Suppressive effect of microRNA-126 on oral squamous cell carcinoma in vitro

Yang, Xiaolun; Wu, Hanjiang; Ling, Tianyou

doi:10.3892/mmr.2014.2171

Cited by 42 publications

(36 citation statements)

References 20 publications

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“…Many studies have highlighted the strong downregulation of miRNA-126 in several cancer types such as pancreatic [66], lung, and kidney ones [67, 68]. miRNA-126 is often referred to as a tumor suppressor [69]. Angiogenesis has a pivotal role in chronic inflammation, tumor progression, and metastasis [70, 71].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression in Malignant Pleural Mesothelioma and Asbestosis: A Pilot Study

et al. 2017

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Background The identification of diagnostic/prognostic biomarkers for asbestos-related diseases is relevant for early diagnosis and patient survival and may contribute to understanding the molecular mechanisms underlying the disease development and progression. Aims To identify a pattern of miRNAs as possible diagnostic biomarkers for patients with malignant pleural mesothelioma (MPM) and asbestosis (ASB) and as prognostic biomarkers for MPM patients. Methods miRNA-16, miRNA-17, miRNA-126, and miRNA-486 were quantified in plasma and formalin-fixed paraffin-embedded samples to evaluate their diagnostic and prognostic roles compared to patients with other noncancerous pulmonary diseases (controls). Results. The expression of all the miRNAs was significantly lower in patients with MPM and ASB than that in controls. miRNA-16, miRNA-17, and miRNA-486 in plasma and tissue of MPM patients were significantly correlated. Furthermore, the expression of miRNA-16 in plasma and tissue, and miRNA-486 only in tissue, was positively related with cumulative survival in MPM patients. Conclusions All the miRNA levels were decreased in patients with MPM or ASB, supporting the role of circulating miRNAs as a potential tool for diseases associated with exposure to asbestos fibers. miRNA-16 was directly related to MPM patient prognosis, suggesting its possible use as a prognostic marker in MPM patients.

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Section: Discussionmentioning

confidence: 99%

MicroRNA Expression in Malignant Pleural Mesothelioma and Asbestosis: A Pilot Study

et al. 2017

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“…In the head and neck, specific patterns of miRNA expression have been detected in cell lines and tumoral tissues from the hypopharynx, oral cavity, larynx, nasopharynx and salivary glands (23). Specific miRNAs that have been implicated in oral SCC cell lines and tumoral tissues include miR-211 (22), miR-184 (24), miR-137, miR-193a (25), miR-133a, miR-133b (26), miR-98 (27), Homo sapiens (hsa)-miR-15a (28), miR-126 (29) and miR-155 (30). Certain patterns of miRNA expression can also serve as prognostic markers.…”

Section: Introductionmentioning

confidence: 99%

Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

et al. 2016

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Oral cavity squamous cell carcinoma may be more aggressive at presentation and recurrence in young patients compared with older patients. Dysregulation of microRNAs (miRNAs or miRs) has been associated with the development and prognosis of oral cavity cancer. The present study investigated miRNA expression in carcinoma of the oral tongue in young patients. miRNA expression profiles were evaluated in formalin-fixed, paraffin-embedded samples of tumor and normal mucosa from 12 patients aged <30 years old with squamous cell carcinoma of the tongue. The levels of let-7f-5p, miR-30b-5p and let-7e-5p were upregulated in tumors (P<0.05). The expression of let-7f-5p was upregulated in non-aggressive tumors, while the expression of let-7e-5p was upregulated in aggressive tumors, compared with the corresponding normal tissue. Aggressive tumors had higher levels of let-7c, miR-130a-3p, miR-361-5p, miR-99a-5p, miR-29c-3p and let-7d-5p than non-aggressive tumors (P<0.05). The findings remained significant for let-7c upon false-discovery rate correction. An excellent correlation was noticed on validation of NanoString counts by quantitative polymerase chain reaction. The comparison with published findings in adults demonstrated a unique miRNA signature in young patients with aggressive disease. Aggressive oral cavity cancer in patients <30 years old is associated with a distinctive expression pattern of the let-7 family. Larger studies including direct comparison with older patients are warranted.

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“…Many studies have suggested that miR-126-3p plays a part as either an anti-oncogene or an oncogene in different cancers [16, 17]. miR-126-3p also acts as a tumor suppressor via the downregulation of intron 7 of epidermal growth factor-like domain 7 (EGFL7) and it may be a promising candidate for therapeutic strategies in oral squamous cell carcinoma [18]. Some studies have demonstrated that miR-126-3p stimulates angiogenesis by targeting PIK3R2 proteins, which are negative regulators of the vascular endothelial growth factor (VEGF) signal pathway [19].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-126-3p suppresses cell proliferation by targeting PIK3R2 in Kaposi's sarcoma cells

Zhao

Kang

et al. 2016

Oncotarget

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Kaposi's sarcoma is a highly vascular tumor of lymphatic endothelial origin. Many deregulated miRNAs, including miR-126-3p, have been identified in Kaposi's sarcoma tissues. miR-126-3p is the most highly endothelial-specific miRNA that regulates vascular integrity and angiogenesis. In this study, we aimed to determine the effect of miR-126-3p on Kaposi's sarcoma cells through transfection of a miRNA mimic and inhibitor. Moreover, we searched the target gene (PIK3R2) of miR-126-3p using bioinformatics software and further verified PIK3R2 using luciferase reporter assays, Real-time quantitative PCR (qRT-PCR) and western blot. The results demonstrated that miR-126-3p inhibited cell proliferation, arrested cell cycle progression, induced cell apoptosis, and inhibited cell invasion of SLK cells. The bioinformatics analysis and luciferase reporter assay revealed that PIK3R2 mRNA is a direct target of miR-126-3p. Moreover, the level of expression of the PIK3R2 gene was downregulated in SLK cells transfected with miR-126-3p siRNAs. Therefore, our data demonstrated that miR-126-3p is a tumor suppressor miRNA that acts by targeting PIK3R2 in Kaposi's sarcoma cells. These findings contribute to our understanding of the molecular mechanisms underlying Kaposi's sarcoma.

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Suppressive effect of microRNA-126 on oral squamous cell carcinoma in vitro

Cited by 42 publications

References 20 publications

MicroRNA Expression in Malignant Pleural Mesothelioma and Asbestosis: A Pilot Study

MicroRNA Expression in Malignant Pleural Mesothelioma and Asbestosis: A Pilot Study

Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

MicroRNA-126-3p suppresses cell proliferation by targeting PIK3R2 in Kaposi's sarcoma cells

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