Suppressive effect of dexamethasone on murine Th9 cell-mediated nasal eosinophilic inflammation

Koyama, Teidai; Miura, Kento; Yamasaki, Norimasa; Ogata, Sawako; Ito, Daiki; Saeki, Mayumi; Hiroi, Takachika; Mori, Akio; Kaminuma, Osamu

doi:10.5415/apallergy.2021.11.e25

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…[1][2][3] Inflammatory responses in the nasal mucosa are typical features of AR and are frequently accompanied by the infiltration of immune cells, including T cells, eosinophils, and basophils. 4,5 Previous studies have confirmed that the microbiome directly affects inflammatory responses in allergic diseases such as AR and asthma. [6][7][8] Therefore, the study of the composition of the microbiota is of great importance for understanding the pathogenesis of AR.…”

Section: Introductionmentioning

confidence: 96%

Microbiome profiling of nasal extracellular vesicles in patients with allergic rhinitis

Chiang¹,

Yang²,

Zhuo³

et al. 2022

World Allergy Organization Journal

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Section: Introductionmentioning

confidence: 96%

Microbiome profiling of nasal extracellular vesicles in patients with allergic rhinitis

Chiang¹,

Yang²,

Zhuo³

et al. 2022

World Allergy Organization Journal

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“…At the start of culturing, pre-washed Dynabeads Mouse T-Activator CD3/CD28 (2 μL/10 5 cells, Thermo Fisher Scientific) and recombinant IL-2 (10 U/mL, PeproTech, Inc., Cranbury, NJ, USA) were added. To promote differentiation into each subset, cells were cultured for 7 to 10 days in the presence of appropriate cytokines and anti-cytokine antibodies as follows[7][8][9][10][11][12]. Th1: 10 U/mL IL-PeproTech) and 10 µg/mL anti-IL-4 monoclonal antibody (mAb) (BD Biosciences, San Jose, CA, USA), Th2: 10 U/mL IL-4 (PeproTech) and 10 μg/mL anti-IFN-γ mAb (R4-6A2, eBioscience, San Diego, CA, USA), Th9: 10 U/mL IL-4, 5 ng/mL TGF-β (BioLegend, San Diego, CA, USA), and 10 μg/mL anti-IFN-γ mAb, Th17: 10 ng/mL IL-1β (Peprotech), 20 ng/mL IL-6 (Peprotech), 1 ng/mL TGF-β, 10 ng/mL IL-23 (R & D Systems, Minneapolis, MN, USA), 40 μg/mL anti-IL-4 mAb, and 40 μg/mL anti-IFN-γ mAb, Treg: 5 ng/mL TGF-β, 2 nM trans-Retinoic Acid (Calbiochem, San Diego, CA, USA), 40 μg/mL anti-IL-4 mAb, and 40 μg/mL anti-IFN-γ mAb.…”

mentioning

confidence: 99%

“…Essentially the same responses were observed in both the #75 and #97 lines, though the stimulation-induced upregulation of EGFP fluorescence was hardly detectable by microscopic analysis.The difference in the reporter activity of established NFAT reporter mouse lines was further evaluated in differentiated helper T (Th)1, Th2, Th9, Th17, and regulatory T (Treg) cells. Th1 cells differentiated by our procedure preferentially express IFN-γ and CXCR3, Th2 express IL-4, IL-5, IL-13, and CCR4, Th9 express IL-9, Th17 express IL-17A, IL-22, and RORγt, and Treg express TGF-β1, IL-10, CTLA-4, CD25, and FoxP3 as described previously[7][8][9][10][11][12]. As shown in Fig.3, The expression of EGFP was upregulated by CD3/CD28 and PMA + IOM in all subsets.…”

mentioning

confidence: 99%

Generation of reporter mice for detecting the transcriptional activity of nuclear factor of activated T cells

et al. 2023

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Nuclear factor of activated T cells (NFAT) is a transcription factor essential for immunological and other biological responses. To develop analyzing system for NFAT activity in vitro and in vivo, we generated reporter mouse lines introduced with NFAT-driven enhanced green fluorescent protein (EGFP) expressing gene construct. Six tandem repeats of -286 to -265 of the human IL2 gene to which NFAT binds in association with its co-transcription factor, activator protein (AP)-1, was conjunct with thymidine kinase minimum promoter and following EGFP coding sequence. Upon introduction of the resulting reporter cassette into C57BL/6 fertilized eggs, the transgenic mice were obtained. Among 7 transgene-positive mice in 110 mice bone, 2 mice showed the designated reporter mouse character. Thus, the EGFP fluorescence of CD4 + and CD8 + T cells in these mice was enhanced by stimulation through CD3 and CD28. Each of phorbol 12-myristate 13-acetate (PMA) and ionomycin (IOM) stimulation weakly but their combined stimulation strongly enhanced EGFP expression. The stimulation-induced EGFP upregulation was also observed following T cell subset differentiation in a different manner. The EGFP induction by PMA + IOM stimulation was more potent than that by CD3/CD28 stimulation in helper T (Th)1, Th2, Th9, and regulatory T cells, while both stimulation conditions displayed the equivalent EGFP induction in Th17 cells. Our NFAT reporter mouse lines are useful for analyzing stimulation-induced transcriptional activation mediated by NFAT in cooperation with AP-1 in T cells.

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Changes in peripheral blood IL-9, Th9, and BAFF levels in patients with allergic rhinitis and their clinical implications

Liu,

Wang,

Mao

2024

THC

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BACKGROUND: Allergic Rhinitis (AR), a prevalent condition in otorhinolaryngology, is mediated by Type 1 hypersensitivity through IgE, characterized by Type 2 inflammatory response and eosinophil infiltration in the nasal mucosa. Since AR disease exhibits significant heterogeneity in symptom severity, an objective assessment of AR severity may facilitate better individualized treatment. OBJECTIVE: To explore the changes in peripheral blood IL-9, Th9, and BAFF levels of allergic rhinitis (AR) in patients and the clinical significance associated with it. METHODS: A retrospective study selected 80 AR patients admitted from January 2022 to October 2022 as the case group, dividing them into mild and moderate-to-severe groups based on symptom scores. Concurrently, 50 patients without AR, who were treated for nasal bone fractures or underwent septoplasty, were selected as the group for comparison. Alterations in the expression levels of peripheral blood IL-9, Th9, and BAFF were analyzed and compared among the different groups. The diagnostic value of serum BAFF for the severity of AR was analyzed using the receiver operating characteristic (ROC) curve. RESULTS: Noticeable variations were observed in clinical variables among the three groups such as, total IgE levels, peripheral blood eosinophil count and proportion, TNSS, and VAS (P< 0.05), while no statistically significant differences were observed in other variables (P> 0.05). The comparison of IL-9, Th9, and BAFF among the three groups revealed statistically significant differences (P< 0.05). Analysis using multivariate logistic regression revealed that IL-9 (OR = 2.365), Th9 (OR = 2.186), BAFF (OR = 2.307) were influencing factors of moderate-to-severe AR (P< 0.05). The ROC curve indicated that the AUC for the diagnosis of moderate-to-severe AR by IL-9, Th9, BAFF were 0.770, 0.734, 0.761, respectively, and the combined detection AUC was 0.888, an area under the curve higher than individual testing. CONCLUSION: Changes in peripheral blood IL-9, Th9, and BAFF levels in AR patients may function as indicators to assess the level of severity in diagnostic procedures.

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Suppressive effect of dexamethasone on murine Th9 cell-mediated nasal eosinophilic inflammation

Cited by 6 publications

References 11 publications

Microbiome profiling of nasal extracellular vesicles in patients with allergic rhinitis

Microbiome profiling of nasal extracellular vesicles in patients with allergic rhinitis

Generation of reporter mice for detecting the transcriptional activity of nuclear factor of activated T cells

Changes in peripheral blood IL-9, Th9, and BAFF levels in patients with allergic rhinitis and their clinical implications

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