Suppressive effects ofSchizandra chinensisBaillon water extract on allergy-related cytokine generation and degranulation in IgE-antigen complex-stimulated RBL-2H3 cells

Abstract

Schizandra chinensis Baillon is a traditional folk medicine plant that is used to treat and prevent several inflammatory diseases and cancer in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. This study was designed to investigate mechanisms of anti-allergic activity of a Schizandra chinensis Baillon water extract (SCWE) in immunoglobulin E (IgE)-antigen complex-stimulated RBL2H3 cells and to assess whether gastric and intestinal digestion affects the anti-allergic properties of SCWE. Oxidative stress is an important consequence of the allergic inflammatory response. The antioxidant activities of SCWE increased in a concentration-dependent manner. RBL-2H3 cells were sensitized with monoclonal anti-dinitrophenol (DNP) specific IgE, treated with SCWE, and challenged with the antigen DNP-human serum albumin. SCWE inhibited β-hexosaminidase release and expression of interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha (TNF-α) mRNA and protein in IgE-antigen complex-stimulated RBL2H3 cells. We found that digested SCWE fully maintained its antioxidant activity and anti-allergic activity against the IgE-antigen complex-induced activation of RBL-2H3 cells. SCWE may be useful for preventing allergic diseases, such as asthma. Thus, SCWE could be used as a natural functional ingredient for allergic diseases in the food and/or pharmaceutical industries.

Formoterol synergy with des-ciclesonide inhibits IL-4 expression in IgE/antigen-induced mast cells by inhibiting JNK activation

“…Initially, we confirmed previously reported observations (Chung et al, 2012) in which the increase in IL-4 and IL-13 mRNA and protein expression was induced by the IgE-antigen complex in RBL-2H3 cells. IL-4 and IL-13, from mast cell products, regulate both IgE synthesis and result in the development of eosinophilic inflammation (Bradding et al, 2006).…”

“…2A and B) and IL-13 ( Fig. 2C and D) in a concentration-dependent manner in IgE-sensitized RBL-2H3 cells, which is consistent with previous studies (Chung et al, 2012).…”

Hispidulin Inhibits Mast Cell-Mediated Allergic Inflammation through Down-Regulation of Histamine Release and Inflammatory Cytokines

“…Anti-DNP IgE-primed and DNP-HSA-stimulated mast cells induce allergic responses (induction of histamine and β-hexosaminidase release and Th2 cytokines expression). However, these responses do not occur in the absence of sensitization with anti-DNP IgE or in the absence of stimulation with DNP-HSA [24,25,26,27]. Therefore, we performed our experiments in the condition of their presence with anti-DNP IgE sensitization and DNP-HSA stimulation.…”

Resveratrol Inhibits IgE-Mediated Basophilic Mast Cell Degranulation and Passive Cutaneous Anaphylaxis in Mice

“…Rat basophilic leukemic RBL-2H3 mast cells were cultured in EMEM containing 10% heat-inactivated FBS, 2 mmol/L glutamine, 100 mg/L streptomycin, and 100,000 U/L penicillin at 37°C in a humidified atmosphere of 5% CO 2 in air. RBL-2H3 cells were sensitized with 0.5 mg/L anti-DNP IgE overnight to induce IgE-mediated allergic responses (18). Sensitized cells were washed twice in piperazine-N,NÕ-bis (2-ethanesulfonic acid) (PIPES) buffer containing 0.4 mmol/L MgCl 2 , 40 mmol/L NaOH, 119 mmol/L NaCl, 1 mmol/L CaCl 2 , 5 mmol/L KCl, 25 mmol/L PIPES, 5.6 mmol/L glucose, and 1% BSA (pH 7.4) and pretreated with 1-25 mmol/L resveratrol for 2 h. Subsequently, cells were exposed to 200 mg/L DNP-HSA for the various times indicated.…”