Suppressive Effect of Monocytes in Vitro in Patients with Carcinoma of the Uterine Cervix

Bjercke, Sverre; Onsrud, Mathias; Gaudernack, Gustav

doi:10.3109/00016348609158399

Cited by 4 publications

(5 citation statements)

References 22 publications

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“…A stage-correlated increase in circulating monocytes was demonstrated. This confirms our earlier reported findings where also a monocyte/macrophage-induced suppression of lymphoproliferative responses was demonstrated (8). Our findings are also in accord with those of Check et al (9).…”

Section: Discussionsupporting

confidence: 94%

“…Aberrations in numbers and responses of immunoactive cells have been described in cervical cancer (4,5,8). Most of these studies show an increasing impairment of immune function with increasing stage ( 5 , 8 ) .…”

Section: Discussionmentioning

confidence: 99%

“…Suppression of T-lymphocyte responses can also be exerted by cells of the monocyte/macrophage scries (6). This type of suppression has been described in various types of malignancy (7) including advanced cervical carcinoma (8). The aberrations in the immune system could be real determinants for the cancer development.…”

mentioning

confidence: 99%

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Lymphoid cell distribution as prognostic factor in carcinoma of the uterine cervix

Onsrud¹,

Grahm

Gaudernack³

et al. 1992

Acta Obstet Gynecol Scand

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Pretreatment assessment of blood lymphoid cells was performed in 44 patients with carcinoma of the cervix and in 19 healthy controls. White blood cells were determined by routine differential counting, and T-lymphocyte subsets and monocytes were quantitated using monoclonal antibodies. Increase in monocyte numbers, as determined by the 1D5 antibody, was seen in the cancer patients, especially in the group with advanced disease. No change in T-lymphocyte subpopulations could be found. During the 5-year follow-up period, 17 patients had a recurrence or died of cancer. The best prognostic information was obtained from conventional clinical parameters, e.g. stage, tumor size and lymph node status. Increased numbers of granulocytes and monocytes were found in advanced stage disease but had no independent prognostic influence. In pelvic lymph node biopsies taken from patients undergoing Wertheim-Meigs operation the T-helper/T-suppressor ratio was higher and the monocyte number lower than in peripheral blood. No correlation could be detected between node cell distribution and the prognosis. It is concluded that immunological testing, as performed in this study, elicits very little new prognostic information.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Lymphoid cell distribution as prognostic factor in carcinoma of the uterine cervix

Onsrud¹,

Grahm

Gaudernack³

et al. 1992

Acta Obstet Gynecol Scand

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“…A possible explanation for this impaired function of the lymphocytes and monocytes could be the presence of circulating immunosuppressive factors or an induction of suppressor cell populations as already postulated by other authors (Zembala et al, 1977;Bjercke et al, 1986).…”

Section: Discussionmentioning

confidence: 93%

Impaired cytokine production in whole blood cell cultures of patients with gynaecological carcinomas in different clinical stages

Elsässer‐Beile

Kleist²,

Sauther³

et al. 1993

Br J Cancer

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Summary The production of the cytokines IFN-gamma, IL-1-alpha, IL-2 and TNF-alpha was investigated in mitogen-stimulated, whole blood cell culturs from 239 untreated patients with primary gynaecological carcinomas (breast, cervix, ovary, endometrium), and 191 healthy female controls.The cytokines were measured in the 4-day post-inductional supernatants by a sensitive enzymoimmunological assay. In the blood cell cultures of all four groups of cancer patients, significantly lower values of IFN-gamma (P < 0.001), IL-2 (P < 0.01) and IL-1 alpha (P < 0.01) were found as compared to the controls, although lymphocyte and monocyte counts were almost identical.Grouping the tumour patients into different clinical stages we could show in the four groups of carcinomas a gradual depression of the cytokine production according to growing tumour burden.The host's response to malignant tumours is usually considered to be primarily a function of the cellular immune system and various tests have been established to measure the integrity of this system in cancer patients, such as hypersensitivity skin tests (Bolton et al., 1975;Evans et al., 1977), or determination of mitogen-induced lymphocyte proliferation in vitro (Dean et al., 1977;Zembala et al., 1977;Fritze & Dystant, 1984).As the cell-mediated immune response is controlled by a series of soluble cytokines, in recent years the measurement of these substances has also been used to determine the cellular immunological potency of tumour patients (Rey et al., 1983;Santos et al., 1985;Elsasser-Beile et al., 1991a Blood samples Ten ml of heparinised blood was taken from each patient and control person between 9 and 11 a.m. The samples, kept at room temperature, were used within 2 h. A 0.5 ml aliquot was removed for total and differential leukocyte counts.Whole blood cell culture (WBCC) Cultures were performed as previously described with a system for which optimal conditions and kinetics of cytokine production were established (Elsasser-Beile et al., 1991b).In brief, heparinised venous blood was diluted 1/10 with RPMI 1640 (Seromed, Berlin FRG), which was supplemented with 50 U ml-' Penicillin (Seromed) and 50 Sg ml-' Streptomycin (Seromed) and distributed in 0.5 ml aliquots in 12 mm polystyrol tubes. For stimulation, 10 ig ml-' phytohaemagglutinin (PHA Wellcome, Burgwedel, FRG) and 0.5-5 g.g ml-' Pokeweed mitogen (PWM, Sigma, Deisenhofen, FRG) were added.Incubation of the cell cultures was performed at 37°C in a humidified atmosphere of 5% CO2. After 4 days of culture without changes of medium 320 ftl of supernatant was removed from each tube to be assayed for cytokine levels.Determination of cytokines Enzyme-linked immunoassays (ELISA) were applied for qualitative and quantitative determinations of cytokines.These tests are based on the sandwich principle and are performed in one step. Since a correlation was found between assay performance of the naturally occurring cytokines and their recombinant counterparts, the latter were used as standards.Interferon-gamma-ELISA IFN-gamma ...

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“…Immunosuppression caused by monocytes has been described in several types of cancer. In a recent study on patients with cervical carcinoma, we observed a potentiation of T cell responses to microbial antigens after removal of monocytes by adherence on gelatinhibronectin coated surfaces (3). This type of immunosuppression is presumably of greater importance in patients presenting high monocyte/T cell ratios.…”

Section: Discussionmentioning

confidence: 96%

Early Stage Carcinoma of the Uterine Cervix Effects of Intracavitary Radium Treatment on Lymphoid Cells in Blood and Pelvic Lymph Nodes

Onsrud

Grahm

Gaudernack

1986

Acta Radiologica: Oncology

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Suppressive Effect of Monocytes in Vitro in Patients with Carcinoma of the Uterine Cervix

Cited by 4 publications

References 22 publications

Lymphoid cell distribution as prognostic factor in carcinoma of the uterine cervix

Lymphoid cell distribution as prognostic factor in carcinoma of the uterine cervix

Impaired cytokine production in whole blood cell cultures of patients with gynaecological carcinomas in different clinical stages

Early Stage Carcinoma of the Uterine Cervix Effects of Intracavitary Radium Treatment on Lymphoid Cells in Blood and Pelvic Lymph Nodes

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