Suppressive effect of low dose total body irradiation on lung metastasis: dose dependency and effective period

Hosoi, Yoshio; Sakamoto, Kiyohiko

doi:10.1016/0167-8140(93)90101-d

Cited by 75 publications

(50 citation statements)

References 8 publications

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“…The data in Table 4 show that, when metastatic-melanoma-bearing newborn rats are whole-body irradiated with a dose of 20 cGy immediately after the cell injection, a suppression of the metastatic potential of the tumour occurs. Consistently with our results, it was observed that the number of artificial lung metastases of a murine squamous cell carcinoma is reduced about twofold when the mice are wholebody irradiated, immediately after the tumour cell injection, with a British Journal of Cancer (1997) 75(5), [639][640][641][642][643][644][645][646][647][648][649] dose of 20 cGy; although the effect was thought to result from a radiation-induced stimulation of the immune system (Hosoi and Sakamoto, 1993). As the results on the hyper-radiosensitivity of the highly metastatic cells were obtained using a clonogenic assay, with the cells plated at low density and irradiated 24 h later, this phenomenon would be relevant in a clinical situation under the same experimental conditions.…”

Section: The Survival Curves Of the Seven Clones And Variants Differ mentioning

confidence: 99%

The gangliosides as a possible molecular coupling factor between the proportion of radiosensitive cells in vitro and the metastatic potential in vivo within a human melanoma cell line

Thomas

Buronfosse²,

Portoukalian³

et al. 1997

Br J Cancer

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Summary With an experimental model of spontaneous lung metastases in immunosuppressed newborn rats, seven clones and variants with different metastatic potential and gangliosides expression were derived from a single parental human melanoma cell line M4Be. The cellular radiosensitivity of M4Be and its seven sublines was estimated using an in vitro colony assay. The total amount of gangliosides in M4Be and its seven sublines was determined by cell extraction and thin-layer chromatography, while the expression of GD3 gangliosides was estimated by flow cytometry with a monoclonal antibody. The radiation-cell survival curves of most clones and variants derived from M4Be showed a zero dose extrapolation clearly lower than 100%, suggesting that two populations of cells of very different radiosensitivity coexist within each of these clones and variants. Although the proportion of radiosensitive cells could be estimated from the shape of the survival curve, its radiosensitivity is too high to be properly evaluated by the colony assay. The eight survival curves differ essentially in the proportion of radiosensitive cells -which varied from 0% to 40% among M4Be and its seven sublines -whereas the cellular radiosensitivity of the radioresistant population was similar among them. The metastatic potential in vivo of M4Be and its seven sublines was not significantly related to the cellular radiosensitivity of their corresponding radioresistant population, but significantly increased with the fraction of radiosensitive cells. This relationship is valid only when the highly metastatic cells are cultured for no more than five passages in vitro as the fraction of radiosensitive cells is rapidly lost during subcultures. The relationship remains valid in vivo as metastatic melanoma-bearing newborn rats whole body irradiated with 20 cGy show no lung metastasis compared with controls. The radiosensitive cell fraction is inversely correlated with both the total ganglioside content (r= 0.84, P < 0.02) and the number of cells positively labelled with the monoclonal antibody directed to GD3 (r= 0.92, P < 0.001). The incubation of a radiosensitive clone with the exogenous bovine brain ganglioside GM1 significantly increases the proportion of radioresistant cells and suppresses its metastatic potential, while the inhibition of the endogenous gangliosides synthesis in the radioresistant cell line M4Be increases the proportion of radiosensitive cells. This study provides a possible explanation for the correlation between the metastatic potential and the proportion of radiosensitive cells within the seven sublines derived from a single parental human melanoma cell line.

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Section: The Survival Curves Of the Seven Clones And Variants Differ mentioning

confidence: 99%

The gangliosides as a possible molecular coupling factor between the proportion of radiosensitive cells in vitro and the metastatic potential in vivo within a human melanoma cell line

Thomas

Buronfosse²,

Portoukalian³

et al. 1997

Br J Cancer

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“…The effect of TBI on metastasis is described in detail elsewhere (Hosoi and Sakamoto, 1993) and is summarized only briefly here.…”

Section: Effect Of Tbi On Metastasismentioning

confidence: 99%

“…Relative values of lung colonies in mice versus exposed dose of X-ray given 9 h before tumor-cell injection into tail vein (Hosoi and Sakamoto, 1993); * p ≤ 0.05. of tumor cells though a blood vessel may not reflect the effects of TBI on spontaneous metastasis because metastases from human tumors originate through lymphatic flow. Therefore, the suppressive effect of low dose of TBI on spontaneous lung metastasis in WHT/Ht mice was investigated.…”

Section: Figure 10mentioning

confidence: 99%

“…In these experiments, mice were subjected to TBI of various doses 12 days after tumor-cell injection into their groins. Lung colonies were counted 20 days FIGURE 11 Effect of 15 cGy of TBI on artificial lung-colony formation: TBI given at various times before or after tumor-cell injection into tail vein (Hosoi and Sakamoto, 1993); * p ≤ 0.05, * * p ≤ 0.01.…”

Section: Figure 10mentioning

confidence: 99%

“…Effect of TBI dose on spontaneous lung metastasis: TBI given 12 days after tumor-cell transplantation into groin (Hosoi and Sakamoto, 1993); * p ≤ 0.05.…”

Section: Figure 12mentioning

confidence: 99%

See 2 more Smart Citations

Radiobiological Basis for Cancer Therapy by Total or Half-Body Irradiation

Sakamoto¹

2004

Nonlinearity in Biology, Toxicology, Medicine

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Effect of combination treatment of 15 cGy total body irradiation and OK-432 on spontaneous lung metastasis and mitogenic response of splenocytes in mice

Hosoi

Ishii

Yamada

et al. 1997

Radiat. Oncol. Investig.

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We investigated whether the combination treatment of 15 cGy total body irradiation (TBI) and a streptococcal preparation, OK‐432, synergistically suppresses spontaneous lung metastasis and augments phytohemagglutinin (PHA) and concanavalin A (Con A) responses of splenocytes in WHT/Ht mice. TBI with 15 cGy was carried out 20 days after subcutaneous injection of squamous cell carcinoma cells into a hind leg. Lung colony number was counted 40 days after tumor injection. For PHA and Con A responses, mice were killed 4 hr after 15 cGy TBI. The combination treatment of 15 cGy TBI and OK‐432 was most effective when OK‐432 was administered 2 days before 15 cGy TBI. The combination treatment decreased the lung colony number to 29.9% of the control number. OK‐432 slightly increased the PHA and Con A responses, and 15 cGy TBI did not increase them. However, when these two were combined, the PHA and Con A responses were significantly increased to 393% and 278% of the control levels, respectively. It was suggested that TBI and OK‐432 acted synergistically in suppressing the lung metastasis and mitogenic response of splenocytes. Radiat. Oncol. Invest. 5:283–288, 1997. © 1997 Wiley‐Liss, Inc.

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Suppressive effect of low dose total body irradiation on lung metastasis: dose dependency and effective period

Cited by 75 publications

References 8 publications

The gangliosides as a possible molecular coupling factor between the proportion of radiosensitive cells in vitro and the metastatic potential in vivo within a human melanoma cell line

The gangliosides as a possible molecular coupling factor between the proportion of radiosensitive cells in vitro and the metastatic potential in vivo within a human melanoma cell line

Radiobiological Basis for Cancer Therapy by Total or Half-Body Irradiation

Effect of combination treatment of 15 cGy total body irradiation and OK-432 on spontaneous lung metastasis and mitogenic response of splenocytes in mice

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