Suppressive Effects of Fucoxanthin on Degranulation in IgE-antigen complex-stimulated RBL-2H3 Cells

Abstract

The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of β-hexosaminidase and suppressed not only transcriptional activation of NF-κB, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.

Inhibitory Effect of Alpiniae officinarum Rhizoma Extract on Degranulation in RBL-2H3 Cells

“…IL-4 causes IgE mediated allergic reaction (Hirasawa et al, 2000). In the present study, we (Chang and Karin, 2001;Kim and Choi, 2010;Namkoong et al, 2012). In this study, we investigated the effect of AOR extract on MAPK signal pathway in RBL-2H3 cells.…”

Comparison of the anticancer effect of microalgal oils and microalgal oil-loaded electrosprayed nanoparticles against PC-3, SHSY-5Y and AGS cell lines

“…In another study, cytotoxic effect of extracts from Eisenia bicyclis algae was tested on the RBL-2H3 rat mast cell line, HaCaT human keratinocyte cell line and NIH3T3 mouse fibroblast cell line. As a result of the study, it was observed that the extracts had no toxic effect on HaCaT and NIH3T3 cells and it did not create toxic effect on RBL-2H3 cells to a certain concentration [20]. Yan et al investigated the production of cellulose/microalgae films, and it was reported that, L929 cells were able to adhere on the films which were created by using Nannochloropsis and cellulose, and the cells cultured on the composite films proliferated better than those on the pure cellulose film [21].…”

Fucoxanthin: A Promising Phytochemical on Diverse Pharmacological Targets

“…In IgE/DNP-BSA-induced RBL-2H3 cells, they found that FX inhibited the release of β-hexosaminidase, transcriptional activation of nuclear factor kappa B (NF-κB), and phosphorylation of c-Jun N-terminal kinases (JNK) and extracellular regulated kinase (ERK). So, FX may aid in the prevention of allergic disorders including asthma and atopic dermatitis ( Namkoong et al, 2012 ).…”

“…Generally, microalgae have a higher concentration of FX than macroalgae (Kim S. M. et al, 2012). Chlorophyceae (Green algae), Rhodophyceae (Red algae), and Phaeophyceae (Brown algae) are the three major classes of marine algae (Khan et al, 2010).…”