Suppressor of RNA silencing encoded by Beet yellows virus

Reed, Jonathan C.; Kasschau, Kristin D.; Prokhnevsky, Alexey I.; Gopinath, K.; Pogue, Gregory P.; Carrington, James C.; Dolja, Valerian V.

doi:10.1016/s0042-6822(02)00051-x

Cited by 132 publications

(80 citation statements)

References 31 publications

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“…For example, the potyviral HcPro most likely inhibits unwinding of the siRNA duplex and RISC assembly (10), P25 of Potato virus X suppresses the production or accumulation of mobile silencing signals (15), and P19 of Tomato bushy stunt virus (TBSV) and P21 of Beet yellow virus inhibit RNA silencing by physically interacting with siRNAs and preventing their processing or incorporation into the RISC (9,10,(16)(17)(18)(19)(20). However, among numerous known viral RNA-silencing suppressors, only two, the 2b protein of Cucumber mosaic virus (CMV) and poleroviral (P0), have been shown to interact directly with a protein component of the host-silencing pathway.…”

Section: P Lants Have Evolved An Innate Immune Response To Invadingmentioning

confidence: 99%

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Glick

Zrachya

Levy

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

272

202

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The V2 protein of tomato yellow leaf curl geminivirus (TYLCV) functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of V2, however, remains unknown. Here we show that V2 interacts directly with SlSGS3, the tomato homolog of the Arabidopsis SGS3 protein (AtSGS3), which is known to be involved in the RNA-silencing pathway. SlSGS3 genetically complemented an AtSGS3 mutation and restored RNA silencing, indicating that SlSGS3 is indeed a functional homolog of AtSGS3. A point mutant of V2 that is unable to bind SlSGS3 also lost its ability to suppress RNA silencing, suggesting a correlation between the V2–SlSGS3 interaction in planta and the suppressor activity of V2.

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Section: P Lants Have Evolved An Innate Immune Response To Invadingmentioning

confidence: 99%

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Glick

Zrachya

Levy

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

272

202

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“…activity of p20 was first suggested in the study that identified the p20 homolog of Beet yellows closterovirus as a silencing suppressor (10,35). p20 suppression shares features with silencing suppression by CMV 2b because both are potent suppressors of intercellular silencing but incomplete in suppressing intracellular silencing (21).…”

Section: Discussionmentioning

confidence: 98%

Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome

Folimonov

Shintaku

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

351

248

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Viral infection in both plant and invertebrate hosts requires a virus-encoded function to block the RNA silencing antiviral defense. Here, we report the identification and characterization of three distinct suppressors of RNA silencing encoded by the Ϸ20-kb plus-strand RNA genome of citrus tristeza virus (CTV). When introduced by genetic crosses into plants carrying a silencing transgene, both p20 and p23, but not coat protein (CP), restored expression of the transgene. Although none of the CTV proteins prevented DNA methylation of the transgene, export of the silencing signal (capable of mediating intercellular silencing spread) was detected only from the F1 plants expressing p23 and not from the CP-or p20-expressing F1 plants, demonstrating suppression of intercellular silencing by CP and p20 but not by p23. Thus, intracellular and intercellular silencing are each targeted by a CTV protein, whereas the third, p20, inhibits silencing at both levels. Notably, CP suppresses intercellular silencing without interfering with intracellular silencing. The novel property of CP suggests a mechanism distinct to p20 and all of the other viral suppressors known to interfere with intercellular silencing and that this class of viral suppressors may not be consistently identified by Agrobacterium coinfiltration because it also induces RNA silencing against the infiltrated suppressor transgene. Our analyses reveal a sophisticated viral counter-defense strategy that targets the silencing antiviral pathway at multiple steps and may be essential for protecting CTV with such a large RNA genome from antiviral silencing in the perennial tree host.RNA interference ͉ citrus tristeza virus ͉ virus synergy ͉ antiviral immunity T he discoveries of viral suppressors of RNA silencing play an important role in establishing RNA silencing as a natural antiviral response in both plant and invertebrate hosts (1). Many plant viral proteins have been identified as suppressors of RNA silencing since the initial reports in late 1998 (2-4). RNA silencing suppressors from different virus taxons are structurally diverse. However, they are typically required for long-distance virus spread and influence virulence and accumulation levels in infected plants (5-7). Progress is being made toward understanding the molecular mechanisms involved in viral suppression of RNA silencing (8-11). In RNA silencing assays, plant viral suppressors differ by their ability to suppress intracellular and͞or intercellular silencing (12-16). Intercellular silencing is mediated by the non-cell-autonomous silencing signal that can spread to destroy homologous RNAs in neighboring or distant tissues that do not contain the initial trigger, such as a silencing transgene or a replicating virus (17-19). The differential silencing suppression is best illustrated by the potyviral helper component proteinase (HC-Pro) and the cucumber mosaic virus (CMV) 2b protein. When introduced by genetic crosses into the Nicotiana tabacum line 6b5 carrying a silencing ␤-glucuronidase (GUS) transgen...

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“…To determine whether the inhibition of recombinant TYMV replication would be relieved by viral RNA silencing suppressors, we introduced the TY-GUS construct into N. benthamiana along with potyviral HC-Pro from Tobacco etch virus (18), closteroviral p21 from Beet yellows virus (19), or tombusviral p19 from Tomato bushy stunt virus (20). Using this strategy, the replication of the recombinant TYMV recovered to the wildtype level when one of the viral RNAi suppressors was co-expressed ( Fig.…”

Section: Replication Of the Recombinant Tymv Containing A Heterologoumentioning

confidence: 99%

Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA

Shin

Kim²,

Cho

2008

BMB Reports

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Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus that infects mainly Cruciferae plants. In this study, the TYMV genome was modified by inserting an extra subgenomic RNA promoter and a multiple cloning site. This modified TYMV was introduced into Nicotiana benthamiana using a Agrobacterium-mediated T-DNA transfer system (agroinfiltration). When a gene encoding β-glucuronidase or green fluorescent protein was expressed using this modified TYMV as a vector, replication of the recombinant viruses, especially the virus containing β-glucuronidase gene, was severely inhibited.

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Suppressor of RNA silencing encoded by Beet yellows virus

Cited by 132 publications

References 31 publications

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein

Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome

Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA

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