Suppressor cell regulation of cell-mediated immune responses in renal infection in vitro modulation of suppressor cell activity.

Abstract

Hospital, Aucklanid, News Zeala 11(d A B S T R A C T Infection-induced anergy is a fre(luent complication of bacterial, viral, and parasitic infection. A marked suppression of the thymus-derived (T) lymphocyte response to concanavalini A has been demonstrated in vitro during renal infection and the mechaniismiis by which suppressioni occurs have been investigated. In particular we have considered the possibility that suppressioni might result from the inhibitory effect of prostaglandins, secreted by activated macrophages with immunoregulatory potential. The experiments have shown that the T-lymphocyte effector status in experimentally-induced renal infection is determined by two suppressor cells, one infectioni-induced and the other naturally occurring. The inability to respond to mitogenic stimulation was reversible and restoration of immnune responsiveness to splenic lymphocytes from infected animals could be achieved in two stepwise manipulations; differential centrifugation removed the infection-induced suppressor cells, and the suppressor activity of the naturally occurring suppressor cells could then be inhibited by indomethacin. Thus the two suppressor cells were distinguishable on the basis of their physical characteristics and their response to indomethacin. The dominant factor determininig the immune responsiveness of splenic lymphocytes from the pyelonephritic animals was, however, the infection-induced suppressor cell. This cell has been characterized as a sedimentable cell (30 g) with suppressor activity demonistrable in coculture experiments. Plastic-adherent cells from the sedimentable fraction of pyelonephritic animals' splenic cells were shown to have suppressor activity that was not inhibited by indomethacin. The infection-induced and naturally occurring suppressor cells can be viewed as prototypes for the equivalent cells in man and may be useful models for studying the

Urinary System

“…This primary reflux, possibly in combination with a secondary effect in the intrarenallymphatic system, may play an important role (Corriere and Murphy 1967). It seems probable that cell-mediated mechanisms are involved in the pathogenesis of certain tubulointerstitial diseases, in particular pyelonephritis, in which the dominant factor determining the immune responsiveness is the infection-induced suppressor cell (Miller and Marshall 1980). It seems probable that cell-mediated mechanisms are involved in the pathogenesis of certain tubulointerstitial diseases, in particular pyelonephritis, in which the dominant factor determining the immune responsiveness is the infection-induced suppressor cell (Miller and Marshall 1980).…”