Suppressors suaC 109 and suaA 101 of Aspergillus nidulans alter the ribosomal phenotype in vitro

Zamir, A.; Martinelli, S. S.

doi:10.1007/bf01122127

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Bioscience Reports

1987

DOI: 10.1007/bf01122127

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Suppressors suaC 109 and suaA 101 of Aspergillus nidulans alter the ribosomal phenotype in vitro

A. Zamir

S. S. Martinelli

Abstract: A new homologous, cell-free system for protein synthesis has been devised for use with ribosomes and elongation factors from Aspergillus nidulans. Ribosome preparations from strains with either the suaA101 or suaC109 mutations have a higher misreading ratio (non-cognate:cognate amino acid incorporation) in the presence of hygromycin than controls. They can be classed as fidelity mutants. These results also prove that the mutations must be in genes coding for ribosomal proteins or enzymes which modify ribosomal… Show more

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Cited by 5 publications

References 17 publications

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Antisuppressor mutations reduce misreading of the genetic code in Aspergillus nidulans

Martinelli

Perera

1991

Curr Genet

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Antisuppressor mutations were isolated in a strain containing the omnipotent suppressor suaC109. The antisuppressors reduce the activity of translational suppressors in vivo and counteract most aspects of the pleiotropic phenotype associated with the suaC and the suaA suppressor mutations. Using an homologous system for cell-free translation, we have measured translational accuracy in two antisuppressor strains with the genotype suaC109 and either the asuB11 or the asuD14 antisuppressor mutation. Ribosomes from antisuppressor mutants have higher levels of translation accuracy than those from the suppressor strain (suaC109, asu+). Mistranslation levels depended solely on the source of the sucrose-cleaned ribosomes. However, the increased accuracy associated with sucrose-cleaned ribosomes from antisuppressor strains can be nullified by salt-washing, suggesting that the component responsible can be washed off.

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Antisuppressor mutations reduce misreading of the genetic code in Aspergillus nidulans

Martinelli

Perera

1991

Curr Genet

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Hygromcyin- and paromonycin-resistant mutants of Aspergillus nidulans alter translational fidelity

Martinelli

Sheikh

1991

Curr Genet

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Mutants of Aspergillus nidulans resistant to the aminoglycoside antibiotics paromomycin and hygromycin B have been isolated and their growth characteristics are described here. Most paromomycin mutants were cross-resistant to hygromycin and geneticin. All the hygromycin-resistant mutants were slightly cross-resistant to geneticin. Out of the 15 mutants tested 14 had drug-resistant ribosomes in vitro and all 12 of those investigated further had reduced levels of translational misreading. Five new loci have been found--parA on linkage group I, hygA on III, hygB on IV, hygC on V, hygD on VI and parB on VIII. This increases, to at least 12, the number of translational fidelity loci in A. nidulans.

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Ammonium ion sensitivity is a ribosomal phenotype associated with suppressor mutations in the suaC gene of Aspergillus nidulans

1988

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Ammonium ions are selectively toxic to strains containing mutations in the suaC gene which can mutate to a suppressor phenotype. This phenotype is associated with increased ribosomal misreading in vitro (Zamir and Martinelli 1987) and altered ribosomal proteins (Harvey and Martinelli 1983). Such ammonium-sensitivity is a feature of both strong and weak suppressor alleles, and segregates with suppressor ability in crosses. Suppressor mutations in the suaB and suaD genes are not affected, nor are those in suaA, another ribosomal suppressor gene. Thus, the ammonium-effect is locus specific. Mutations which act as anti-suppressors (asu-) of suppressor suaC109 also partially reverse the ammonium ion sensitivity associated with this mutation. This effect is in line with their restoration of other aspects of the pleiotropic phenotype to normal. The cations, lithium and rubidium, mimick the effects of ammonium ions. Only ribosomes from suaC strains are sensitive to the presence of NH+4 ions in vitro.

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Suppressors suaC 109 and suaA 101 of Aspergillus nidulans alter the ribosomal phenotype in vitro

Cited by 5 publications

References 17 publications

Antisuppressor mutations reduce misreading of the genetic code in Aspergillus nidulans

Antisuppressor mutations reduce misreading of the genetic code in Aspergillus nidulans

Hygromcyin- and paromonycin-resistant mutants of Aspergillus nidulans alter translational fidelity

Ammonium ion sensitivity is a ribosomal phenotype associated with suppressor mutations in the suaC gene of Aspergillus nidulans

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