Suppressive Effects of Lactoferrin on Bleomycin-dependent DNA Damage by the Iron Ion and Ascorbate

Lactoferrin and Cancer Disease Prevention

“…Finally, despite the evidence that LF possesses chemopreventive activity, its anti-cancer activity against established tumours, ability to potentiate chemotherapy as described with other immunotherapeutics, and the immune mechanisms by which its anti-tumour activity is mediated, is still largely unknown. Levay and Viljoen, 1995;Steijns and Van Hooijdonk, 2000;Babina et al, 2006;Shimazaki, 2000 Pierce et al, 1991;Baker, 1994 Molecular weight 77 ± 1.5 76.8 ± 1.6 Querinjean et al, 1971;Spik et al, 1994;Castellino et al, 1970 Isoelectric point 8.5-9.0 8.4-9.0 Spik et al, 1994;Lampreave et al, 1990;Zakharova et al, 2000 Kawakami et al, 1993;Mikogami et al, 1995;Levay and Viljoen, 1995;Ward et al, 2005 Antioxidant Iron scavenger LF has the ability to bind to cell membranes enhancing its ability to prevent iron-mediated lipid peroxidation Matsue et al, 1994;Konishi et al, 2006;Larkins, 2005 Antimicrobial Growth inhibition by iron scavenging or membrane disintegration LF has a powerful iron-binding capacity and shows a strong interaction with other molecules and cell surface Nemet and Simonovits, 1985 ;Yamauchi et al, 1993 ;Qiu et al, 1998 ;Weinberg, 2007 ;Santagati et al, 2005;Lönnerdal, 2003 ;Lee et al, 2005 Antiviral Prevention of virus attachment, inhibition of virus replication, blocking of cell-virus interactions Yi et al, 1997 ;Siciliano et al, 1999 ;Andersen et al, 2001 ;Ikeda et al, 2000 ;Giansanti et al, 2002 ;Longhi et al, 2006;Mistry et al, 2007;…”

Occurrence, structure, biochemical properties and technological characteristics of lactoferrin

“…Using site-directed mutagenesis Ward et al (1996) purified mutant C-and N-lobe human lactoferrins from Aspergillus awamori; in these lactoferrins the two tyrosine residues involved in the iron binding were changed for Kawakami et al 1988Kawakami et al , 1993Mikogami et al 1995 Antioxidant Iron scavenger Matsue et al 1994 Growth inhibition by iron scavenging or membrane desintegration Reiter, 1985;Naidu & Arnold, 1997 Antiviral Prevention of virus attachment Harmsen et al 1995;Marchetti et al 1996;Yi et al 1997 Anti-inflammatory, immune modulating LPS binding, stimulation of NK cells, reduction of pro-inflammatory cytokines, T-cell maturation Zimecki et al 1991;Cohen et al 1992;Mattsby-Baltzer et al 1996;Shimizu et al 1996 Anti-cancer Unknown Bezault et al 1994;Sekine et al 1997;Yoo et al 1997 alanine either in the C-lobe or the N-lobe or both. Their studies suggest that the C-lobe contributes more to iron stabilization than the N-lobe.…”

“…increase shelf life. The mechanism by which lactoferrin exerts this antioxidant effect is likely the control of the iron or copper catalysed Fenton reaction, which generates the potent hydroxyl free radical from peroxides (Matsue et al 1994(Matsue et al , 1995. This mechanism is different from the radical quenching effects exerted by antioxidant vitamins like tocopherols.…”

Structural characterization and functional evaluation of lactoferrin–polyphenol conjugates formed by free-radical graft copolymerization

“…12,13 The antioxidant properties of LF have been demonstrated in various biochemical environments. 14,15 It was suggested that a combination of LF with polyphenols may have synergistic effects on inhibiting cancer development. 16 Phenolic compounds can covalently react with proteins via enzymatic or non-enzymatic oxidation.…”