Suppressive activities and mechanisms of ugonin J on vascular smooth muscle cells and balloon angioplasty‐induced neointimal hyperplasia

Abstract

Neointimal hyperplasia (or restenosis) is primarily attributed to excessive proliferation and migration of vascular smooth muscle cells (VSMCs). In this study, we investigated the inhibitory effects and mechanisms of ugonin J on VSMC proliferation and migration as well as neointimal formation. Cell viability and the cell-cycle distribution were, respectively, analyzed using an MTT assay and flow cytometry. Cell migration was examined using a wound-healing analysis and a transwell assay. Protein expressions and gelatinase activities were, respectively, measured using Western blot and gelatin zymography. Balloon angioplasty-induced neointimal formation was induced in a rat carotid artery model and then examined using immunohistochemical staining. Ugonin J induced cell-cycle arrest at the G /G phase and apoptosis to inhibit VSMC growth. Ugonin J also exhibited marked suppressive activity on VSMC migration. Ugonin J significantly reduced activations of focal adhesion kinase, phosphoinositide 3-kinase, v-akt murine thymoma viral oncogene homolog 1, and extracellular signal-regulated kinase 1/2 proteins. Moreover, ugonin J obviously reduced expressions and activity levels of matrix metalloproteinase-2 and matrix metalloproteinase-9. In vivo data indicated that ugonin J prevented balloon angioplasty-induced neointimal hyperplasia. Our study suggested that ugonin J has the potential for application in the prevention of balloon injury-induced neointimal formation.

RING finger protein�10 is a potential drug target for diabetic vascular complications

“…20536; ProteinTech). Enhanced chemiluminescence development methods (Advansta Inc., Menlo Park, CA, USA) were used to visualize the immunoblotting bands (19). The bands were analyzed with ImageLab 3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA).…”

Helminthostachys zeylanica alleviates hepatic steatosis and insulin resistance in diet-induced obese mice

“…Furthermore, HZ extract was considered to have neuroprotective activity because of its anti-inflammatory activity on human astrocytes, through bradykinin-induced MMP-9 signaling [13]. Another bioactive compound extracted from HZ, ugonin J, is considered to be a potential inhibitor of cell migration and neointima formation through MMP-2 and -9 pathways [14]. Rhizomes of HZ have been used for variety of purposes, including protection against liver damage [15].…”

Antiulcer effect of Helminthostachys zeylanica (L.) Hook. root extract on ethanol-induced acute gastric lesion in Wistar rats

“…(family Ophioglossaceae), known as Tunjuk Langit in Indonesia, is biologically rich in potential flavonoids such as ugonin A-Z (Chen et al, 2003;Huang et al, 2003; and ugonstilben, which can be used as an alternative medicine for peptic ulcers because of their antioxidants and anti-inflammatory activities. Previous studies on the pharmacological effects of H. zeylanica extracts include hepatoprotective (Suja et al, 2004;Wu et al, 2018), cytotoxic and antioxidant , anti-hyperuricemia (Fitrya and Muharni, 2014), anti-inflammatory (Hsieh et al, 2016;Wu et al, 2017b), anti-melanogenesis (Yamauchi et al, 2015), antiosteoporosis (Huang et al, 2017), anti-acute lung inflammation (Liou et al, 2017), suppressive vascular abnormalities (Pan et al, 2018), antisteatosis (Chang et al, 2019), and antidiabetic (Ridhasya et al, 2019). However, there are no studies on the potential of its extract as an antiulcer.…”