Suppressive activity of bone marrow cells from patients with stomach cancer. Effect of prostaglandins, transforming growth factor-β, and nitric oxide

Abstract

It is shown that nonadhesive bone marrow ceils from patients with stomach cancer suppress phytohemagglutinin-stimulated proliferation of peripheral blood lymphocytes of healthy donors and proliferation of Molt-4 human lymphoma cells in vitro. Suppressive activity of bone marrow cells from cancer patients is not mediated through prostaglandin secretion, since indomethacin has no effect on it. Addition of neutralizing monoclonal antibodies to transforming growth factor-13 t, 132, and 133 partially reduces this suppressive effect. Suppressive effect of bone marrow cells from patients with stomach cancer is partially mediated through production of nitric oxide, since the inhibitor of its synthesis Ng-monomethyl-Larginine diminishes the inhibiting effect of bone marrow cells from cancer patients on phytohemagglutinin-stimulated proliferation of peripheral blood T cells from healthy donors.