Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation

Wang, Hui‐Fang; Liu, Jinling; Jiang, Xinli; Lü, Jin-Chun; Li, Xiaolei; Song, Xiujun

doi:10.1007/s10384-011-0060-0

Cited by 1 publication

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“…Tolerance-inducing DCs are considered to be less differentiated than immunogenic DCs (Siepmann et al, 2007). DCs in the eye are maintained in an immature state by transforming growth factor beta-2 (TGF-b2), which is constitutively present in the aqueous humor (Streilein et al, 2002;Wang et al, 2011). Antigen presentation by DCs can induce Treg generation (Cobbold et al, 2003;Jiang et al, 2004;Yamazaki et al, 2006;Golshayan et al, 2007;Yamazaki and Morita, 2013).…”

Section: Introductionmentioning

confidence: 99%

Tolerogenic dendritic cells suppress murine corneal allograft rejection by modulating CD28/CTLA‐4 expression on regulatory T cells

Yan

Cai

Hui

et al. 2014

Cell Biology International

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Anterior chamber-associated immune deviation (ACAID) is initiated when dendritic cells (DCs) capture intraocular antigen and induce regulatory T cells (Tregs) in the spleen. We investigated whether DCs could be used as an immunotherapy to prevent murine corneal allograft rejection by inducing Tregs. Bone marrow-derived DCs (BMDCs) were differentiated with transforming growth factor-β2 (TGF-β2) in the presence of donor-derived allopeptide in vitro (TGFβ2-DCs), and adoptively transferred to BALB/c mice. Three days later a corneal allograft transplant was carried out. Graft rejection, as well as the number and the phenotype of splenic Tregs, were determined after transplant. CD86, CD80, CD40 were present, and MHC class II expression were significantly reduced on TGFβ2-DCs compared to BMDCs not exposed to TGF-β2. TGFβ2-DCs increased the number of splenic Tregs (CD4(+)CD25(+)) in recipient mice prior to transplant by modulating CD28/CTLA-4 expression. These induced Tregs suppressed proliferation of CD4(+)CD25(-) T cells ex vivo. TGFβ2-DCs delayed corneal allograft rejection and TGFβ2-DC recipient mice had significantly more splenic Tregs expressing Foxp3 and CTLA-4, but fewer CD28+ Tregs. Expression of GITR, CD69, and CD45RB were similar in TGFβ2-DC and control mice. Therefore, the phenotype of TGFβ2-DCs suggests they are tolerogenic DCs (tolDCs). In vivo, these cells increased the number and function of Tregs by modulating CD28/CTLA-4 expression. The suppressor Tregs induced by TGFβ2-DCs may be involved in the induction of ACAID, which helps to suppress corneal allograft rejection. Our results provide proof of principle for the use of tolDCs as immunotherapy during transplant.

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Section: Introductionmentioning

confidence: 99%

Tolerogenic dendritic cells suppress murine corneal allograft rejection by modulating CD28/CTLA‐4 expression on regulatory T cells

Yan

Cai

Hui

et al. 2014

Cell Biology International

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Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation

Abstract: These results reveal that TGF-β(2) in the AH is of crucial importance in maintaining DC in the immature state. Further experiments will clarify the immune role of PGE(2) in AH.

Cited by 1 publication

References 26 publications

Tolerogenic dendritic cells suppress murine corneal allograft rejection by modulating CD28/CTLA‐4 expression on regulatory T cells

Tolerogenic dendritic cells suppress murine corneal allograft rejection by modulating CD28/CTLA‐4 expression on regulatory T cells

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