understand DNA methylation and its biological role in the immune system, we analyzed transcriptome profiles of A. pernyi pupae following DNA methylation inhibitor injection and Gram-positive bacteria stimulation. We then compared the profiles with a control group. We identified a total of 55,131 unigenes from the RNA sequence data. A comparison of unigene expression profiles showed that a total of 680 were up-regulated and 631 unigenes were down-regulated in the DNA-methylation-inhibition-bacteria-infected group compared to the control group (only bacteria-injected pupae), respectively. Here, we focused on the immune-related differentially expressed genes (DEGs) and screened 10 genes that contribute to immune responses with an up-regulation trend, suggesting that microbial pathogens evade host immunity by increasing DNA methylation of the host genome. Furthermore, several other unigenes related to other pathways were also changed, as shown in the KEGG analysis. Taken together, our data revealed that DNA methylation seems to play a crucial biological role in the regulation of gene expression in insects, and that infection may enhance the host genome DNA methylation by a yet-unknown mechanism.

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Suppressors of cytokine signaling proteins as modulators of development and innate immunity of insects

Cited by 26 publications

References 83 publications

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Seminal fluid proteins induce transcriptome changes in the Aedes aegypti female lower reproductive tract

Transcriptome Sequencing Highlights the Regulatory Role of DNA Methylation in Immune-Related Genes’ Expression of Chinese Oak Silkworm, Antheraea pernyi

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