Suppressive effect of compost bacteria against grey mould and Rhizopus rot on strawberry fruit

Cloutier, Ashley; Tran, Shirley; Avis, Tyler J.

doi:10.1080/09583157.2019.1695745

Cited by 9 publications

(4 citation statements)

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“…group G strain 87‐202‐3 [31,32]. Previous studies from our lab showed that A. humicola inhibited the growth of R. stolonifer , A. alternata , B. cinerea , and V. dahliae whereas A. psychrophenolicus inhibited B. cinerea and V. dahliae [8,9,18]. To the best of our knowledge, this is the first report of A. humicola inhibiting R. solani , and S. sclerotiorum and A. psychrophenolicus inhibiting the growth of R. solani , R. stolonifer , and S. sclerotiorum .…”

Section: Discussionmentioning

confidence: 77%

“…To mitigate the health risks, environmental concerns, and pathogen resistance associated with synthetic chemical pesticides, microbial antagonists have been previously tested to determine their antagonistic properties against plant pathogens [8,9,18]. In this study, the antifungal and antioomycetal activity of A. humicola strains M9‐1A, M9‐2, and M9‐8, and A. psychrophenolicus M9‐17 were tested against several important crop pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…A promising alternative to fungicides is biological control. Among biological control methods, antagonistic bacteria have previously demonstrated effective suppression of postharvest plant diseases caused by pathogenic fungi [8–10]. Biological control agents typically combat phytopathogens through induced plant resistance, competition for nutrients and space, parasitism, and/or antibiosis [11–13].…”

Section: Introductionmentioning

confidence: 99%

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Biological and chemical characterization of antimicrobial activity in Arthrobacter spp. isolated from disease‐suppressive compost

et al. 2021

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Antagonistic bacteria can act as biocontrol agents against various phytopathogens. Recently, Arthrobacter spp. demonstrated antifungal activity, but were not further characterized. In this study, the antimicrobial activity of Arthrobacter humicola strains M9‐1A, M9‐2, and M9‐8, and Arthrobacter psychrophenolicus strain M9‐17 were evaluated against nine plant pathogens in vitro, and their cell‐free filtrates were additionally assessed for inhibition of Alternaria alternata and suppression of black mold disease on tomato fruit. Results indicated that A. humicola M9‐1A and A. psychrophenolicus M9‐17 were the most inhibitory, reducing growth of seven of the pathogens studied. Cell‐free filtrates of A. psychrophenolicus M9‐17 reduced the growth of most pathogens. All cell‐free bacterial filtrates, except those from A. humicola M9‐2, suppressed black mold on tomato fruit. Disk diffusion assays with ethyl acetate soluble culture filtrate extracts of all bacteria reduced the mycelial growth of A. alternata. Clear inhibition zones were observed for A. psychrophenolicus M9‐17 extracts using drop bioassays. The antifungal compound N‐acetyltryptamine was purified and characterized from the A. psychrophenolicus M9‐17 cell‐free ethyl acetate soluble extract. This study suggests that antibiosis may play a key role in the antimicrobial activity of Arthrobacter spp.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological and chemical characterization of antimicrobial activity in Arthrobacter spp. isolated from disease‐suppressive compost

et al. 2021

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“…Rhizopus is fast-growing and their infection of fruits and other types of food is characterized by white mycelia and black sporangiospores during the decay process; another principal characteristic is the formation of rhizoids [ 39 , 40 ]. Some Rhizopus species, such as R. oryzae and R. stolonifera , are weak parasites of ripening honeybee-pollinated fruit crops, including apple, peach, strawberry, citrus, persimmon, pear, and pumpkin [ 39 , 41 , 42 , 43 , 44 ]. Following the bees’ foraging activities on infected crops, fungal spores gain entry and become established in the beehive by dissemination through direct contact and food contamination [ 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

A New Isolated Fungus and Its Pathogenicity for Apis mellifera Brood in China

Aynalem,

Meng,

Getachew

et al. 2024

Microorganisms

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In this article, we report the pathogenicity of a new strain of fungus, Rhizopus oryzae to honeybee larvae, isolated from the chalkbrood-diseased mummies of honeybee larvae and pupae collected from apiaries in China. Based on morphological observation and internal transcribed spacer (ITS) region analyses, the isolated pathogenic fungus was identified as R. oryzae. Koch’s postulates were performed to determine the cause-and-effect pathogenicity of this isolate fungus. The in vitro pathogenicity of this virulent fungus in honeybees was tested by artificially inoculating worker larvae in the lab. The pathogenicity of this new fungus for honeybee larvae was both conidial-concentration and exposure-time dependent; its highly infectious and virulent effect against the larvae was observed at 1 × 105 conidia/larva in vitro after 96 h of challenge. Using probit regression analysis, the LT50 value against the larvae was 26.8 h at a conidial concentration of 1 × 105 conidia/larva, and the LC50 was 6.2 × 103 conidia/larva. These results indicate that the new isolate of R. oryzae has considerable pathogenicity in honeybee larvae. Additionally, this report suggests that pathogenic phytofungi may harm their associated pollinators. We recommend further research to quantify the levels, mechanisms, and pathways of the pathogenicity of this novel isolated pathogen for honeybee larvae at the colony level.

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